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Production of Core and Virus-Like Particles with Baculovirus Infected Insect Cells

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Book cover Tools and Applications of Biochemical Engineering Science

Part of the book series: Advances in Biochemical Engineering/Biotechnology ((ABE,volume 74))

Abstract

In this paper the fundamental aspects of process development for the production of core and virus-like particles with baculovirus infected insect cells are reviewed. The issues addressed include: particle formation and monomer composition, chemical and physical conditions for optimal cell growth, baculovirus replication and product expression, multiplicity of infection strategy, and scale-up of the process. Study of the differences in the metabolic requirements of infected and non-infected cells is necessary for high cell density processes. In the bioreactor, the specific oxygen uptake rate (OURsp) plays a central role in process scale-up, leading to the specification of the bioreactor operational parameters. Shear stress can also be an important variable for bioreactor operation due to its influence on cell growth and product expression.

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Author information

Authors and Affiliations

  1. Instituto de Biologia Experimental e Tecnológica/Instituto de Tecnologia Química e Biológica IBET/ITQB, Apartado 12, P-2781-901, Oeiras, Portugal

    Luis Maranga, Pedro E. Cruz, John G. Aunins & Manuel J. T. Carrondo

  2. ECBio, Ltd, Taguspark, Núcleo Central, 332, P-2780-920, Porto Salvo, Portugal

    Pedro E. Cruz

  3. Fermentation and Cell Culture, Vaccine Bioprocess R&D, Merck Research Laboratories, Merck & Co., Inc., West Point, Pennsylvania, USA

    John G. Aunins

  4. Laboratório de Engenharia Bioquímica, Faculdade de Ciências e Tecnologia, Universidade Nova de Lisboa, P-2825-114, Monte da Caparica, Portugal

    Manuel J. T. Carrondo

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  1. Luis Maranga
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  2. Pedro E. Cruz
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  3. John G. Aunins
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  4. Manuel J. T. Carrondo
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Editor information

Editors and Affiliations

  1. Institute of Technical Chemistry, University of Hannover, Callinstraße 3, 30167, Hannover, Germany

    K. Schügerl

  2. GBF Biochemical Engineering Division, Mascheroder Weg 1, 38124, Braunschweig, Germany

    A.-P. Zeng

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Dedicated to Prof. Dr. Wolf-Dieter Deckwer on the occasion of his 60th birthday

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Maranga, L., Cruz, P.E., Aunins, J.G., Carrondo, M.J.T. (2002). Production of Core and Virus-Like Particles with Baculovirus Infected Insect Cells. In: Schügerl, K., et al. Tools and Applications of Biochemical Engineering Science. Advances in Biochemical Engineering/Biotechnology, vol 74. Springer, Berlin, Heidelberg. https://doi.org/10.1007/3-540-45736-4_9

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  • DOI: https://doi.org/10.1007/3-540-45736-4_9

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