Flow Cytometry: Instrumentation, Applications, Future Trends and Limitations

Chapter

Abstract

Flow cytometry is the measurement of cells or other particles flowing past one or more sensing zones. Most commonly the sensing zone is a focused beam of light, and particles are characterized by the scattered and fluorescent light pulses that are created as the particle passes through the light beam. Unlike microscopic image analysis, flow cytometry generally measures the total signal from a particle and does not provide morphological detail on the shape or size of the sources of signal within the particle. The strength of flow cytometry is rapidly providing measurements from multiple fluorochrome stains along with several intrinsic measures of particle characteristics. In some flow cytometers particles with selected measurement properties can be physically sorted and collected. This chapter provides an overview of the technology and application of flow cytometry. All major aspects of the technology are introduced, and references to original literature are provided for readers interested in additional details. Special attention is given the use of fluorescence measurements.

Cell sorter Flow cytometry Fluorescence Immunofluorescence Laser Light scatter  

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References

  1. 1.
    Givan AL (2001) Flow Cytometry: First Principles. Wiley, New York Google Scholar
  2. 2.
    Ormerod MG (2000) Flow Cytometry: A Practical Approach. Oxford University Press, Oxford Google Scholar
  3. 3.
    Diamond RA, Demaggio S (eds.) (2000) In Living Color: Protocols in Flow Cytometry and Cell Sorting. Springer, Berlin Google Scholar
  4. 4.
    Watson JV (2004) Introduction to Flow Cytometry. Cambridge University Press, Cambridge Google Scholar
  5. 5.
    Shapiro HM (2003) Practical Flow Cytometry, 4th edn. Wiley, Hoboken Google Scholar
  6. 6.
    Kachel V (1976) J Histochem Cytochem 24:211 Google Scholar
  7. 7.
    Crosland-Taylor PJ (1953) Nature 171:37 Google Scholar
  8. 8.
    Crosland-Taylor PJ, Stewart JW, Haggis G (1958) Blood 13:398 Google Scholar
  9. 9.
    Dittrich W, Gohde W (1969) Z Naturforsch B 24:360 Google Scholar
  10. 10.
    Kamentsky LA, Melamed MR, Derman H (1965) Science 150:630 Google Scholar
  11. 11.
    Kamentsky LA, Melamed MR (1967) Science 156:1364 Google Scholar
  12. 12.
    Fulwyler MJ (1965) Science 150:910 Google Scholar
  13. 13.
    Sweet RG (1965) Rev Sci Instrum 36:131 Google Scholar
  14. 14.
    Hulett HR, Bonner WA, Barrett J et al. (1969) Science 166:747 Google Scholar
  15. 15.
    Stewart CC, Steinkamp JA (1982) Cytometry 2:238 CrossRefGoogle Scholar
  16. 16.
    Keij JF, van Rotterdam A, Groenewegen AC et al. (1991) Cytometry 12:398 Google Scholar
  17. 17.
    Leary JF (2005) Cytometry A 67:76 Google Scholar
  18. 18.
    Bonner WA, Hulett HR, Sweet RG et al. (1972) Rev Sci Instrum 43:404 Google Scholar
  19. 19.
    Hulett HR, Bonner WA, Sweet RG et al. (1973) Clin Chem 19:813 Google Scholar
  20. 20.
    Fu AY, Spence C, Scherer A et al. (1999) Nat Biotechnol 17:1109 Google Scholar
  21. 21.
    Habbersett RC, Jett JH (2004) Cytometry A 60:125 Google Scholar
  22. 22.
    Steen HB (2004) Cytometry A 57:94 Google Scholar
  23. 23.
    Kuckuck FW, Edwards BS, Sklar LA (2001) Cytometry 44:83 Google Scholar
  24. 24.
    Schwartz A, Sugg H, Ritter TW et al. (1983) Cytometry 3:456 Google Scholar
  25. 25.
    Steinkamp JA, Hiebert RD (1982) Cytometry 2:232 Google Scholar
  26. 26.
    Steinkamp JA, Kraemer PM (1974) J Cell Physiol 84:197 Google Scholar
  27. 27.
    Hoffman RA, Britt WB (1979) J Histochem Cytochem 27:234 Google Scholar
  28. 28.
    Hoffman RA, Johnson TS, Britt WB (1981) Cytometry 1:377 Google Scholar
  29. 29.
    Warner BA, Reardon DM (1991) Am J Clin Pathol 95:207 Google Scholar
  30. 30.
    Peters DC (1979) J Histochem Cytochem 27:241 Google Scholar
  31. 31.
    Goodwin PM, Ambrose WP, Martin JC et al. (1995) Cytometry 21:133 Google Scholar
  32. 32.
    Asbury CL, Uy JL, van den Engh G (2000) Cytometry 40:88 Google Scholar
  33. 33.
    Beisker W, Eisert WG (1981) Anal Quant Cytol 3:315 Google Scholar
  34. 34.
    Beisker W (1997) Proc SPIE 2982:420 Google Scholar
  35. 35.
    Hakamata T (2006) Photomultiplier Tubes – Basics and Applications. Hamamatsu Photonics K.K. Electron Tube Division, Hamamatsu City Google Scholar
  36. 36.
    Kerker M (1983) Cytometry 4:1 Google Scholar
  37. 37.
    Salzman GC, Wilder ME, Jett JH (1979) J Histochem Cytochem 27:264 Google Scholar
  38. 38.
    Mullaney PF, Crowell JM, Salzman GC et al. (1976) J Histochem Cytochem 24:298 Google Scholar
  39. 39.
    Sharpless TK, Bartholdi M, Melamed MR (1977) J Histochem Cytochem 25:845 Google Scholar
  40. 40.
    Steinkamp JA (1983) Cytometry 4:83 Google Scholar
  41. 41.
    Stewart CC, Stewart SJ, Habbersett RC (1989) Cytometry 10:426 Google Scholar
  42. 42.
    de Grooth BG, Terstappen LW, Puppels GJ et al. (1987) Cytometry 8:539 Google Scholar
  43. 43.
    Fawzi ZO, Fakhro NA, Nabhan RA et al. (2003) Trans R Soc Trop Med Hyg 97:71 Google Scholar
  44. 44.
    Olson RJ, Zettler ER, Anderson OK (1989) Cytometry 10:636 Google Scholar
  45. 45.
    Leary JF, Todd P, Wood JC et al. (1979) J Histochem Cytochem 27:315 Google Scholar
  46. 46.
    Sharpless TK, Melamed MR (1976) J Histochem Cytochem 24:257 Google Scholar
  47. 47.
    Maecker HT, Frey T, Nomura LE et al. (2004) Cytometry A 62:169 Google Scholar
  48. 48.
    Bagwell CB, Adams EG (1993) Ann NY Acad Sci 677:167 Google Scholar
  49. 49.
    Roederer M (2001) Cytometry 46:357 Google Scholar
  50. 50.
    Roederer M (2001) Cytometry 45:194 Google Scholar
  51. 51.
    Steinkamp JA, Crissman HA (1993) Cytometry 14:210 Google Scholar
  52. 52.
    Cui HH, Valdez JG, Steinkamp JA et al. (2003) Cytometry A 52:46 Google Scholar
  53. 53.
    Deka C, Cram LS, Habbersett R et al. (1995) Cytometry 21:318 Google Scholar
  54. 54.
    Deka C, Sklar LA, Steinkamp JA (1994) Cytometry 17:94 Google Scholar
  55. 55.
    Pinsky BG, Ladasky JJ, Lakowicz JR et al. (1993) Cytometry 14:123 Google Scholar
  56. 56.
    Steinkamp JA (1994) Methods Cell Biol 42 Pt B:627 Google Scholar
  57. 57.
    Crissman HA, Steinkamp JA (2001) Methods Cell Biol 63:131 CrossRefGoogle Scholar
  58. 58.
    Arndt-Jovin DJ, Jovin TM (1976) Prog Clin Biol Res 9:123 Google Scholar
  59. 59.
    Keene JP, Hodgson BW (1980) Cytometry 1:118 Google Scholar
  60. 60.
    Schaap GH, de Josselin de Jong JE, Jongkind JF (1984) Cytometry 5:188 Google Scholar
  61. 61.
    Bock G, Huber LA, Wick G et al. (1989) J Histochem Cytochem 37:1653 Google Scholar
  62. 62.
    Epstein M, Norman A, Pinkel D et al. (1977) J Histochem Cytochem 25:821 Google Scholar
  63. 63.
    Fox MH, Delohery TM (1987) Cytometry 8:20 Google Scholar
  64. 64.
    Bene L, Fulwyler MJ, Damjanovich S (2000) Cytometry 40:292 Google Scholar
  65. 65.
    Chan SS, Arndt-Jovin DJ, Jovin TM (1979) J Histochem Cytochem 27:56 Google Scholar
  66. 66.
    Uy JL, Asbury CL, Petersen TW et al. (2004) Cytometry A 61:18 Google Scholar
  67. 67.
    Seamer LC, Bagwell CB, Barden L et al. (1997) Cytometry 28:118 Google Scholar
  68. 68.
    Wood JC (1998) Cytometry 33:260 Google Scholar
  69. 69.
    Bagwell CB (2005) Cytometry A 64:34 Google Scholar
  70. 70.
    Parks DR, Roederer M, Moore WA (2006) Cytometry A 69:541 Google Scholar
  71. 71.
    Aubin JE (1979) J Histochem Cytochem 27:36 Google Scholar
  72. 72.
    Benson RC, Meyer RA, Zaruba ME et al. (1979) J Histochem Cytochem 27:44 Google Scholar
  73. 73.
    White JC, Stryer L (1987) Anal Biochem 161:442 Google Scholar
  74. 74.
    Doornbos RM, de Grooth BG, Greve J (1997) Cytometry 29:204 Google Scholar
  75. 75.
    Bagwell CB, Baker D, Whetstone S et al. (1989) Cytometry 10:689 Google Scholar
  76. 76.
    Gandler W, Shapiro H (1990) Cytometry 11:447 Google Scholar
  77. 77.
    Schmid I, Schmid P, Giorgi JV (1988) Cytometry 9:533 Google Scholar
  78. 78.
    Wood JC, Hoffman RA (1998) Cytometry 33:256 Google Scholar
  79. 79.
    Chase ES, Hoffman RA (1998) Cytometry 33:267 Google Scholar
  80. 80.
    Steen HB (1992) Cytometry 13:822 Google Scholar
  81. 81.
    Jung T, Schauer U, Heusser C et al. (1993) J Immunol Methods 159:197 Google Scholar
  82. 82.
    Prussin C, Metcalfe DD (1995) J Immunol Methods 188:117 Google Scholar
  83. 83.
    Nylander S, Kalies I (1999) J Immunol Methods 224:69 Google Scholar
  84. 84.
    Maino VC, Maecker HT (2004) Clin Immunol 110:222 Google Scholar
  85. 85.
    Suni MA, Maino VC, Maecker HT (2005) Curr Opin Immunol 17:434 Google Scholar
  86. 86.
    Maecker HT, Rinfret A, D'Souza P et al. (2005) BMC Immunol 6:13 Google Scholar
  87. 87.
    Gratama JW, Orfao A, Barnett D et al. (1998) Cytometry 34:128 Google Scholar
  88. 88.
    Baumgarth N, Roederer M (2000) J Immunol Methods 243:77 Google Scholar
  89. 89.
    Roederer M, De Rosa S, Gerstein R et al. (1997) Cytometry 29:328 Google Scholar
  90. 90.
    Roederer M, Brenchley JM, Betts MR et al. (2004) Clin Immunol 110:199 Google Scholar
  91. 91.
    Wood B (2006) Arch Pathol Lab Med 130:680 Google Scholar
  92. 92.
    Lisi PJ, Huang CW, Hoffman RA et al. (1982) Clin Chim Acta 120:171 Google Scholar
  93. 93.
    McHugh TM, Stites DP, Casavant CH et al. (1986) J Immunol Methods 95:57 Google Scholar
  94. 94.
    Morgan E, Varro R, Sepulveda H et al. (2004) Clin Immunol 110:252 Google Scholar
  95. 95.
    Nolan JP, Sklar LA (2002) Trends Biotechnol 20:9 Google Scholar
  96. 96.
    Nolan JP, Mandy F (2006) Cytometry A 69:318 Google Scholar
  97. 97.
    Davey HM (2002) Methods Cell Sci 24:91 Google Scholar
  98. 98.
    Shapiro HM (2000) J Microbiol Methods 42:3 Google Scholar
  99. 99.
    Winson MK, Davey HM (2000) Methods 21:231 Google Scholar
  100. 100.
    Fernandez LA, Hatch EW, Armann B et al. (2005) Transplantation 80:729 Google Scholar
  101. 101.
    Furlong EE, Profitt D, Scott MP (2001) Nat Biotechnol 19:153 Google Scholar
  102. 102.
    Huh D, Gu W, Kamotani Y et al. (2005) Physiol Meas 26:R73 Google Scholar
  103. 103.
    Chan SD, Luedke G, Valer M et al. (2003) Cytometry A 55:119 Google Scholar
  104. 104.
    Palkova Z, Vachova L, Valer M et al. (2004) Cytometry A 59:246 Google Scholar
  105. 105.
    Lancaster C, Kokoris M, Nabavi M et al. (2005) Methods 37:120 Google Scholar
  106. 106.
    Wolff A, Perch-Nielsen IR, Larsen UD et al. (2003) Lab Chip 3:22 Google Scholar
  107. 107.
    Cheung K, Gawad S, Renaud P (2005) Cytometry A 65:124 Google Scholar
  108. 108.
    Levin A, Brubaker G, Shao JS et al. (1996) Int J STD AIDS 7:288 Google Scholar
  109. 109.
    Young NL, Ponglertnapakorn P, Shaffer N et al. (1997) Clin Diagn Lab Immunol 4:783 Google Scholar
  110. 110.
    Gonzalo C, Boixo JC, Carriedo JA et al. (2004) J Dairy Sci 87:3623 CrossRefGoogle Scholar
  111. 111.
    Gunasekera TS, Veal DA, Attfield PV (2003) Int J Food Microbiol 85:269 Google Scholar
  112. 112.
    Christensen P, Knudsen DB, Wachmann H et al. (2004) Theriogenology 62:1218 Google Scholar
  113. 113.
    Christensen P, Stenvang JP, Godfrey WL (2004) J Androl 25:255 Google Scholar
  114. 114.
    Garner DL (2001) J Androl 22:519 Google Scholar
  115. 115.
    Johnson LA (2000) Anim Reprod Sci 60-61:93 Google Scholar
  116. 116.
    Dubelaar GB, Geerders PJ, Jonker RR (2004) J Environ Monit 6:946 Google Scholar
  117. 117.
    Dubelaar GB, Gerritzen PL, Beeker AE et al. (1999) Cytometry 37:247 Google Scholar

Copyright information

© Springer-Verlag Berlin Heidelberg 2008

Authors and Affiliations

  1. 1.BD BiosciencesSan JoseUSA

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