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Protein Spot Detection by Symmetry Derivatives of Gaussians

  • Martin Persson
  • Josef Bigun
Conference paper
Part of the Lecture Notes in Computer Science book series (LNCS, volume 2749)

Abstract

Two-dimensional gel electrophoresis is the preferred method for simultaneously separating and visualising thousands of proteins. An important part of the computer aided analysis of the proteome is the ability to automatically detect, identify, and quantify the proteins by means of automatic Image processing. We present a fast and sensitive method for protein spot detection using the Circular Symmetry Tensor. It is based upon the work of Bigun on Symmetry derivatives of Gaussians.

Keywords

Protein Spot Polar Coordinate System Orientation Tensor Circular Symmetry Conjugate Symmetry 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

References

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    O’Farrell, P.H., High resolution two-dimensional electrophoresis of proteins, J. Biol. Chem. 250:4007–4021, 1975Google Scholar
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    Bigün, J., Bigün T., Symmetry derivatives of Gaussians illustrated by cross tracking, Research Report IDE-0131, 2001Google Scholar
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    Bigün, J., Optimal orientation detection of linear symmetry, IEEE Computer Society Press, Washington, DC, pages 433–438, 1987, In First International Conference on Computer Vision, ICCV (London).Google Scholar
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    Bigün, J., A structure feature for some image processing applications based on spiral faunctions, Computer Vision, Graphics, and Image Processing, 51(2):166–194, 1990CrossRefGoogle Scholar
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    Raman, B., Cheung, A., Marten, M. R., Quantitative comparison and evaluation of two commercially available, two-dimensional electrophoresis image analysis software packages, Z3 and Melanie, Electrophoresis 23:2194–2202Google Scholar
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    http://userpages.umbc.edu/~braman1/proteome_index.htmlGoogle Scholar

Copyright information

© Springer-Verlag Berlin Heidelberg 2003

Authors and Affiliations

  • Martin Persson
    • 1
  • Josef Bigun
    • 1
  1. 1.School of Information Science, Computer and Electrical EngineeringHalmstad UniversityHalmstadSweden

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