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Transplantation of Germ Line Stem Cells for the Study and Manipulation of Spermatogenesis

  • Conference paper
Stem Cells in Reproduction and in the Brain

Part of the book series: Ernst Schering Research Foundation Workshop ((SCHERING FOUND,volume 60))

Abstract

Transplantation of male germ line stem cells from a fertile donor to the testis of an infertile recipient restores donor-derived spermatogenesis in the recipient testis and the resulting sperm pass the donor genotype to the offspring of the recipient. Germ cell transplantation has been an invaluable tool to elucidate the biology of male germ line stem cells and their niche in the testis, develop systems to isolate and culture spermatogonial stem cells, examine defects in spermatogenesis, correct male infertility and introduce genetic changes into the male germ line. Although most widely studied in rodents, germ cell transplantation has been applied to larger mammals, including primates. Recently, ectopic grafting of testis tissue from diverse donor species, including primates, into a mouse host has opened an additional possibility to study spermatogenesis and to produce fertile sperm from immature donors. Testis xenografts are ideally suitable to study toxicants or drugs with the potential to enhance or suppress male fertility without the necessity of performing experiments in the target species. Therefore, transplantation of germ cells or xenografting of testis tissue represent powerful approaches for the study, preservation, and manipulation of male fertility.

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Authors and Affiliations

  1. Center for Animal Transgenesis and Germ Cell Research, School of Veterinary Medicine, University of Pennsylvania, Kennett Square, PA, 19348, USA

    I. Dobrinski

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  1. I. Dobrinski
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Editors and Affiliations

  1. Nihon Schering K.K. Research Center, BMA 3F, 1-5-5 Minatojima-minai-machi, Chuo-ku, Kobe, 650-0047, Japan

    J. Morser

  2. Center for Developmental Biology, 2-2-3 Minatojima minami-machi, Chuo-ku, Kobe, 650-0047, Japan

    S. -I. Nishikawa

  3. Max Planck Institute for Molecular Biomedicine, Mendelstr. 7, 48149, Münster, Germany

    H. R. Schöler

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Dobrinski, I. (2006). Transplantation of Germ Line Stem Cells for the Study and Manipulation of Spermatogenesis. In: Morser, J., Nishikawa, S.I., Schöler, H.R. (eds) Stem Cells in Reproduction and in the Brain. Ernst Schering Research Foundation Workshop, vol 60. Springer, Berlin, Heidelberg . https://doi.org/10.1007/3-540-31437-7_12

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