Advances in Enzyme Technology — UK Contributions

  • John M. Woodley
Part of the Advances in Biochemical Engineering/Biotechnology book series (ABE, volume 70)


Enzyme technology has been a recognised part of bioprocess engineering since its inception in the 1950s and 1960s. In this article the early history of enzyme technology is discussed and the subsequent developments in enzyme isolation, enzyme modification and process technology are described. These creative developments have put enzyme technology in a position of huge potential to contribute to environmentally compatible and cost effective means of industrial chemical synthesis. Recent developments in protein modification to produce designer enzymes are leading a new wave of enzyme application.


Enzyme isolation Enzyme technology Enzyme immobilisation Protein engineering 


Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.


  1. 1.
    Faber K (1995) biotransformations in Organic Chemistry, Springer, Berlin Heidelberg New YorkGoogle Scholar
  2. 2.
    Meyer H-P, Kiener A, Imwinkelvied R, Shaw N (1997) Chimia 51:287Google Scholar
  3. 3.
    Peterson DH, Murray HC, Eppstein SH, Reineke LM, Weintaub A, Meister PD, Leigh HM (1952) J Am Chem Soc 74:5933CrossRefGoogle Scholar
  4. 4.
    Kay G (1968) Process Biochem 3(8):36Google Scholar
  5. 5.
    Manecke G (1962) Pure Appl Chem 4:507CrossRefGoogle Scholar
  6. 6.
    Lilly MD, Money C, Hornby, WE, Crook EM (1965) Biochem J 95:45Google Scholar
  7. 7.
    Bar-Eli A, Katchalski E (1963) J Biol Chem 238:1690Google Scholar
  8. 8.
    Mosbach K, Mosbach R (1966) Acta Chem Scand 20:2807CrossRefGoogle Scholar
  9. 9.
    Mosbach K (1970) Acta Chem Scand 24:2084CrossRefGoogle Scholar
  10. 10.
    Sundaram PV, Pye EK, Chang TMS, Edwards VH, Humphrey AE, Kaplan NO, Katchalski E, Levin Y, Lilly MD, Manecke G, Mosbach K, Patchornik A, Porath J, Weethall HH, Wingard LB (1972) Biotechnol Bioeng Symp 3:15Google Scholar
  11. 11.
    Reisenberg D, Menzel K, Schulz V, Scumann K, Vieth G, Zuber G, Knorre AW (1990) Appl Microbiol Biotechnol 34:77Google Scholar
  12. 12.
    Reisenberg D, Schulz V, Knorre WA, Pohl HD, Karz D, Sanders EA, Ross A, Deckwer WD (1991) J Biotechnol 20:17CrossRefGoogle Scholar
  13. 13.
    Hobbs GR, Lilly MD, Turner NJ, Ward JM, Willetts AJ, Woodley JM (1993) J Chem Soc Perkin Trans I(2):165CrossRefGoogle Scholar
  14. 14.
    Lilly MD, Chauhan R, French C, Gyamerah M, Hobbs GR, Humphrey A, Isupov M, Littlechild JA, Mitra RK, Morris KG, Rupprecht M, Turner NJ, Ward JM, Willetts AJ, Woodley JM (1996) Ann NY Acad Sci 782:513CrossRefGoogle Scholar
  15. 15.
    Mahmoudian M, Noble D, Drake CS, Middleton RF, Montgomery DS, Piercey JE, Ramlakhan D, Todd M, Dawson MJ (1997) Enzyme Microb Technol 20:393CrossRefGoogle Scholar
  16. 16.
    Hetherington PJ, Follows M, Dunnill P, Lilly MD (1971) Trans Instn Chem Engrs 49:142Google Scholar
  17. 17.
    Dunnill P, Lilly MD (1972) Biotechnol Bioeng Symp 3:97Google Scholar
  18. 18.
    Buckland BC, Richmond W, Dunnill P, Lilly MD (1974) The large-scale isolation of intracellular microbial enzymes: cholesterol oxidase from Nocardia. In: Spencer B (ed) Industrial Aspects of Biochemistry. FEBS, Amsterdam, p 65Google Scholar
  19. 19.
    Thomas CR, Dunnill P (1979) Biotechnol Bioeng 21:2279CrossRefGoogle Scholar
  20. 20.
    Talboys BL, Dunnill P (1985) Biotechnol Bioeng 27:1730CrossRefGoogle Scholar
  21. 21.
    Lilly MD, Dunnill P (1971) Process Biochem 6(8):29Google Scholar
  22. 22.
    Goldstein L, Levin Y, Katchalski E (1964) Biochemistry 3:1913CrossRefGoogle Scholar
  23. 23.
    Tosa T, Mori T, Fuse N, Chibata I (1969) Agr Biol Chem 33:1047Google Scholar
  24. 24.
    Lilly MD (1979) Dechema Monograph 82:165Google Scholar
  25. 25.
    Lilly MD (1994) Chem Eng Sci 49:151CrossRefGoogle Scholar
  26. 26.
    Lilly MD, Sharp AK (1968) The Chemical Engineer 215:CE12Google Scholar
  27. 27.
    Self DA, Kay G, Lilly MD (1969) Biotechnol Bioeng 11:283CrossRefGoogle Scholar
  28. 28.
    Warburton D, Dunnill P, Lilly MD (1973) Biotechnol Bioeng 15:13CrossRefGoogle Scholar
  29. 29.
    Savidge TA (1984) Enzymatic conversions used in the production of penicillins and cephalosporins. In: Vandamme E (ed) Biotechnology of Industrial Antibiotics. Marcel Dekker, New York, p 171Google Scholar
  30. 30.
    Tischer W, Kasche V (1999) Trends Biotechnol 17:326CrossRefGoogle Scholar
  31. 31.
    Woodley JM, Lilly MD (1994) Biotransformation reactor selection and operation. In: Cabral JMS, Best D, Boross L, Tramper J (eds) Applied Biocatalysis. Harwood Academic, Chur, p371Google Scholar
  32. 32.
    Lilly MD, Dunnill P (1972) Biotech Bioeng Symp 3:221Google Scholar
  33. 33.
    Lieberman RB, Ollis DF (1975) Biotechnol Bioeng 17:1401CrossRefGoogle Scholar
  34. 34.
    Sada E, Katoh S, Shioza M, Fukui T (1981) Biotechnol Bioeng 23:2561CrossRefGoogle Scholar
  35. 35.
    Cabral JMS, Tramper J (1984) Bioreactor design. In: Cabral JMS, Best D, Boross L, Tramper J (eds) Applied Biocatalysis, Harwood Academic, Chur, p 333Google Scholar
  36. 36.
    Hill AC (1898) J Chem Soc 73:634Google Scholar
  37. 37.
    Kastle JC, Loenhart AS (1900) Am Chem J 24:491Google Scholar
  38. 38.
    Sym EA (1936) Biochem J 30:609Google Scholar
  39. 39.
    Cremonesi P, Carrea G, Ferrara L, Antonini E (1975) Biotechnol Bioeng 17:1101CrossRefGoogle Scholar
  40. 40.
    Buckland BC, Dunnill P, Lilly MD (1975) Biotechnol Bioeng 17:815CrossRefGoogle Scholar
  41. 41.
    Lilly MD (1982) J Chem Tech Biotechnol 32:162Google Scholar
  42. 42.
    Lilly MD (1983) Phil Trans R Soc London 300:391CrossRefGoogle Scholar
  43. 43.
    Carrea G (1984) Trends Biotechnol 2:102CrossRefGoogle Scholar
  44. 44.
    Zaks A, Klibanov AM (1984) Science 224:1249CrossRefGoogle Scholar
  45. 45.
    Zaks A, Klibanov AM (1985) Proc Natl Acad Sci USA 82:3192CrossRefGoogle Scholar
  46. 46.
    Macrae AR (1983) J Am Oil Chem Soc 60:291CrossRefGoogle Scholar
  47. 47.
    Halling PJ, Valivety RH (1992) Progress Biotechnol 8:13Google Scholar
  48. 48.
    Brink LES, Tramper J (1985) Biotechnol Bioeng 27:1258CrossRefGoogle Scholar
  49. 49.
    Laane C, Boeren S, Vos K (1985) Trends Biotechnol 3:251CrossRefGoogle Scholar
  50. 50.
    Lilly MD, Dervakos G, Woodley JM (1990) Two-liquid phase biocatalysis: choice of phase ratio. In: Copping LG, Martin RE, Pickett JE, Bucke C, Bunch AW (eds) Opportunities in Biotransformation, Elsevier, London, p 5Google Scholar
  51. 51.
    Woodley JM, Lilly MD (1992) Progress Biotechnol 8:147Google Scholar
  52. 52.
    Gibson DT, Hensley M, Yoshoka H, Mabry TJ (1970) Biochemistry 9:1626CrossRefGoogle Scholar
  53. 53.
    Taylor SC, Brown S (1986) Perf Chem:20Google Scholar
  54. 54.
    Collins AM, Woodley JM, Liddell JM (1995) J Ind Microbiol 14:382CrossRefGoogle Scholar
  55. 55.
    Schmid A, Kollmer A, Mathys RG, Witholt B (1998) Extremophiles 2:249CrossRefGoogle Scholar
  56. 56.
    Leon R, Fernandes P, Pinheiro HM, Cabral JMS (1998) Enzyme Microb Technol 23:483CrossRefGoogle Scholar
  57. 57.
    Roffler SR, Blanch HW, Wilke CR (1984) Trends Biotechnol 2:129CrossRefGoogle Scholar
  58. 58.
    Freeman A, Woodley JM, Lilly MD (1993) Bio/Technology 11:1007CrossRefGoogle Scholar
  59. 59.
    Lye GJ, Woodley JM (1999) Trends Biotechnol 17:395CrossRefGoogle Scholar
  60. 60.
    Vicenzi JT, Zmijewski MJ, Reinhard MR, Landen BE, Muth WL, Marler PG (1997) Enzyme Microb Technol 20:494CrossRefGoogle Scholar
  61. 61.
    Lilly MD, Woodley JM (1996) J Ind Microbiol 17:24CrossRefGoogle Scholar
  62. 62.
    Woodley JM, Titchener-Hooker NJ (1996) Bioprocess Engng 14:263CrossRefGoogle Scholar
  63. 63.
    Blayer S, Woodley JM, Lilly MD, Dawson MJ (1996) Biotechnol Progr 12:758CrossRefGoogle Scholar
  64. 64.
    Affholter J, Arnold FH (1999) Chemtech 29:34Google Scholar
  65. 65.
    Crameri A, Raillard SA, Bermudez E, Stemmer WP (1998) Nature 391:288CrossRefGoogle Scholar
  66. 66.
    Zhao H, Moore JC, Volkov AA, Arnold FH (1999) Methods for optimizing industrial enzymes by directed evolution. In: Demain AL, Davies JE (eds) Manual of Industrial Microbiology and Biotechnology, 2nd edn. ASM Press, Washington DC, p 597Google Scholar
  67. 67.
    Joo H, Lin Z, Arnold FH (1999) Nature 399:670CrossRefGoogle Scholar
  68. 68.
    Roberts GCK (1999) Chem Biol 6:R269CrossRefGoogle Scholar
  69. 69.
    McCoy M (1999) Chen Eng News 77:10Google Scholar
  70. 70.
    Drauz K, Waldmann H (eds) (1995) Handbook of Enzyme Catalysis in Organic Synthesis. VCH, WeinheimGoogle Scholar
  71. 71.
    Wong C-H, Halcomb RL, Ichikawa Y, Kajimoto T (1995) Angew Chem Int Ed 34:421Google Scholar
  72. 72.
    Kula M-R, Wandrey C (1987) Meth Enzymol 136:9CrossRefGoogle Scholar

Copyright information

© Springer-Verlag Berlin Heidelberg 2000

Authors and Affiliations

  • John M. Woodley
    • 1
  1. 1.The Advanced Centre for Biochemical Engineering, Department of Biochemical EngineeringUniversity College LondonLondonUK

Personalised recommendations