Summary
In an attempt to evaluate local architectural changes in oral epithelial premalignancy and malignancy, a quantitative method to analyse spatial cell arrangement as observed in 2D histological sections was developed based on mathematical morphology and graph theory. In total, 441 images (x20) of oral epithelium belonging to three diagnostic classes of interest (normal, dysplastic and neoplastic lesions) were assembled into collages for analysis. Epithelial cell nuclei markers were created from Haematoxylin and Eosin stained sections using colour deconvolution and morphological greyscale reconstructions. The epithelial tissue compartment was partitioned (using a digital watershed algorithm) into exclusive domains according to nuclei positions to approach the theoretical cell extents. The spatial arrangement of these “cells” was then analysed in circular neighbourhoods of two sizes where four types of constrained graph networks (minimal spanning tree, relative neighbour graph, Gabriel graph and Delaunay triangulation) were constructed over the cell centroids. From these networks a total of 29 statistical properties were recorded. The statistical analysis of the network data indicated that unbiased and reproducible quantification of tissue architectural features is feasible and may provide valuable morphological information for diagnostic purposes and tissue characterization.
Access this chapter
Tax calculation will be finalised at checkout
Purchases are for personal use only
Preview
Unable to display preview. Download preview PDF.
References
Ferlay J, Bray F, Pisani P, Parkin DM. GLOBOCAN 2000: Cancer incidence, mortality and prevalence worldwide, Version 1.0. IARC CancerBase No. 5. Lyon, IARC Press, 2001.
Landini G, Rippin JW. Fractal dimension of the epithelial connective tissue interfaces in premalignant and malignant lesions of the floor of the mouth. Anal Quant Cytol Histo 1993; 15(2) 144–149.
Landini G, Rippin JW. How important is tumour shape? Quantification of the epithelial-connective tissue interface in oral lesions using local connected fractal dimension analysis. J Pathol 1996; 179:210–217.
Landini G. Pattern complexity in organogenesis and carcinogenesis. In: Fractals in Biology and Medicine III, (GA Losa, D Merlini, TF Nonnenmacher, ER Weibel eds.), 3–13, Birkhäuser Verlag, Basel, 2002.
Abu Eid R, Landini G. Quantification of the global and local complexity of the epithelial-connective tissue interface of normal, dysplastic and neoplastic oral mucosae using digital imaging. Path Res Prac 2003; 199(7):475–482.
Marcelpoil R, Davoine F, Robert-Nicaud M. (1994) Cellular sociology: parametrization of spatial relationships based on Voronoi diagram and Ulam trees. In: Nonnenmacher, T. F., Losa, G. A., Weibel, E. R. (Eds.), Fractals in Biology and Medicine, 201–209. Birkhäuser Verlag, Basel.
Sudbø J, Bankfalvi A, Bryne M, Marcelpoil R, Boysen M, Piffko J, Hemmer J, Kraft K, Reith A. Prognostic value of graph theory-based tissue architecture analysis in carcinomas of the tongue. Lab Invest 2000; 80(12):1881–1889.
Landini G, Othman IE. Estimation of tissue layer level by sequential morphological reconstruction. J Microsc 2003; 209(2):118–125.
Ruifrok AC, Johnston DA. Quantification of histological staining by color deconvolution. Anal Quant Cytol Histol 2001; 23:291–299.
Vincent L, Soille P. (1991) Watersheds in digital spaces: an efficient algorithm based on immersion simulations. IEEE Trans Patt Anal Machine Intell 1991; 13(6):583–598.
Bhattacharya BK, Poulsen RS, Toussaint GT. Application of proximity graphs to editing nearest neighbor decision rule. International Symposium on Information Theory, Santa Monica, 1981.
Toussaint GT. The relative neighbourhood graph of a finite planar set. Pattern Recogn 1980;12:261–268.
Stoyan D, Kendall WS, Mecke J. Stochastic Geometry and its Applications. 2nd edition, Wiley & Sons, Chichester, 1995.
Pindborg JJ, Reibel J, Holmstrup P. Subjectivity in evaluating oral epithelial dysplasia, carcinoma in situ and initial carcinoma. J Oral Path 1985; 14:698–708.
Warnakulasuriya S. Histological grading of oral epithelial dysplasia: revisited. J Pathol. 2001;194:294–297.
Bosman FT. Dysplasia classification: pathology in disgrace? J Pathol 2001; 194: 143–144.
Fleming KA, Evidence-based pathology. J Pathol 1996; 179(2):127–128.
Cross SS, Burym JP, Silcocks PB, Stephenson TJ, Cotton DWK. Fractal geometric analysis of colorectal polyps. J Pathol 1994; 172:317–323.
Morris JA. Information and observer disagreement in histopathology. Histopathology 1994; 25: 123–128.
Author information
Authors and Affiliations
Editor information
Editors and Affiliations
Rights and permissions
Copyright information
© 2005 Birkhäuser Verlag Basel
About this paper
Cite this paper
Landini, G., Othman, I. (2005). Quantification of Local Architecture Changes Associated with Neoplastic Progression in Oral Epithelium using Graph Theory. In: Losa, G.A., Merlini, D., Nonnenmacher, T.F., Weibel, E.R. (eds) Fractals in Biology and Medicine. Mathematics and Biosciences in Interaction. Birkhäuser Basel. https://doi.org/10.1007/3-7643-7412-8_18
Download citation
DOI: https://doi.org/10.1007/3-7643-7412-8_18
Publisher Name: Birkhäuser Basel
Print ISBN: 978-3-7643-7172-2
Online ISBN: 978-3-7643-7412-9
eBook Packages: Biomedical and Life SciencesBiomedical and Life Sciences (R0)