A new simplified technique for producing platelet-rich plasma: a short technical note

  • S. Marlovits
  • M. Mousavi
  • C. Gäbler
  • J. Erdös
  • V. Vécsei
Conference paper


A possible strategy to promote the wound-healing cascade in both soft and hard tissues is the preparation of an autologous plateletrich plasma (PRP) to encourage the release of growth factors from activated platelets. In this process, PRP combines the advantage of an autologous fibrin clot that will aid in hemostasis as well as provide growth factors in high concentrations to the site of a tissue defect. The PRP preparation can be used as a biological enhancer in the healing of fractures and lumbar fusions. The local application of growth factors seems to promote initiation and early maturation of bone formation. Autologous bone or bone substitutes can be added to this mixture to increase the volume of grafting material. A simplified technique utilizing a commercially available separation system (GPS - Gravitational Platelet Separation System) is described. This system provides a less costly alternative to other previously described augmentation techniques and also presents a patient-friendly and operator-safe alternative. Further experimental studies of the actual concentrations of the growth factors in the PRP samples are necessary in order to validate the platelet concentration and growthfactor activation by laboratory evidence. In further prospective clinical trials, the safety and efficacy of PRP, in combination with autologous bone or bone graft substitutes, must be evaluated.


Platelet concentrate Growth factors Bone healing Platelet-rich plasma GPS 


Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.


  1. 1.
    Bhanot S, Alex JC (2002) Current applications of platelet gels in facial plastic surgery. Facial Plast Surg;18:27–33CrossRefPubMedGoogle Scholar
  2. 2.
    Boden SD (2002) Overview of the biology of lumbar spine fusion and principles for selecting a bone graft substitute. Spine 27:S26–31CrossRefPubMedGoogle Scholar
  3. 3.
    Boden SD, Kang J, Sandhu H, Heller JG (2002) Use of recombinant human bone morphogenetic protein-2 to achieve posterolateral lumbar spine fusion in humans: a prospective, randomized clinical pilot trial: 2002 Volvo Award in clinical studies. Spine 27: 2662–2673CrossRefPubMedGoogle Scholar
  4. 4.
    Canalis E (1985) Effect of growth factors on bone cell replication and differentiation. Clin Orthop 193:246–263PubMedGoogle Scholar
  5. 5.
    Canalis E, McCarthy TL, Centrella M (1989) Effects of platelet-derived growth factor on bone formation in vitro. J Cell Physiol 140:530–537CrossRefPubMedGoogle Scholar
  6. 6.
    Caplan AI (1991) Mesenchymal stem cells. J Orthop Res 9:641–650CrossRefPubMedGoogle Scholar
  7. 7.
    Gospodarowicz D (1983) Growth factors and their action in vivo and in vitro. J Pathol 141:201–233CrossRefPubMedGoogle Scholar
  8. 8.
    Hotz G (1991) Alveolar ridge augmentation with hydroxylapatite using fibrin sealant for fixation. I. An experimental study. Int J Oral Maxillofac Surg 20: 204–207CrossRefPubMedGoogle Scholar
  9. 9.
    Hotz G (1991) Alveolar ridge augmentation with hydroxylapatite using fibrin sealant for fixation. II. Clinical application. Int J Oral Maxillofac Surg 20: 208–213CrossRefPubMedGoogle Scholar
  10. 10.
    Howes R, Bowness JM, Grotendorst GR, Martin GR, Reddi AH (1988) Platelet-derived growth factor enhances demineralized bone matrixinduced cartilage and bone formation. Calcif Tissue Int 42:34–38PubMedGoogle Scholar
  11. 11.
    Kasperk CH, Wergedal JE, Mohan S, Long DL, Lau KH, Baylink DJ (1990) Interactions of growth factors present in bone matrix with bone cells: effects on DNA synthesis and alkaline phosphatase. Growth Factors 3:147–158PubMedGoogle Scholar
  12. 12.
    Lowery GL, Kulkarni S, Pennisi AE (1999) Use of autologous growth factors in lumbar spinal fusion. Bone 25: 47S–50SCrossRefPubMedGoogle Scholar
  13. 13.
    Margolis DJ, Kantor J, Santanna J, Strom BL, Berlin JA (2001) Effectiveness of platelet releasate for the treatment of diabetic neuropathic foot ulcers. Diabetes Care 24:483–488PubMedGoogle Scholar
  14. 14.
    Martinowitz U, Spotnitz WD (1997) Fibrin tissue adhesives. Thromb Haemost 78:661–666PubMedGoogle Scholar
  15. 15.
    Marx RE, Carlson ER, Eichstaedt RM, Schimmele SR, Strauss JE, Georgeff KR (1998) Platelet-rich plasma: growth factor enhancement for bone grafts. Oral Surg Oral Med Oral Pathol Oral Radiol Endod 85:638–646CrossRefPubMedGoogle Scholar
  16. 16.
    Matras H (1985) Fibrin seal: the state of the art. J Oral Maxillofac Surg 43: 605–611PubMedGoogle Scholar
  17. 17.
    Nielsen HM, Andreassen TT, Ledet T, Oxlund H (1994) Local injection of TGF-beta increases the strength of tibial fractures in the rat. Acta Orthop Scand 65:37–41PubMedGoogle Scholar
  18. 18.
    Nimni ME (1997) Polypeptide growth factors: targeted delivery systems. Biomaterials 18:1201–1225CrossRefPubMedGoogle Scholar
  19. 19.
    Noda M, Camilliere JJ (1989) In vivo stimulation of bone formation by transforming growth factor-beta. Endocrinology 124:2991–2994PubMedGoogle Scholar
  20. 20.
    Schilephake H (2002) Bone growth factors in maxillofacial skeletal reconstruction. Int J Oral Maxillofac Surg 31:469–484CrossRefPubMedGoogle Scholar
  21. 21.
    Slater M, Patava J, Kingham K, Mason RS (1995) Involvement of platelets in stimulating osteogenic activity. J Orthop Res 13:655–663CrossRefPubMedGoogle Scholar
  22. 22.
    Sonnleitner D, Huemer P, Sullivan DY (2000) A simplified technique for producing platelet-rich plasma and platelet concentrate for intraoral bone grafting techniques: a technical note. Int J Oral Maxillofac Implants;15:879–882PubMedGoogle Scholar
  23. 23.
    Vaccaro AR, Anderson DG, Toth CA (2002) Recombinant human osteogenic protein-1 (bone morphogenetic protein-7) as an osteoinductive agent in spinal fusion. Spine 27:S59–65CrossRefPubMedGoogle Scholar
  24. 24.
    Whitman DH, Berry RL, Green DM (1997) Platelet gel: an autologous alternative to fibrin glue with applications in oral and maxillofacial surgery. J Oral Maxillofac Surg 55:1294–1299CrossRefPubMedGoogle Scholar

Copyright information

© Springer-Verlag Berlin Heidelberg 2005

Authors and Affiliations

  • S. Marlovits
    • 1
    • 2
  • M. Mousavi
    • 1
  • C. Gäbler
    • 1
  • J. Erdös
    • 1
  • V. Vécsei
    • 1
    • 2
  1. 1.Department of TraumatologyMedical University of ViennaViennaAustria
  2. 2.Ludwig Boltzmann Institute for Biomechanics and Cell BiologyViennaAustria

Personalised recommendations