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High-Density GeneChip Oligonucleotide Probe Arrays

  • Glenn H. McGall
  • Fred C. Christians
Chapter
Part of the Advances in Biochemical Engineering/Biotechnology book series (ABE, volume 77)

Abstract

High-density DNA probe arrays provide a highly parallel approach to nucleic acid sequence analysis that is transforming gene-based biomedical research. Photolithographic DNA synthesis has enabled the large-scale production of GeneChip probe arrays containing hundreds of thousands of oligonucleotide sequences on a glass “chip” about 1.5 cm2 in size. The manufacturing process integrates solid-phase photochemical oligonucleotide synthesis with lithographic techniques similar to those used in the microelectronics industry. Due to their very high information content, GeneChip probe arrays are finding widespread use in the hybridization-based detection and analysis of mutations and polymorphisms (“genotyping”), and in a wide range of gene expression studies.

Keywords

GeneChip array Oligonucleotide probe array Photolithography Gene expression monitoring Genotyping 

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Copyright information

© Springer-Verlag Berlin Heidelberg 2002

Authors and Affiliations

  • Glenn H. McGall
    • 1
  • Fred C. Christians
    • 2
  1. 1.Affymetrix, Inc.Santa ClaraUSA
  2. 2.Affymetrix, Inc.Santa ClaraUSA

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