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Microbial Root Endophytes pp 337–354Cite as

Application of Molecular Fingerprinting Techniques to Explore the Diversity of Bacterial Endophytic Communities

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Part of the book series: Soil Biology ((SOILBIOL,volume 9))

19.7 Conclusions

Molecular fingerprinting methods are required as tools to supplement conventional methods in endophyte research to study cultivable and non-cultivable populations in plants. The intricacies of plants make these techniques difficult to perform. The presence of chloroplast and mitochondrial DNA in total plant DNA extracts, the abundance of plant DNA versus bacterial DNA, and the presence of (poly) phenolics may hamper subsequent analyses. Group (taxon)-specific primer systems can circumvent co-amplification of 16S rDNA sequences from plant cell organelles. The plant metagenome concept offers great perspectives for isolating new substances from plant-associated microorganisms, which may be important for the pharmaceutical and crop protection industries.

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Authors and Affiliations

  1. Plant Research International B.V, Droevendaalsesteeg 1, 6708 PB, Wageningen, The Netherlands

    Leo S. van Overbeek & Jim van Vuurde

  2. Department of Microbial Ecology, Biological Center, Groningen University, Kerklaan 30, 9751 NN, Haren, The Netherlands

    Jan D. van Elsas

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  1. Leo S. van Overbeek
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  2. Jim van Vuurde
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  3. Jan D. van Elsas
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Editors and Affiliations

  1. Institute of Microbiology, Technical University of Braunschweig, Spielmannstraße 7, 38106, Braunschweig, Germany

    Barbara J. E. Schulz

  2. Augustastraße 32, 02826, Görlitz, Germany

    Christine J. C. Boyle

  3. Department of Environmental Sciences Institute of Integrative Biology Forest Pathology and Dendrology, Swiss Federal Institute of Technology, 8092, Zürich, Switzerland

    Thomas N. Sieber

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van Overbeek, L.S., van Vuurde, J., van Elsas, J.D. (2006). Application of Molecular Fingerprinting Techniques to Explore the Diversity of Bacterial Endophytic Communities. In: Schulz, B.J.E., Boyle, C.J.C., Sieber, T.N. (eds) Microbial Root Endophytes. Soil Biology, vol 9. Springer, Berlin, Heidelberg. https://doi.org/10.1007/3-540-33526-9_19

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