Skip to main content

Evaluation of Size and Zeta Potential of DNA/Carrier Complexes

  • Chapter
Non-viral Gene Therapy

This is a preview of subscription content, log in via an institution to check access.

Access this chapter

Chapter
USD 29.95
Price excludes VAT (USA)
  • Available as PDF
  • Read on any device
  • Instant download
  • Own it forever
eBook
USD 129.00
Price excludes VAT (USA)
  • Available as PDF
  • Read on any device
  • Instant download
  • Own it forever
Softcover Book
USD 169.99
Price excludes VAT (USA)
  • Compact, lightweight edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info
Hardcover Book
USD 169.99
Price excludes VAT (USA)
  • Durable hardcover edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info

Tax calculation will be finalised at checkout

Purchases are for personal use only

Institutional subscriptions

Preview

Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.

References

  • Bartlett GR (1959) Phosphorus assay in column chromatography. J. Biol Chem 234:466–468

    CAS  PubMed  Google Scholar 

  • Kichler A, Zauner W, Ogris M, Wagner E. (1998) Influence of the DNA complexation medium on the transfection efficiency of lipospermine/DNA particles. Gene Ther 5:855–860

    Article  CAS  PubMed  Google Scholar 

  • Li S, Rizzo MA, Bhattacharya S, Huang L (1998) Characterization of cationic lipidprotamine-DNA (LPD) complexes for intravenous gene delivery. Gene Ther 5:930–937

    CAS  PubMed  Google Scholar 

  • Morimoto K, Nishikawa M, Kawakami S, Nakano T, Hattori Y, Fumoto S, Yamashita F, Hashida M (2003) Molecular weight-dependent gene transfection activity of unmodi-fied and galactosylated polyethyleneimine on hepatoma cells and mouse liver. Mol Ther 7:254–261

    Article  CAS  PubMed  Google Scholar 

  • Niidome T, Huang L (2002) Gene therapy progress and prospects: nonviral vectors. Gene Ther 9:1647–1652

    Article  CAS  PubMed  Google Scholar 

  • Oh YK, Suh D, Kim JM, Choi HG, Shin K, Ko JJ (2002) Polyethylenimine-mediated cellular uptake, nucleus trafficking and expression of cytokine plasmid DNA Gene Ther. 20029:1627–1632

    Google Scholar 

  • Ogris M, Brunner S, Schuller S, Kircheis R, Wagner E. (1999) PEGylated DNA/transferrin-PEI complexes: reduced interaction with blood components, extended circulation in blood and potential for systemic gene delivery. Gene Ther 6:595–605

    Article  CAS  PubMed  Google Scholar 

  • Ogris M, Steinlein P, Kursa M, Mechtler K, Kircheis R, Wagner E. (1998) The size of DNA/transferrin-PEI complexes is an important factor for gene expression in cultured cells. Gene Ther 5:1425–1433

    Article  CAS  PubMed  Google Scholar 

  • Ross PC, Hui SW (1999) Lipoplex size is a major determinant of in vitro lipofection efficacy. Gene Ther 6:651–659

    Article  CAS  PubMed  Google Scholar 

  • Sakurai F, Inoue R, Nishino Y, Okuda A, Matsumoto O, Taga T, Yamashita F, Takakura Y, Hashida M (2000) Effect of DNA/liposome mixing ratio on the physicochemical characteristics, cellular uptake and intracellular trafficking of plasmid DNA/cationic liposome complexes and subsequent gene expression. J Control Release 66:255–69

    Article  CAS  PubMed  Google Scholar 

  • Sakurai F, Nishioka T, Saito H, Baba T, Okuda A, Matsumoto O, Taga T, Yamashita F, Takakura Y, Hashida M. (2001) Interaction between DNA-cationic liposome complexes and erythrocytes is an important factor in systemic gene transfer via the intravenous route in mice: the role of the neutral helper lipid. Gene Ther 8(9):677–686

    Article  CAS  PubMed  Google Scholar 

  • Sakurai F, Nishioka T, Yamashita F, Takakura Y, Hashida M. (2001) Effects of erythrocytes and serum proteins on lung accumulation of lipoplexes containing cholesterol or DOPE as a helper lipid in the single-pass rat lung perfusion system. Eur J Pharm Biopharm 52:165–72

    CAS  PubMed  Google Scholar 

  • Templeton NS, Lasic DD, Frederik PM, Strey HH, Roberts DD, Pavlakis GN (1997) Improved DNA: liposome complexes for increased systemic delivery and gene expression. Nat Biotechnol. 15:647–652

    Article  CAS  PubMed  Google Scholar 

Download references

Author information

Authors and Affiliations

Authors

Editor information

Editors and Affiliations

Rights and permissions

Reprints and permissions

Copyright information

© 2005 Springer-Verlag Tokyo

About this chapter

Cite this chapter

Hattori, Y., Hashida, M. (2005). Evaluation of Size and Zeta Potential of DNA/Carrier Complexes. In: Taira, K., Kataoka, K., Niidome, T. (eds) Non-viral Gene Therapy. Springer, Tokyo. https://doi.org/10.1007/4-431-27879-6_22

Download citation

Publish with us

Policies and ethics