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Fluorescence Correlation Spectroscopy to Study Membrane Organization and Interactions

  • Monika Zelman-Femiak
  • Yamunadevi Subburaj
  • Ana J. García-SáezEmail author
Chapter
Part of the Springer Series on Fluorescence book series (SS FLUOR, volume 13)

Abstract

This chapter describes the application of fluorescence correlation spectroscopy (FCS) as a powerful technique for the study of membrane organization and interactions. Monitoring the fluorescence signal fluctuations allows resolving concentrations, diffusion coefficients, and binding of several membrane components in experiments in vitro as well as in vivo.

We discuss the basic principles of FCS and explain novel implementations of FCS introduced to overcome the technical difficulties present in the standard version of fluorescence correlation spectroscopy. Finally, we report several examples of studies with the application of FCS on both model and biological membranes to obtain interesting insight in the topic of lateral membrane organization and membrane interactions.

Keywords

Chemical relaxation Diffusion Fluctuations Fluorescence Fluorescence correlation spectroscopy Interactions Membranes Triplet state 

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Copyright information

© Springer-Verlag Berlin Heidelberg 2012

Authors and Affiliations

  • Monika Zelman-Femiak
    • 1
  • Yamunadevi Subburaj
    • 1
  • Ana J. García-Sáez
    • 1
    Email author
  1. 1.Max Planck Institute for Intelligent Systems and German Cancer Research Center, BioquantHeidelbergGermany

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