Abstract
GPCR oligomerization has been a matter of intense research these last years. FRET and BRET methods have paved the way to a generalized concept of potential GPCR oligomerization in artificial systems (transfected cell lines). More recently, the use of fluorescent ligands compatible with time-resolved FRET studies has opened the possibility of GPCR oligomerization study in their native context and brought evidence of their existence. Furthermore, recent applications of original fluorescence techniques are unveiling new information on the dynamics that govern these complexes and are changing the way we see GPCR oligomeric structures.
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Abbreviations
- ACP:
-
Acyl carrier protein
- BiFC:
-
Bimolecular fluorescence complementation
- BRET:
-
Bioluminescence resonance energy transfer
- CFP:
-
Cyan fluorescent protein
- co-IP:
-
Co-immunoprecipitation
- FCS:
-
Fluorescence correlation spectroscopy
- FP:
-
Fluorescent protein
- FRET:
-
Fluorescence resonance energy transfer
- GABA:
-
γ-Aminobutyric acid
- GFP:
-
Green fluorescent protein
- GPCR:
-
G-protein-coupled receptor
- hAGT:
-
O6-Alkylguanine-DNA alkyltransferase
- HTS:
-
High-throughput screening
- pbFRET:
-
Photobleaching FRET
- RET:
-
Resonance energy transfer
- Rluc:
-
Renilla luciferase
- RTK:
-
Receptor tyrosine kinase
- smFRET:
-
Single-molecule FRET
- SPT:
-
Single-particle tracking
- TIRF:
-
Total internal reflection fluorescence
- TR-FRET:
-
Time-resolved FRET
- YFP:
-
Yellow fluorescent protein
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Cottet, M., Faklaris, O., Trinquet, E., Pin, JP., Durroux, T. (2012). New Fluorescent Strategies Shine Light on the Evolving Concept of GPCR Oligomerization. In: Mély, Y., Duportail, G. (eds) Fluorescent Methods to Study Biological Membranes. Springer Series on Fluorescence, vol 13. Springer, Berlin, Heidelberg. https://doi.org/10.1007/4243_2012_54
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