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Resonance Energy Transfer in Biophysics: Formalisms and Application to Membrane Model Systems

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Fluorescence of Supermolecules, Polymers, and Nanosystems

Part of the book series: Springer Series on Fluorescence ((SS FLUOR,volume 4))

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Abstract

Useful models for resonance energy transfer (RET) are reviewed for several geometries relevant tomembranes (planar, bilayer, multilayer) and uniform donor and acceptor probe distribution. Extensions fornon-uniform distribution of fluorophores are presented and discussed. Selected examples of quantitativeapplications of RET to these systems are described. It is illustrated how information about lipid phaseseparation (phase composition, domain size, partition coefficients, kinetics of lipid demixing) lipid–peptide(domain induction/membrane aggregation), lipid–protein (lipid selectivity in the annular region) andlipid–DNA (lipoplex structural characterization) interactions can be recovered from time-resolvedRET data.

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Author information

Authors and Affiliations

  1. Faculdade de Farmácia, Universidade de Coimbra, Rua do Norte, 3000-295, Coimbra, Portugal

    Luís M.S. Loura

  2. Centro de Química de Évora, Rua Romão Ramalho 59, 7000-671, Évora, Portugal

    Luís M.S. Loura

  3. Centro de Química-Física Molecular, Instituto Superior Técnico, Av. Rovisco Pais, 1049-001, Lisboa, Portugal

    Manuel Prieto

Authors
  1. Luís M.S. Loura
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  2. Manuel Prieto
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Correspondence to Luís M.S. Loura .

Editor information

M. N. Berberan-Santos

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© 2007 Springer-Verlag Berlin Heidelberg

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Loura, L.M., Prieto, M. (2007). Resonance Energy Transfer in Biophysics: Formalisms and Application to Membrane Model Systems. In: Berberan-Santos, M.N. (eds) Fluorescence of Supermolecules, Polymers, and Nanosystems. Springer Series on Fluorescence, vol 4. Springer, Berlin, Heidelberg. https://doi.org/10.1007/4243_2007_016

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