Abstract
Acute lung injury is characterized by lung edema, surfactant dysfunction, and inflammation. The main goal of our study was to evaluate effects of S-nitroso-N-acetyl-penicillamine (SNAP) on migration of cells into the lung and their activation, inducible NO synthase (iNOS) activity, and apoptosis in experimental acute lung injury (ALI) in rabbits. ALI was induced by repetitive lung lavage with saline. The animals were divided into the following groups: (1) ALI without therapy, (2) lung injury treated with SNAP (ALI + SNAP), and (3) healthy animals (Control). After 5 h of ventilation, total and differential counts of cells in the bronchoalveolar lavage fluid (BALF) were assessed. Concentrations of interleukins (IL)-1ß, IL-6, and IL-8, endogenous secretory receptor for advanced glycation endproducts (esRAGE), sphingosine-1-phosphate receptor (S1PR)3, caspase-3, and mRNA expression of inducible NO synthase (iNOS) in lung tissue and nitrite/nitrate in plasma were analyzed. In the right lung, apoptotic cells were evaluated by TUNEL assay. In the animals with ALI, higher counts of cells, mainly neutrophils, in BALF and increased production of pro-inflammatory substances were observed compared with controls. SNAP therapy reduced a leak of cells into the lung and decreased concentrations of pro-inflammatory and apoptotic markers, reduced mRNA expression of iNOS, and decreased apoptotic index in the lung.
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Acknowledgements
The authors thank D. Kuliskova, Z. Remisova, M. Petraskova, and M. Hutko for technical assistance. In addition, we would like to thank for support by grants APVV-0435-11, APVV-15-0075, VEGA 1/0305/14, BioMed (ITMS 26220220187), and UK/28/2015.
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The authors declare no conflict of interest in relation to this article.
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Kosutova, P., Mikolka, P., Kolomaznik, M., Balentova, S., Calkovska, A., Mokra, D. (2016). Effects of S-Nitroso-N-Acetyl-Penicillamine (SNAP) on Inflammation, Lung Tissue Apoptosis and iNOS Activity in a Rabbit Model of Acute Lung Injury. In: Pokorski, M. (eds) Pulmonary Infection and Inflammation. Advances in Experimental Medicine and Biology(), vol 935. Springer, Cham. https://doi.org/10.1007/5584_2016_34
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DOI: https://doi.org/10.1007/5584_2016_34
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