Virological Characteristics of the 2014/2015 Influenza Season Based on Molecular Analysis of Biological Material Derived from I-MOVE Study
The main goal of the international study I-MOVE (Influenza Monitoring of Vaccine Effectiveness) implemented in Poland is to identify and evaluate the activity types of influenza virus and to determine the effectiveness of vaccination against influenza in the 2014–2015 influenza season. The study is based on selecting patients with flu symptoms and collecting biological samples for laboratory examination. Detection, typing, and subtyping of influenza viruses were carried out by the National Center for Influenza Virus Research at National Institute of Public Health – National Institute of Hygiene, serving as a reference center, and also in selected laboratories of the Regional Sanitary Epidemiological Stations. Molecular biology methods, such as reverse transcription polymerase chain reaction (RT-PCR), were applied in this study. A total of 218 samples were collected. A hundred and twenty six samples, representing 57.8 % of the total, were confirmed with influenza virus infection. Influenza type A virus was detected in 54 samples, which included 16 samples of A/H1N1/pdm09 subtype and 11 samples of A/H3N2/ subtype. The remaining 27 samples positive for influenza type A were not subtyped. Influenza type B virus was detected in 57 samples, which appeared to be the dominant strain in this study. Furthermore, several cases of concurrent infection with influenza type B virus and the A/H1N/pdm09 or A/H3N2/ subtype were observed.
KeywordsInfluenza season Influenza virus Molecular biology Vaccination Virology Virus subtype
This work was funded in parts by Specific Contract No 1-ECD.5235, implementing activities to the Framework Contract No/ECDC/2014/026, and NIPH-NIH thematic grant 5/EM.1
Conficts of Interest
The authors declare no conflicts of interest in relation to this article.
- Kissling E, Valenciano M, I-MOVE case–control studies team (2012) Early estimates of seasonal influenza vaccine effectiveness in Europe among target groups for vaccination: results from the I-MOVE multicentre case-control study, 2011/12. Euro Surveill 17(15):pii=20146Google Scholar
- Valenciano M, Kissling E, Reuss A, Jiménez-Jorge S, Horváth JK, Donnell JMO, Pitigoi D, Machado A, Pozo F, I-MOVE Multicentre Case Control Study Team (2015) The European I-MOVE Multicentre 2013–2014 Case-control Study. Homogeneous moderate influenza vaccine effectiveness against A(H1N1)pdm09 and heterogeneous results by country against A(H3N2). Vaccine 33(24):2813–2822CrossRefPubMedGoogle Scholar