Abstract
Perfusion cultures of animal cells have several advantages over batch or fed-batch cultures. They give, for instance, higher productivities and a consistent product quality, and allow steady state operation and better cell physiology control. However, one of the main aspects limiting performance and scale-up of perfusion processes is the need for an adequate cell retention device. The devices currently in use for stirred perfusion bioreactors are continuous centrifuges, tangential flow membrane filters, dynamic filters, spin-filters, ultrasonic and di- electrophoretic separators, gravity settlers and, more recently, hydrocyclones. The advantages and disadvantages of each of these methods will be discussed.
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Dedicated to Prof. Dr. Wolf-Dieter Deckwer on the occasion of his 60th birthday
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Castilho, L.R., Medronho, R.A. (2002). Cell Retention Devices for Suspended-Cell Perfusion Cultures. In: Schügerl, K., et al. Tools and Applications of Biochemical Engineering Science. Advances in Biochemical Engineering/Biotechnology, vol 74. Springer, Berlin, Heidelberg. https://doi.org/10.1007/3-540-45736-4_7
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