Abstract
The recent completion of the Pseudomonas Genome Project, in conjunction with the Pseudomonas Community Annotation Project (PseudoCAP) has fast-tracked our ability to apply the tools encompassed under the term ‘proteomics’ to this pathogen. Such global approaches will allow the research community to answer long-standing questions regarding the ability of Pseudomonas aeruginosa to survive diverse habitats, its high intrinsic resistance to antibiotics and its pathogenic nature towards humans. Proteomics provides an array of tools capable of confirming the expression of Open Reading Frames (ORF), the relative levels of their expression, the environmental conditions required for this expression and the sub-cellular location of the encoded gene-products. Since proteins are important cellular effectors, the biological questions we pose can be defined in terms of changes in protein expression detectable by separation to purity using two-dimensional gel electrophoresis (2-DGE) and relation to gene sequences via mass spectrometry. As such, we can compare strains with well-characterized phenotypic differences, growth under a variety of stresses, protein interactions and complexes and aid in defining proteins of unknown function. While the complete genome has only recently been finished, a number of studies have already utilized this information and examined various protein gene-products using proteomics. This review summarizes the application of proteomics to P. aeruginosa and highlights potential areas of future research, including overcoming the traditional technical limitations associated with 2-DGE. More focused approaches that target sub-cellular fractions (‘sub-proteomes’) prior to 2-DGE can provide further functional information. A review of current and previous proteomic projects on P. aeruginosa is presented, as well as theoretical considerations of the importance of sub-proteomic approaches to enhance these investigations. A review of current and previous proteomic projects on P. aeruginosa is presented, as well as theoretical considerations of the importance of sub-proteomic approaches to enhance these investigations.
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References
Lyczak JB, Cannon CL, Pier GB (2000) Microb Infect 2:1051
Bellido F, Hancock R (1993) In: Campa M, Bendinelli M, Friedman H (eds) Pseudomonas aeruginosa as an opportunistic pathogen. Plenum Press, New York, p 321
Britigan BE, Railsback M, Cox CD (1999) Infect Immun 67:1207
Meyer JM (2000) Arch Microbiol 174:135
DeVos D, De Chial M, Cochez C, Jansen S, Tummler B, Meyer JM, Cornelis P (2001) Arch Microbiol 175:384
Blumer C, Haas D (2000) Microbiology 146:2417
Galloway DP (1991) Mol Microbiol 5:2315
Iglewski BH, Kabat D (1975) Proc Natl Acad Sci USA 2:2284
Hornef MW, Roggenkamp A, Geiger AM, Hogardt M, Jacobi CA, Heesemann J (2000) Microb Pathog 29:329
Poole K (2001) Curr Opin Microbiol 4:500
Poole K, Tetro K, Zhao Q, Neshat S, Heinrichs DE, Bianco N (1996) Antimicrob Agents Chemother 40:2021
Srikumar R, Paul CJ, Poole K (2000) J Bacteriol 182:1410
Ziha-Zarifi I, Llanes C, Kohler T, Pechere JC, Plesiat P (1999) Antimicrob Agents Chemother 43:287
Hahn HP (1997) Gene 192:99
Comolli JC, Hauser AR, Waite L, Whitchurch CB, Mattick JS, Engel JN (1999) Infect Immun 67:3625
Poole K, Neshat S, Krebes K, Heinrichs DE (1993) J Bacteriol 175:4597
Folschweiller N, Schalk I J, Celia H, Kieffer B, Abdallah MA, Pattus F (2000) Mol Membr Biol 17:123
Stover CK, Pham XQ, Erwin AL, Mizoguchi SD, Warrener P, Hickey MJ, Brinkman FSL, Hurnagle WO, Kowalik DJ, Lagrou M, Garber RL, Goltry L, Tolentino E, Westbrock-Wadman S, Yuan Y, Brody LL, Coulter SN, Folger KR, Kas A, Larbig K, Lim R, Smith K, Spencer D, Wong GK-S, Wu Z, Paulsen IT, Reizer J, Saier MH, Hancock REW, Lory S, Olsen MV (2000) Nature 406:959
Croft L, Beatson SA, Whitchurch CB, Huang B, Blakeley RL, Mattick JS (2000) Microbiology 146:2351
Brinkmas FSL, Hancock REW, Stover CK (2000) Nature 406:933
Spiers AJ, Buckling A, Rainey PB (2000) Microbiology 146:2345
Harrington CA, Rosenow C, Retief J (2000) Curr Opin Microbiol 3:285
Ichikawa JK, Norris A, Bangera MG, Geiss GK, van’t Wout AB, Bumgarner RE, Lory S (2000) Proc Natl Acad Sci USA 97:9659
DeLisa MP, Wu CF, Wang L, Valdes JJ, Bentley WE (2001) J Bacteriol 183:5239
Schoolnik GK, Voskuil MI, Schnappinger D, Yildiz FH, Meibom K, Dolganov NA, Wilson MA, Chong KH (2001) Methods Enzymol 336:3
Gygi SP, Rochon Y, Franza BR, Aebersold R(1999) Mol Cell Biol 19:1720
Nouwens AS, Hopwood FG, Traini M, Williams KL, Walsh BJ (1999) In: Charlebois RL (ed) Organization of the prokaryotic genome. American Society for Microbiology, Washington DC, p 331
Cordwell SJ, Nouwens AS, Walsh BJ (2001) Proteomics 1:461
Washburn MP, Wolters D, Yates JR III (2001) Nature Biotechnol 19:242
Gygi SP, Rist B, Aebersold R (2000) Curr Opin Biotechnol 11:396
Smolka MB, Zhou H, Purkayastha S, Aebersold R (2001) Anal Biochem 297:25
Herbert B, Molloy M, Gooley A, Walsh B, Bryson W, Williams K (1998) Electrophoresis 19:845
Chevallet M, Santoni V, Poinas A, Rouquie D, Fuchs A, Kieffer S, Rossignol M, Lunardi J, Garin J, Rabilloud T (1998) Electrophoresis 19:1901
Lopez MF, Berggren K, Chernokalskaya E, Lazarev A, Robinson M, Patton WF (2000) Electrophoresis 21:3673
Chalmers MJ, Gaskell SJ (2000) Curr Opin Biotechnol 11:384
Gygi SP, Aebersold R (2000) Curr Opin Chem Biol 4:489
Molloy M, Herbert B, Walsh B, Tyler M, Traini M, Sanchez J-C, Hochstrasser D, Williams K, Gooley A (1998) Electrophoresis 19:837
Cordwell SJ, Nouwens AS, Verrills NM, Basseal DJ, Walsh BJ (2000) Electrophoresis 21: 1094
Nouwens AS, Cordwell SJ, Larsen MR, Molloy MP, Gillings M, Willcox MDP, Walsh BJ (2000) Electrophoresis 21:3797
Michea-Hamzehpour M, Sanchez J-C, Epp S, Paquet N, Hughes G, Hochstrasser D, Pechere J-C ( 1993) Enzyme Prot 47:1
Cowell BA, Willcox MDP, Herbert B, Schneider RP (1999) J Appl Microbiol 86:944
Cordwell SJ, Wilkins MR, Cerpa-Poljak A, Gooley AA, Duncan M, Williams KL, Humphery-Smith I (1995) Electrophoresis 16:438
Cordwell SJ, Humphery-Smith I (1997) Electrophoresis 18:1410
O’Connor CD, Adams P, Alefounder P, Farris M, Kinsella N, Li Y, Payot S, Skipp P (2000) Electrophoresis 21:1178
Quadroni M, James P, Dainese-Hatt P, Kertesz MA (1999) Eur J Biochem 266:986
Hanna SL, Sherman NE, Kinter MT, Goldberg JB (2000) Microbiology 146:2495
Urquhart-Grindlinger BL (2001) PhD Thesis, University of Sydney
Masuda N, Sakagawa E, Ohya S (1995) Antimicrob Agents Chemother 39:645
Nikaido H, Ocusu H, Ma D, Li X-H (1996) In: Nakazawa T (ed) Molecular biology of pseudomonads. ASM Press, Washington DC, p 353
von Specht B, Knapp B, Hungerer K, Lucking C, Schmitt A, Domdey H (1996) J Biotechnol 44:145
Mansouri E, Gabelsberger J, Knapp B, Hundt E, Lenz U, Hungerer KD, Gilleland HE Jr, Staczek J, Domdey H, von Specht BU (1999) Infect Immun 67:1461
Lee NG, Jung SB, Ahn BY, Kim YH, Kim JJ, Kim DK, Kim IS, Yoon SM, Nam SW, Kim HS, Park WJ (2000) Vaccine 18:1952
Molloy MP, Herbert BR, Slade MB, Rabilloud T, Nouwens AS, Williams KL, Gooley AA (2000) Eur J Biochem 267:2871
Rabilloud T, Blisnick T, Heller M, Luche S, Aebersold R, Lunardi J, Braun-Breton C (1999) Electrophoresis 20:3603
Trias J, Nikaido H (1990) J Biol Chem 265:15,680
Ochs MM, Lu CD, Hancock REW, Abdelal AT (1999) J Bacteriol 181:5426
Nicas TI, Hancock REW (1983) J Bacteriol 153:281
Rawling EG, Brinkman FSL, Hancock REW (1998) J Bacteriol 180:3556
Price BM, Galloway DR, Baker NR, Gilleland LB, Staczek J, Gilleland HE Jr (2001) Infect Immun 69:3510
De Kievit TR, Iglewski BH (2000) Infect Immun 68:4839
Klinger JD, Straus DC, Hilton CB, Bass JA (1978) J. Infect Dis 138:49
Cahan R, Axelrad I, Safrin M, Ohman DE, Kessler E (2001) J Biol Chem 276:43,645
Whiteley M, Lee KM, Greenberg EP (1999) Proc Natl Acad Sci USA 96:13,904
Fuqua C, Greenberg EP (1998) Curr Opin Microbiol 1:183
Pesci EC, Iglewski BH (1997) Trends Microbiol 5:132
Schaffer C, Graninger M, Messner P (2001) Proteomics 1:248
Brimer C, Montie T (1998) J Bacteriol 180:3209
Arora SK, Bangera M, Lory S, Ramphal R (2001) Proc Natl Acad Sci USA 98:9342
Schurr MJ, Yu H, Martinez-Salazar JM, Hibler NS, Deretic V (1995) Biochem Biophys Res Comm 216:874
Jorgensen R, Bally M, Chapon-Herve V, Michel G, Lazdunski A, Williams P, Stewart GSAB (1999) Microbiology 145:835
Yahr TL, Mende-Mueller LM, Friese MB, Frank DW (1997) J Bacteriol 179:7165
Frank DW (1997) Mol Microbiol 26:621
Natera SH, Guerreiro N, Djordjevic MA (2000) Mol Plant Microbe Interact 13:995
Morris AC, Djordjevic MA (2001) Electrophoresis 22:586
Jungblut PR, Muller EC, Mattow J, Kaufmann SH (2001) Infect Immun 69:5905
Goldberg JB, Ohman DE (1987) J Bacteriol 169:1349
Kessler E, Safrin M, Abrams WR, Rosenbloom J, Ohman DE (1997) J Biol Chem 272: 9884
Vessillier S, Delolme F, Bernillon J, Saulnier J, Wallach J (2001) Eur J Biochem 268:1049
Vincent TS, Fraylick JE, McGuffie EM, Olson JC (1999) Mol Microbiol 32:1054
Olson JC, Fraylick JE, McGuffie EM, Dolan KM, Yahr TL, Frank DW, Vincent TS (1999) Infect Immun 67:2847
Hassan HM, Fridovich I (1980) J Bacteriol 141:156
Engels W, Endert J, Kamps MA, van Boven CP (1985) Infect Immun 49:182
Goldberg JB, Pler GB (1996) Trends Microbiol 4:490
Rocchetta HL, Burrows LL, Lam JS (1999) Microbiol Mol Biol Rev 63:523
May TB, Chakrabarty AM (1994) Trends Microbiol 2:151
Gacesa P (1998) Microbiology 144:1133
Stanislavsky ES, Lam JS (1997) FEMS Microbiol Rev 21:243
Feldman M, Bryan R, Rajan S, Scheffler L, Brunnert S, Tang H, Prince A (1998) 66:43
Britigan BE, Rasmussen GT, Cox CD (1997) Infect Immun 65:1071
Songer JG (1997) Trends Microbiol 5:156
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Nouwens, A.S., Walsh, B.J., Cordwell, S.J. (2003). Application of Proteomics to Pseudomonas aeruginosa . In: Hecker, M., et al. Proteomics of Microorganisms. Advances in Biochemical Engineering/Biotechnology, vol 83. Springer, Berlin, Heidelberg. https://doi.org/10.1007/3-540-36459-5_5
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