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STED and RESOLFT Fluorescent Nanoscopy

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Fluorescence Spectroscopy and Microscopy in Biology

Part of the book series: Springer Series on Fluorescence ((SS FLUOR,volume 20))

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Abstract

Fluorescence microscopy is an invaluable tool in cell biology to investigate the functional, structural, and dynamical properties of biological specimens. For a long time, the resolution of fluorescence microscopes was thought to be fundamentally limited by diffraction. According to Abbe’s law of diffraction, published in 1873, the smallest spatial details accessible with visible light are defined only by the optics of the microscope, i.e., numerical aperture and the wavelength of the light. However, the last 25 years of research have shown that it is possible to investigate even smaller structures using only visible light. This chapter covers the basic principles of coordinate-targeted switching techniques, a family of super-resolution microscopy methods. Furthermore, it provides an overview of the state-of-the-art strategies to push their ability toward faster and more efficient imaging of living cells and tissue.

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Authors and Affiliations

  1. Science for Life Laboratory, KTH Royal Institute of Technology, Stockholm, Sweden

    Andreas Bodén, Francesca Pennacchietti & Ilaria Testa

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  1. Andreas Bodén
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  2. Francesca Pennacchietti
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Correspondence to Ilaria Testa .

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  1. J. Heyrovský Institute of Physical Chemistry, Prague, Czech Republic

    Radek Šachl

  2. J. Heyrovský Institute of Physical Chemistry, Prague, Czech Republic

    Mariana Amaro

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Bodén, A., Pennacchietti, F., Testa, I. (2022). STED and RESOLFT Fluorescent Nanoscopy. In: Šachl, R., Amaro, M. (eds) Fluorescence Spectroscopy and Microscopy in Biology. Springer Series on Fluorescence, vol 20. Springer, Cham. https://doi.org/10.1007/4243_2022_35

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