On-line and Off-line Monitoring of the Production of Cephalosporin C by Acremonium chrysogenum

  • G. Seidel
  • C. Tollnick
  • M. Beyer
  • K. Schügerl
Part of the Advances in Biochemical Engineering/Biotechnology book series (ABE, volume 66)


Process monitoring of cephalosporin C formation by Acremonium chrysogenum in laboratory investigations is considered. The goal of these investigations is the identification of bottlenecks in the biosynthesis and the improvement of the process performance. Based on reports of other research groups and own experience the key parameters were selected, which influence the process performance. They are: dissolved oxygen and pH values. In addition the concentrations of biomass, DNA, glucose and reducing sugars (glucose, maltose, maltotriose and oligosaccharides), methionine, other nitrogen sources (ammonium ion, other amino acids), organic acids, phosphate, sulfate, dissolved organic carbon, proteins, product and precursors in the cell free cultivation medium are monitored. In addition the intracellular concentrations of RNA, DNA, proteins, amino acids as well as the activities of the enzymes of the biosynthesis of cephalosporin C are determined. The influence of these parameters on the biosynthesis is discussed.


Cephalosporin C Acremonium chrysogenum Extracellular components Intracellular components Enzyme activities of biosynthesis Bottleneck of biosynthesis 


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Copyright information

© Springer-Verlag Berlin Heidelberg 1999

Authors and Affiliations

  • G. Seidel
    • 1
  • C. Tollnick
    • 1
  • M. Beyer
    • 1
  • K. Schügerl
    • 1
  1. 1.Institut für Technische Chemie der Universität HannoverHannoverFRG

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