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Instrumentation for Fluorescence Lifetime Measurement Using Photon Counting

Part of the Springer Series on Fluorescence book series (SS FLUOR,volume 18)

Abstract

We describe the evolution of HORIBA Jobin Yvon IBH Ltd, and its time-correlated single-photon counting (TCSPC) products, from university research beginnings through to its present place as a market leader in fluorescence lifetime spectroscopy. The company philosophy is to ensure leading-edge research capabilities continue to be incorporated into instruments in order to meet the needs of the diverse range of customer applications, which span a multitude of scientific and engineering disciplines. We illustrate some of the range of activities of a scientific instrument company in meeting this goal and highlight by way of an exemplar the performance of the versatile DeltaFlex instrument in measuring fluorescence lifetimes. This includes resolving fluorescence lifetimes down to 5 ps, as frequently observed in energy transfer, nanoparticle metrology with sub-nanometre resolution and measuring a fluorescence lifetime in as little as 60 μs for the study of transient species and kinetics.

Keywords

  • DeltaFlex
  • Fluorescence lifetime
  • HORIBA Jobin Yvon IBH
  • Photon counting
  • TCSPC

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Acknowledgement

PY wishes to thank the QuantIC Technology Hub for a research fellowship.

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Correspondence to David J. S. Birch .

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Birch, D.J.S., Hungerford, G., McLoskey, D., Sagoo, K., Yip, P. (2019). Instrumentation for Fluorescence Lifetime Measurement Using Photon Counting. In: Pedras, B. (eds) Fluorescence in Industry. Springer Series on Fluorescence, vol 18. Springer, Cham. https://doi.org/10.1007/4243_2018_2

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