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Inhibition of Urease Enzyme Production and some Other Virulence Factors Expression in Proteus mirabilis by N-Acetyl Cysteine and Dipropyl Disulphide

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Advances in Microbiology, Infectious Diseases and Public Health

Part of the book series: Advances in Experimental Medicine and Biology ((AMIDPH,volume 973))

Abstract

Proteus mirabilis is one of the important pathogens that colonize the urinary tract and catheters resulting in various complications, such as blockage of the catheters and the formation of infective stones. Purpose: In this study we evaluated the effect of N-acetyl cysteine (NAC) and dipropyl disulphide on some virulence factors expressed by a Proteus mirabilis strain isolated from a catheterized patient. Methods: Antibacterial activity of both compounds was determined by broth microdilution method. Their effect on different types of motility was determined by LB medium with variable agar content and sub-MIC of each drug. Their effect on adherence and mature biofilms was tested by tissue culture plate assay. Inhibitory effect on urease production was determined and supported by molecular docking studies. Results: The minimum inhibitory concentration (MIC) of NAC and dipropyl disulphide was 25 mM and 100 mM, respectively. Both compounds decreased the swarming ability and biofilm formation of the tested isolate in a dose-dependent manner. NAC had higher urease inhibitory activity (IC50 249 ±0.05 mM) than that shown by dipropyl disulphide (IC50 10±0.2 mM). Results were supported by molecular docking studies which showed that NAC and dipropyl disulphide interacted with urease enzyme with binding free energy of −4.8 and −8.528 kcal/mol, respectively. Docking studies showed that both compounds interacted with Ni ion and several amino acids (His-138, Gly-279, Cysteine-321, Met-366 and His-322) which are essential for the enzyme activity. Conclusion: NAC and dipropyl disulphide could be used in the control of P. mirabilis urinary tract infections.

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Funding

This work was supported by the authors themselves.

Conflict of Interest

The authors declare that they have no conflict of interest.

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Authors and Affiliations

  1. Department of Microbiology and Immunology, Faculty of Pharmacy, Minia University, 61519, Minia, Egypt

    Rehab Mahmoud Abdel-Baky

  2. Department of Medicinal Chemistry, Faculty of Pharmacy, Minia University, 61519, Minia, Egypt

    Mohamed Abdullah Ali & Gamal El-Din Ali A. Abuo-Rahma

  3. Department of Microbiology and Immunology, Faculty of Pharmacy, Ahram Canadian University, 12581, Sixth of October City, Egypt

    Neveen AbdelAziz

Authors
  1. Rehab Mahmoud Abdel-Baky
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  2. Mohamed Abdullah Ali
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  3. Gamal El-Din Ali A. Abuo-Rahma
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  4. Neveen AbdelAziz
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Correspondence to Neveen AbdelAziz .

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  1. Microbial Biofilm Laboratory, Fondazione Santa Lucia IRCCS, Rome, Italy

    Gianfranco Donelli

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Abdel-Baky, R.M., Ali, M.A., Abuo-Rahma, G.ED.A.A., AbdelAziz, N. (2017). Inhibition of Urease Enzyme Production and some Other Virulence Factors Expression in Proteus mirabilis by N-Acetyl Cysteine and Dipropyl Disulphide. In: Donelli, G. (eds) Advances in Microbiology, Infectious Diseases and Public Health. Advances in Experimental Medicine and Biology(), vol 973. Springer, Cham. https://doi.org/10.1007/5584_2016_197

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