Abstract
It is well known, that organism of animal, consisting of many billions cells, is formed by consequent divisions of the only cell - zygote. In so doing, embryo cells are permanently communicating by means of biochemical signals. As a result, proper genes were being activated at proper time in proper cells of the embryo.
Modern confocal microscopes being equipped by lasers and computers give possibility to trace-through the cell fate of early embryo for such a classical model object, as fruit fly Drosophila melanogaster. By this approach, it is possible to retrace the detailed dynamics of activity of genes-controllers of development with the resolution on the level of individual nuclei for each of 4-6 thousand cells, composing early fly embryo. The final result of this analysis will be the quantitative atlas of Drosophila genes action (expression): http://www.iephb.nw.ru/ spirov/atlas. To achieve this aim we need to receive statistically authentic summary picture of detailed pattern dynamics proceeding from a large number of scanned embryos. This presupposes the elaboration of the methods of preprocessing, elastic deformation, registration and interpolation of the confocal-microscopy images of embryos.
For this purpose we apply modern heuristic methods of optimization to the processing of our images. Namely classic GA approach is used for finding a suitable elastic deformation, for registering the images and for finding a Fourier interpolation of concentration (gene-expression) surfaces. All GA programs considered are the developments of “evolution strategies program” from EO-0.8.5 C++ library (Merelo).
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Spirov, A.V., Timakin, D.L., Reinitz, J., Kosman, D. (2001). Using of Evolutionary Computations in Image Processing for Quantitative Atlas of Drosophila Genes Expression. In: Boers, E.J.W. (eds) Applications of Evolutionary Computing. EvoWorkshops 2001. Lecture Notes in Computer Science, vol 2037. Springer, Berlin, Heidelberg. https://doi.org/10.1007/3-540-45365-2_39
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DOI: https://doi.org/10.1007/3-540-45365-2_39
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