Thermal Sensitivity and Dimethyl Sulfoxide (DMSO)

  • Kotaro Takeda
  • Mieczyslaw PokorskiEmail author
  • Yasumasa Okada
Part of the Advances in Experimental Medicine and Biology book series (AEMB, volume 921)


Dimethyl sulfoxide (DMSO) is commonly used as a solvent for hydrophobic substances, but the compound’s innate bioactivity is an area of limited understanding. In this investigation we seek to determine the analgesic potential of DMSO. We addressed the issue by assessing the perception of thermal pain stimulus, using a 55 °C hotplate design, in conscious mice. The latency of withdrawal behaviors over a range of incremental accumulative intraperitoneal DMSO doses (0.5–15.5 g/kg) in the same mouse was taken as a measure of thermal endurance. The findings were that the latency, on average, amounted to 15–30 s and it differed inappreciably between the sequential DMSO conditions. Nor was it different from the pre-DMSO control conditions. Thus, DMSO did not influence the cutaneous thermal pain perception. The findings do not lend support to those literature reports that point to the plausible antinociceptive potential of DMSO as one of a plethora of its innate bioactivities. However, the findings concern the mouse’s footpad nociceptors which have specific morphology and stimulus transduction pathways, which cannot exclude DMSO’s antinociceptive influence on other types of pain or in other types of skin. Complex and as yet unresolved neural mechanisms of perception of cutaneous noxious heat stimulus should be further explored with alternative experimental designs.


Analgesia Dimethyl sulfoxide Nociception Noxious heat Pain Perception Thermal stimulus 



This work was supported by JSPS KAKENHI Grants 25540130, 26460311, 26670676, 15K00417, 15K12611, Health and Labor Sciences Research Grants of Japan, and JSPS Japan-Poland Researcher Exchange Program Grant.

Conflict of Interests

None declared.


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Copyright information

© Springer International Publishing Switzerland 2016

Authors and Affiliations

  • Kotaro Takeda
    • 1
    • 2
  • Mieczyslaw Pokorski
    • 1
    • 3
    Email author
  • Yasumasa Okada
    • 1
  1. 1.Clinical Research CenterNational Hospital Organization Murayama Medical CenterTokyoJapan
  2. 2.Fujita Memorial Nanakuri InstituteFujita Health UniversityTsuJapan
  3. 3.Public Higher Medical Professional School in OpoleOpolePoland

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