Abstract
A protocol has been developed illustrating the link between validation experiments and measurement uncertainty evaluation. The application of the protocol is illustrated with reference to a method for the determination of three markers (CI solvent red 24, quinizarin and CI solvent yellow 124) in fuel oil samples. The method requires the extraction of the markers from the sample matrix by solid phase extraction followed by quantification by high performance liquid chromatography (HPLC) with diode array detection. The uncertainties for the determination of the markers were evaluated using data from precision and trueness studies using representative sample matrices spiked at a range of concentrations, and from ruggedness studies of the extraction and HPLC stages.
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References
Barwick VJ, Ellison SLR (1999) Accred Qual Assur (in press)
May EM, Hunt DC, Holcombe DG (1986) Analyst 111:993–995
Ellison SLR, Barwick VJ (1998) Accred Qual Assur 3:101–105
Ellison SLR, Barwick VJ (1998) Analyst 123:1387–1392
ISO 9004-4: 1993 (1993) Total quality management Part 2. Guidelines for quality improvement. ISO, Geneva, Switzerland
EURACHEM (1998) The fitness for purpose of analytical methods, a laboratory guide to method validation and related topics. Laboratory of the Government Chemist, London
EURACHEM (1995) Quantifying uncertainty in analytical measurement. Laboratory of the Government Chemist, London
Farrant TJ (1997) Practical statistics for the analytical scientist: a bench guide. Royal Society of Chemistry, Cambridge
ISO 5725: 1994 (1994) Accuracy (trueness and precision) of measurement methods and results. ISO, Geneva, Switzerland
Ellison SLR, Williams A (1996) In: Parkanay M (ed) The use of recovery factors on trace analysis. Royal Society of Chemistry, Cambridge
Youden WJ, Steiner EH (1975) Statistical manual of the association of official analytical chemists. Association of Official Analytical Chemists, Arlington, Va
Brown PR, Hartwick RA (eds) (1989) High performance liquid chromatography. Wiley, New York
Dolan JW (1997) LC-GC International 10: 418–422
Barwick VJ, Ellison SLR (1998) Anal Comm 35: 377–383
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© 2000 Springer-Verlag
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Barwick, V.J., Ellison, S.L., Rafferty, M.J., Gill, R.S. (2000). The evaluation of measurement uncertainty from method validation studies. In: De Bièvre, P., Günzler, H. (eds) Validation in Chemical Measurement. Springer, Berlin, Heidelberg. https://doi.org/10.1007/3-540-27034-5_17
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DOI: https://doi.org/10.1007/3-540-27034-5_17
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Online ISBN: 978-3-540-27034-8
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