Summary
With regards to the therapy for end-stage failing hearts, the heart transplantation is the only effective treatment but is still limited by donor shortage and chronic rejection. Therefore, a novel strategy for myocardial regeneration is desired. Cell transplantation, a new approach for restoring impaired hearts still has several problems. Especially, autologous skeletal myoblasts (Skm) transplantation (Ctx) by injection has been clinically applied. However, its optimal effect and stable delivery methods are undetermined. Tissue implantation provides stable cell delivery with maintained inter-cellular communication and extracellular matrix. We hypothesize that the Skm tissue cardiomyoplasty might be more advantageous in regenerating the impaired heart. LAD ligated hearts (2 weeks) received Skm Ctx either by injection (MI; n=9) or by implanting two engineered monolayer myoblasts sheets (MS; n=9) or non-cellular therapy (Control=C; n=10). After 8 weeks, higher improvement of ejection fraction (%) was measured in MS compared to that of the other groups. Histological comparison revealed greater cellularity and abundant widespread neo-capillaries with a noticeably uniformly thickened wall in MS only. These results demonstrated that engineered skeletal myoblasts sheets regenerated the impaired myocardium, suggesting a promising therapy for severe heart failure.
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© 2005 Springer-Verlag Tokyo
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Sawa, Y., Memon, I., Matsuda, H. (2005). Myocardial Regeneration Therapy with Tissue Implantation of Autologous Myoblast Sheets for Severe Impaired Heart Failure. In: Mori, H., Matsuda, H. (eds) Cardiovascular Regeneration Therapies Using Tissue Engineering Approaches. Springer, Tokyo. https://doi.org/10.1007/4-431-27378-6_5
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DOI: https://doi.org/10.1007/4-431-27378-6_5
Publisher Name: Springer, Tokyo
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