Paternal Dual Barrier by Ifg2-H19 and Dlk1-Gtl2 to Parthenogenesis in Mice
The functional difference between the maternal and paternal genome, which is characterized by epigenetic modifications during gametogenesis, that is genomic imprinting, prevents mammalian embryos from parthenogenesis. Genomic imprinting leads to nonequivalent expression of imprinted genes from the maternal and paternal alleles. However, our research showed that alteration of maternal imprinting by oocyte reconstruction using nongrowing oocytes together with deletion of the H19 gene, provides appropriate expression of maternally imprinted genes. Here we discuss that further alteration of paternally imprinted gene expressions at chromosomes 7 and 12 allows the ng/fg parthenogenetic embryos to develop to term, suggesting that the paternal contribution is obligatory for the descendant.
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- Hiura H, Obata Y, Komiyama J, Shirai M, Kono T (2006) Oocyte growth-dependent progression of maternal imprinting in mice. Genes Cells, in pressGoogle Scholar
- Kobayashi S, Wagatsuma H, Ono R, Ichikawa H, Yamazaki M, Tashiro H, Aisaka K, Miyoshi N, Kohda T, Ogura A, Ohki M, Kaneko-Ishino T, Ishino F (2000) Mouse Peg9/Dlk1 and human PEG9/DLK1 are paternally expressed imprinted genes closely located to the maternally expressed imprinted genes: mouse Meg3/Gtl2 and human MEG3. Genes Cells 5:1029–1037PubMedCrossRefGoogle Scholar
- Paulsen M, Takada S, Youngson NA, Benchaib M, Charlier C, Segers K, Georges M, Ferguson-Smith AC (2001) Comparative sequence analysis of the imprinted Dlk1-Gtl2 locus in three mammalian species reveals highly conserved genomic elements and refines comparison with the Igf2-H19 region. Genome Res 11:2085–2094PubMedCrossRefGoogle Scholar
- Schmidt JV, Matteson PG, Jones BK, Guan XJ, Tilghman S (2000) The Dlk1 and Gtl2 genes are linked and reciprocally imprinted. Genes Dev 15:1997–2002Google Scholar
- Surani MA, Kothary R, Allen ND, Singh PB, Fundele R, Ferguson-Smith AC, Barton SC (1990) Genome imprinting and development in the mouse. Dev Suppl:89–98Google Scholar
- Wu Q, Kumagai T, Kawahara M, Ogawa H, Hiura H, Obata Y, Takano R, Kono T (2006) Regulated expressions of two sets of paternally imprinted genes are necessary for mouse parthenogenetic development to term. Reproduction, in pressGoogle Scholar