Access this chapter
Tax calculation will be finalised at checkout
Purchases are for personal use only
Preview
Unable to display preview. Download preview PDF.
References
Rotman B (1961) Measurement of activity of single molecules of β-D-galactosidase. Proc Natl Acad Sci USA 47:1981–1991
Rotman B (1973) Measurement of single molecules of antibody by their ability to activate a deflective enzyme. In: Thaer AA, Sernetz M (eds) Fluorescence techniques in cell biology. Springer, Berlin Heidelberg New York, pp 333–337
Hirschfeld T (1976) Optical microscopic observation of single small molecules. Appl Opt 15:2965–2966
Hirschfeld T (1976) Quantum efficiency independence of the time integrated emission from a fluorescent molecule. Appl Opt 15:3135–3138
Dovichi NJ, Martin JC, Jett JH, Keller RA (1983) Attogram detection limit for aqueous dye samples by laser-induced fluorescence. Science 219:845–847
Shera EB, Seitzinger NK, Davis LM, Keller RA, Soper SA (1990) Detection of single fluorescent molecules. Chem Phys Lett 174:553–557
Meixner AJ (1998) Optical single-molecule detection at room temperature. Adv Photochem 24:1–59
Xie XS, Trautman JK (1998) Optical studies of single molecules at room temperature. Annu Rev Phys Chem 49:441–480
Enderlein J, Ambrose WP, Goodwin PM, Keller RA (1999) Fluorescence detection of single molecules applicable to small volume assays. In: Köhler M, Mejevaia T, Saluz HP (eds) Microsystem technology: A powerful tool for biomolecular studies. Birkhäuser, Basel, pp 311–329
Weiss S (1999) Fluorescence spectroscopy of single biomolecules. Science 283:1676–1683
Ambrose WP, Goodwin PM, Jett JH, van Orden A, Werner JH, Keller RA (1999) Single molecule fluorescence spectroscopy at ambient temperature. Chem Rev 99:2929–2956
Ishii Y, Yanagida T (2000) Single molecule detection in life science. Single Mol 1:5–14
Keller RA, Emory SR, Ambrose WP, Goodwin PM, Arias AA, Jett JJ, Cai H (2002) Analytical applications of single-molecule detection. Anal Chem 74:316–324A
Rigler R, Orrit M, Basché T (eds) (2001) Single molecule spectroscopy. Springer, Berlin Heidelberg New York
Zander C, Enderlein J, Keller RA (eds) (2002) Single molecule detection in solution. Wiley-VCH, Berlin
Orrit M, Bernard J, Brown R, Lounis B (1996) Optical spectroscopy of single molecule in solids. In: Wolf E (ed) Progress in optics, vol XXXV. Elsevier, Amsterdam, pp 63–144
Trautman JK, Macklin JJ, Brus LE, Betzig E (1994) Near-field spectroscopy of single molecules at room temperature. Nature 369:40–42
Trautman JK, Macklin JJ (1996) Time-resolved spectroscopy of single molecules using near-field and far-field optics. Chem Phys 205:221–229
Trautman JK, Ambrose WP (1997) Near-field optical imaging and spectroscopy of single molecules. In: Basché T, Moerner WE, Orrit M, Wild UP (eds) Single molecule optical detection, imaging and spectroscopy. VCH, Weinheim, pp 191–222
Ruiter AGT, Veerman JA, Garcia-Parajo MF, van Hulst NF (1997) Single-molecule rotational and translational diffusion observed by near-field scanning optical microscopy. J Phys Chem A 101:7318–7323
Garcia-Parajo MF, Veerman JA, van Noort SJT, Grooth BG de, Greve J, van Hulst NF (1998) Near-field optical microscopy for DNA studies at the single molecular level. Bioimaging 6:43–53
Garcia-Parajo MF, Veerman JA, Segers-Nolten GMJ, de Grooth BG, Greve J, van Hulst NF (1999) Visualising individual green fluorescent proteins with a near field optical microscope. Cytometry 36:239–246
Gersen H, Garcia-Parajo MF, Novotny L, Veerman JA, Kuipers L, van Hulst NF (2000) Influencing the angular emission of a single molecule. Phys Rev Lett 85:5312–5315
Gersen H, Garcia-Parajo MF, Novotny L, Veerman JA, Kuipers L, van Hulst NF (2001) Nearfield effects in single molecule emission. J Microsc 202:374–378
van Hulst NF, Veerman JA, García-Parajó MF, Kuipers L (2000) Analysis of individual (macro)molecules and proteins using near-field optics. J Chem Phys 112:7790–7810
Göhbde W, Fischer UC, Fuchs H, Tittel J, Basché T, Bräuchle C, Herrmann A, Müllen K (1998) Fluorescence blinking and photobleaching of single terrylenediimide molecules studied with a confocal microscope. J Phys Chem A 102:9109–9116
Panzer O, Göhde W, Fischer UC, Fuchs H, Müllen K (1998) Influence of oxygen on single molecule blinking. Adv Mat 10:1469–1472
Weston KD, Buratto SK (1998) Millisecond intensity fluctuations of single molecules at room temperature. J Phys Chem A 102:3635–3638
Garcia-Parajo MF, Segers-Nolten GMJ, Veerman JA, Greve J, van Hulst NF (2000) Real-time light-driven dynamics of the fluorescence emission in single green fluorescent protein molecules. Proc Natl Acad Sci USA 97:7237–7242
Dickson RM, Cubitt AB, Tsien RY, Moerner WE (1997) On/off blinking and switching behavior of single molecules of green fluorescent protein. Nature 388:355–358
Moerner WE (2002) Single-molecule optical spectroscopy of autofluorescent proteins. J Chem Phys 117:10925–10937
Chen Y, Müller JD, Ruan QQ, Gratton E (2002) Molecular brightness characterization of EGFP in vivo by fluorescence fluctuation spectroscopy. Biophys J 82:133–144
Kubitscheck U, Kuckmann O, Kues T, Peters R (2000) Imaging and tracking of single GFP molecules in solution. Biophys J 78:2170–2179
Chan WCW, Nie S (1998) Quantum dot bioconjugates for ultrasensitive nonisotopic detection. Science 281:2016–2019
Han M, Gao X, Su JZ, Nie S (2001) Quantum-dot-tagged microbeads for multiplexed optical coding of biomolecules. Nat Biotechnol 19:631–635
Dahan M, Laurence T, Pinaud F, Chemla DS, Alivisatos AP, Sauer M, Weiss S (2001) Time-gated biological imaging by use of colloidal quantum dots. Opt Lett 26:825–827
Dahan M, Levi S, Luccardini C, Rostaing P, Riveau B, Triller A (2003) Diffusion dynamics of glycine receptors revealed by single-quantum dot tracking. Science 302:442–445
Jelezko F, Tietz C, Gruber A, Popa I, Nizovtsev A, Kilin S, Wrachtrup J (2001) Spectroscopy of single N-V centers in diamond. Single Mol 2:255–260
Lee TH, Gonzalez JI, Dickson RM (2002) Strongly enhanced field-dependent single-molecule electroluminescence. Proc Natl Acad Sci USA 99:10272–10275
Peyser LA, Vinson AE, Bartko AP, Dickson RM (2001) Photoactivated fluorescence from individual silver nanoclusters. Science 291:103–106
Boyer D, Tamarat P, Maali A, Orrit M, Lounis B (2003) Imaging single metal nanoparticles in scattering media by photothermal interference contrast. Physica E 17:537–540
Maali A, Cardinalb T, Treguer-Delapierre M (2003) Intrinsic fluorescence from individual silver nanoparticles. Physica E 17:559–560
Cognet L, Tardin C, Boyer D, Choquet D, Tamarat P, Lounis B (2003) Single metallic nanoparticle imaging for protein detection in cells. Proc Natl Acad Sci USA 100:11350–11355
Soper SA, Wabuyele M, Owens CV, Hammer RP (2002) Single molecule detection in the near-infrared. In: Zander C, Enderlein J, Keller RA (eds) Single-molecule detection in solution — methods and applications. Wiley-VCH, Berlin, pp 323–362
Arden-Jacob J, Frantzeskos J, Kemnitzer NU, Zilles A, Drexhage KH (2001) New fluorescent markers for the red region. Spectrochim Acta A 57:2271–2283
Lakowicz JR (2001) Radiative decay engineering: biophysical and biomedical applications. Anal Biochem 298:1–24
Enderlein J (2002) Theoretical study of single molecule fluorescence in a metallic nanocavity. Appl Phys Lett 80:315–317
Enderlein J (2002) Spectral properties of a fluorescing molecule within a spherical metallic nanocavity. Phys Chem Chem Phys 4:2780–2786
Kachel V, Fellner-Feldegg H, Menke E (1990) Hydrodynamic properties of flow cytometry instruments. In: Melamed MR, Lindmo T, Mendelsohn ML (eds) Flow cytometry and sorting. Wiley-Liss, New York, pp 27–44
van Orden A, Machara NP, Goodwin PM, Keller RA (1998) Single molecule identification in flowing sample streams by fluorescence burst size and intraburst fluorescence decay rate. Anal Chem 70:1444–1451
Ambrose WP, Cai H, Goodwin PM, Jett JH, Habbersett RC, Larson EJ, Grace WK, Werner JH, Marrone BL, Keller RA (2000) Flow fragment sizing of DNA fragments. In: Lakowicz JR (ed) Topics in fluorescence, vol 7. Plenum, New York
Larson EJ, Hakovirta JR, Cai H, Jett JH, Burde S, Keller RA, Marrone BL (2000) Rapid DNA fingerprinting of pathogens by flow cytometry. Cytometry 41:203–208
Cai H, White PS, Torney D, Deshpande A, Wang Z, Marrone B, Nolan JP (2000) Flow cytometry-based minisequencing: a new platform for high-throughput single-nucleotide polymorphism scoring. Genomics 66:135–143
Lee YH, Maus RG, Smith BW, Winefordner JD (1994) Laser-induced fluorescence detection of a single molecule in a capillary. Anal Chem 66:4142–4149
Guenard RD, King LA, Smith BW, Winefordner JD (1997) 2-Channel sequential single-molecule measurement. Anal Chem 69:2426–2433
Zander C, Drexhage KH, Han KT, Wolfrum J, Sauer M (1998) Single-molecule counting and identification in a microcapillary. Chem Phys Lett 286:457–465
Neumann M, Ehrfeld W, Han KT, Jacob P, Konrad R, Siebert S, Wolfrum J, Sauer M (1999) Electrophoretic sizing of DNA fragments and detection of single mononucleotide molecules in microstructures. Biomed Chromatogr 13:109–110
Sauer M, Angerer B, Han K-T, Zander C (1999) Detection and identification of single dyelabeled mononucleotide molecules released from an optical fiber in a microcapillary: first steps towards a new single molecule DNA sequencing technique. Phys Chem Chem Phys 1:2471–2477
Becker W, Hickl H, Zander C, Drexhage KH, Sauer M, Siebert S, Wolfrum J (1999) Time-resolved detection and identification of single analyte molecules in microcapillaries by time-correlated single-photon counting (TCSPC). Rev Sci Instrum 70:1835–1841
Fister JC, Jacobson SC, Davis LM, Ramsey JM (1998) Counting single chromophore molecules for ultrasensitive analysis and separations on microchip devices. Anal Chem 70:431–437
Effenhauser CS, Bruin GJM, Paulus A, Ehrat M (1997) Integrated capillary electrophoresis on flexible silicone microdevices: analysis of DNA restriction fragments and detection of single DNA molecules on microchips. Anal Chem 69:3451–3457
Chou HP, Spence C, Scherer A, Quake S (1999) A microfabricated device for sizing and sorting DNA molecules. Proc Natl Acad Sci USA 96:11–13
Foquet M, Korlach J, Zipfel W, Webb WW, Craighead HG (2002) DNA fragment sizing by single molecule detection in submicrometer-sized closed fluidic channels. Anal Chem 74:1415–1422
Chen DY, Adelhelm K, Cheng XL, Dovichi NJ (1994) Simple laser-induced fluorescence detector for sulforhodamine 101 in a capillary electrophoresis system — detection limits of 10 yoctomoles or six molecules. Analyst 119:349–352
Castro A, Shera EB (1995) Single-molecule electrophoresis. Anal Chem 67:3181–3186
Castro A, Shera EB (1995) Single-molecule detection: applications to ultrasensitive biochemical analysis. Appl Opt 34:3218–3222
Castro A, Williams JGK (1997) Single-molecule detection of specific nucleic-acid sequences in unamplified genomic DNA. Anal Chem 69:3915–3920
Haab BB, Mathies RA (1995) Single molecule fluorescence burst detection of DNA fragments separated by capillary electrophoresis. Anal Chem 67:3253–3260
Haab BB, Mathies RA (1997) Optimization of single-molecule fluorescence burst detection of ds-DNA — application to capillary electrophoresis separations of 100-1,000 basepair fragments. Appl Spetrosc 51:1579–1584
Shortreed MR, Li H, Huang WH, Yeung ES (2000) High-throughput single-molecule DNA screening based on electrophoresis. Anal Chem 72:2879–2885
Castro A (2002) Single molecule detection of specific nucleic acid sequences. In: Zander C, Enderlein J, Keller RA (eds) Single-molecule detection in solution — methods and applications. Wiley-VCH, Berlin, pp 303–321
Whitten WB, Arnold JM, Ramsey S, Bronk BV (1991) Single-molecule detection limits in levitated microdroplets. Anal Chem 63:1027–1031
Barnes MD, Ng KC, Whitten WB, Ramsey JM (1993) Detection of single rhodamine 6G molecules in levitated microdroplets. Anal Chem 65:2360–2365
Barnes MD, Lermer N, Kung CY, Whitten WB, Ramsey JM, Hill SC (1997) Real-time observation of single-molecule fluorescence in microdroplet streams. Opt Lett 22: 1265–1267
Lermer N, Barnes MD, Kung CY, Whitten WB, Ramsey JM (1997) High-efficiency molecular counting in solution: single-molecule detection in electrodynamically focused microdroplet streams. Anal Chem 69:2115–2121
Kung CY, Barnes MD, Lermer N, Whitten WB, Ramsey JM (1998) Confinement and manipulation of individual molecules in attoliter volumes. Anal Chem 70:658–661
Barnes MD, Kung C-Y, Whitten WB, Ramsey JM, Arnold S, Holler S (1996) Fluorescence of oriented molecules in a microcavity. Phys Rev Lett 76:3931–3934
Kung CY, Barnes MD, Lermer N, Whitten WB, Ramsey JM (1999) Single-molecule analysis of ultradilute solutions with guided streams of 1-µm water droplets. Appl Opt 38:1481–1487
Hansen RL, Harris JM (1998) Measuring reversible adsorption kinetics of small molecules at solid/liquid interfaces by total internal reflection fluorescence correlation spectroscopy. Anal Chem 70:4247–4256
Hansen RL, Harris JM (1998) Total internal reflection fluorescence correlation spectroscopy for counting molecules at liquid/solid interfaces. Anal Chem 70:2565–2575
Paige MF, Bierneld EJ, Moerner WE (2001) A comparison of through-the-objective total internal reflection microscopy and epifluorescence microscopy for single-molecule fluorescence imaging. Single Mol 2:191–201
Ambrose WP, Goodwin PM, Nolan JP (1999) Single-molecule detection with total internal reflection excitation: comparing signal-to-background and total signals in different geometries. Cytometry 36:224–231
Ruckstuhl T, Enderlein J, Jung S, Seeger S (2000) Forbidden light detection from single molecules. Anal Chem 72:2117–2123
Ruckstuhl T, Rankl M, Seeger S (2003) Highly sensitive biosensing using a supercritical angle fluorescence (SAF) instrument. Biosens Bioelectron 18:1193–1199
Krieg A, Laib S, Ruckstuhl T, Seeger S (2003) Real-time detection of nucleotide incorporation during complementary DNA strand synthesis. Chembiochem 4:589–592
Ruckstuhl T, Seeger S (2003) Confocal total-internal-reflection fluorescence microscopy with a high-aperture parabolic mirror lens. Appl Opt 42:3277–3283
Böhmer M, Pampaloni F, Wahl M, Rahn HJ, Erdmann R, Enderlein J (2001) Advanced time-resolved confocal scanning device for ultrasensitive fluorescence detection. Rev Sci Instrum 72:4145–4152
Böhmer M, Enderlein J (2002) Single molecule detection on surfaces with the confocal laser scanning microscope. In: Zander C, Enderlein J, Keller RA (eds) Single-molecule detection in solution — methods and applications. Wiley-VCH, Berlin, pp 145–183
Funatsu T, Harada Y, Tokunaga M, Saito K, Yanagida T (1995) Imaging of single fluorescent molecules and individual ATP turnovers by single myosin molecules in aqueous solution. Nature 374:555–559
Adachi K, Kinosita K, Ando T (1999) Single-fluorophore imaging with an unmodified epifluorescence microscope and conventional video camera. J Microsc 195:125–132
Straub M, Hell SW (1998) Fluorescence lifetime three-dimensional microscopy with picosecond precision using a multifocal multiphoton microscope. Appl Phys Lett 73:1769–1771
Tokunaga M, Kitamura K, Saito K, Iwane AH, Yanagida T (1997) Single-molecule imaging of fluorophores and enzymatic-reactions achieved by objective-type total internal-reflection fluorescence microscopy. Biochem Biophys Res Commun 235:47–53
Vacha M, Kotani M (2003) Three-dimensional orientation of single molecules observed by far-and near-field fluorescence microscopy. J Chem Phys 118:5279–5282
Mörtelmaier M, Kögler EJ, Hesse J, Sonnleitner M, Huber LA, Schütz GJ (2002) Single molecule microscopy in living cells: subtraction of autofluorescence based on two-color recording. Single Mol 3:225–231
Cognet L, Harms GS, Blab GA, Lommerse PHM, Schmidt T (2000) Simultaneous dualcolor and dual-polarization imaging of single molecules. Appl Phys Lett 77:4052–4054
Ma Y, Shortreed MR, Yeung ES (2000) High-throughput single-molecule spectroscopy in free solution. Anal Chem 72:4640–4645
Schütz GJ, Trabesinger W, Schmidt T (1998) Direct observation of ligand colocalization on individual receptor molecules. Biophys J 74:2223–2226
Schütz GJ, Hesse J, Freudenthaler G, Pastushenkoo VP, Knaus HG, Pragl B, Schindler H (2000) 3D mapping of individual ion channels on living cells. Single Mol 1:153–157
Schütz GJ, Axmann M, Schindler H (2001) Imaging single molecules in three dimensions. Single Mol 2:69–74
Schütz GJ, Schindler H, Schmidt T (1997) Single-molecule microscopy on model membranes reveals anomalous diffusion. Biophys J 73:1073–1080
Schütz GJ, Kada G, Pastushenko VP, Schindler HG (2000) Properties of lipid microdomains in a muscle cell membrane visualized by single molecule microscopy. EMBO J 19:892–901
Schütz GJ, Schindler H (2002) Single dye tracing for ultrasensitive microscopy on living cells. In: Zander C, Enderlein J, Keller RA (eds) Single-molecule detection in solution — methods and applications. Wiley-VCH, Berlin, pp 231–245
Yanagida T, Nakase M, Nishiyama K, Oosawa F (1984) Direct observation of motion of single F-actin filaments in the presence of myosin. Nature 307:58–60
Oiwa K, Eccleston JF, Anson M, Kikumoto M, Davis CT, Reid GP, Ferenczi MA, Corrie JET, Yamada A, Nakayama H, Trentham DR (2000) Comparative single-molecule and ensemble myosin enzymology: sulfoindocyanine ATP and ADP derivatives. Biophys J 78:3048–3071
Okada Y, Hirokawa N (1999) A processive single-headed motor: kinesin superfamily protein KIF1A. Science 283:1152–1157
Inoue Y, Iwane AH, Miyai T, Muto E, Yanagida T (2001) Motility of single one-headed kinesin molecules along microtubules. Biophys J 81:2838–28350
Ishii Y, Iwane AH, Yokota H, Inoue Y, Wazawa T, Nishiyama M, Tanaka H, Kitamura K, Yanagida T (2002) Studying molecular motors on the single molecule level. In: Zander C, Enderlein J, Keller RA (eds) Single-molecule detection in solution — methods and applications. Wiley-VCH, Berlin, pp 273–292
Kubitschek U (2002) Single protein molecules visualized and tracked in the interior of eukaryotic cells. Single Mol 3:267–274
Dickson RM, Norris DJ, Moerner WE (1998) Simultaneous imaging of individual molecules aligned both parallel and perpendicular to the optic axis. Phys Rev Lett 81:5322–5325
Bartko AP, Dickson RM (1999) Imaging three-dimensional singe molecule orientations. J Phys Chem B 103:11237–11241
Bartko AP, Xu K, Dickson RM (2002) Three-dimensional single molecule rotational diffusion in glassy state polymer films. Phys Rev Lett 89:026101
Böhmer M, Enderlein J (2003) Orientation imaging of single molecules by wide-field epifluorescence microscopy. J Opt Soc B 20:554–559
O’Connor DV, Phillips D (1984) Time-correlated single photon counting. Academic, London
Soper SA, Davis LM, Shera EB (1992) Detection and identification of single molecules in solution. J Opt Soc Am B 9:1761–1769
Wilkerson CW, Goodwin PM, Ambrose WP, Martin JC, Keller RA (1993) Detection and lifetime measurement of single molecules in flowing sample streams by laser-induced fluorescence. Appl Phys Lett 62:2030–2032
Li L-Q, Davis LM (1993) Single photon avalanche diode for single molecule detection. Rev Sci Instrum 64:1524–1529
Böhmer M, Wahl M, Rahn HJ, Erdmann R, Enderlein J (2002) Time-resolved fluorescence correlation spectroscopy. Chem Phys Lett 353:439–445
Köllner M, Fischer P, Arden-Jacob J, Drexhage K-H, Müller R, Seeger S, Wolfrum J (1996) Fluorescence pattern recognition for ultrasensitive molecule identification: comparison of experimental data and theoretical predictions. Chem Phys Lett 250:355–360
Enderlein J, Goodwin PM, van Orden A, Ambrose WP, Erdmann R, Keller RA (1997) A maximum likelihood estimator to distinguish single molecules by their fluorescence decays. Chem Phys Lett 270:464–470
Enderlein J, Sauer M (2001) Optimal algorithm for single molecule identification with time-correlated single photon counting. J Phys Chem A 105:48–53
Herten DP, Tinnefeld P, Sauer M (2000) Identification of single fluorescently labeled mononucleotide molecules in solution by spectrally resolved time-correlated single photon counting. Appl Phys B 71:765–771
Magde D, Elson E, Webb WW (1972) Thermodynamic fluctuations in a reacting system — measurement by fluorescence correlation spectroscopy. Phys Rev Lett 29:705–708
Elson EL, Magde D (1974) Fluorescence correlation spectroscopy. I. Conceptual basis and theory. Biopolymers 13:1–27
Magde D, Elson E, Webb WW (1974) Fluorescence correlation spectroscopy. II. An experimental realization. Biopolymers 13:29–61
Widengren J, Mets Ü (2002) Conceptual basis of fluorescence correlation spectroscopy and related techniques as tools in bioscience. In: Zander C, Enderlein J, Keller RA (eds) Single-molecule detection in solution — methods and applications. Wiley-VCH, Berlin, pp 69–95
Petersen NO (1986) Scanning fluorescence correlation spectroscopy. I. Theory and simulation of aggregation measurements. Biophys J 49:809–815
Petersen NO, Johnson DC, Schlesinger MJ (1986) Scanning fluorescence correlation spectroscopy. II. Application to virus glycoprotein aggregation. Biophys J 49:817–820
Amediek A, Haustein E, Scherfeld D, Schwille P (2002) Scanning dual-color cross-correlation analysis for dynamic co-localization studies of immobile molecules. Single Mol 3:201–210
Schwille P, Meyer-Almes FJ, Rigler R (1997) Dual-color fluorescence cross-correlation spectroscopy for multicomponent diffusional analysis in solution. Biophys J 72:1878–1886
Bacia K, Majoul IV, Schwille P (2002) Probing the endocytic pathway in live cells using dual-color fluorescence cross-correlation analysis. Biophys J 83:1184–1193
Kettling U, Koltermann A, Schwille P, Eigen M (1998) Real-time enzyme kinetics monitored by dual-color fluorescence cross-correlation spectroscopy. Proc Natl Acad Sci USA 95:1416–1420
Koltermann A, Kettling U, Bieschke J, Winkler T, Eigen M (1998) Rapid assay processing by integration of dual-color fluorescence cross-correlation spectroscopy: high-throughput screening for enzyme activity. Proc Natl Acad Sci USA 95:1421–1426
Heinze K, Koltermann A, Schwille P (2000) Simultaneous two-photon excitation of distinct labels for dual-color fluorescence cross-correlation analysis. Proc Natl Acad Sci USA 97:10377–10382
Rarbach M, Kettling U, Koltermann A, Eigen M (2001) Dual-color fluorescence cross-correlation spectroscopy for monitoring the kinetics of enzyme-catalyzed reactions. Methods 24:104–116
Heinze KG, Rarbach M, Jahnz M, Schwille P (2002) Two-photon fluorescence coincidence analysis: rapid measurements of enzyme kinetics. Biophys J 83:1671–1681
Balakrishnan J (2000) Spatial curvature effects on molecular transport by diffusion. Phys Rev E 61:4648–46451
Chirico G, Fumagalli C, Baldini G (2002) Trapped Brownian motion in single-and two-photon excitation fluorescence correlation experiments. J Phys Chem B 106:2508–2519
Osborne MA, Balasubramanian S, Furey WS, Klenerman D (1998) Optically biased diffusion of single molecules studied by confocal fluorescence microscopy. J Phys B 102:3160–3167
Egner A, Schrader M, Hell SW (1998) Refractive index mismatch induced intensity and phase variations in fluorescence confocal, multiphoton and 4Pi-microscopy. Opt Commun 153:211–217
Ganic D, Gan X, Gu M (2000) Reduced effects of spherical aberration on penetration depth under two-photon excitation. Appl Opt 39:3945–3947
Fradin C, Abu-Arish A, Granek R, Elbaum M (2003) Fluorescence correlation spectroscopy close to a fluctuating membrane. Biophys J 84:2005–2020
Gennerich A, Schild D (2000) Fluorescence correlation spectroscopy in small cytosolic compartments depends critically on the diffusion model used. Biophys J 79:3294–3306
Korlach J, Schwille P, Webb WW, Feigenson GW (1999) Characterization of lipid bilayer phases by confocal microscopy and fluorescence correlation spectroscopy. Proc Natl Acad Sci USA 69:8461–8466
Gell C, Brockwell DJ, Beddard GS, Radford SE, Kalverda AP, Smith DA (2001) Accurate use of single molecule fluorescence correlation spectroscopy to determine molecular diffusion times. Single Mol 2:177–181
Yoshida N, Tamura M, Kinjo M (2000) Fluorescence correlation spectroscopy: a new tool for probing the microenvironment of the internal space of organelles. Single Mol 1:279–283
Widengren J, Rigler R (1998) Fluorescence correlation spectroscopy as a tool to investigate chemical reactions in solution and on cell surfaces. Cell Mol Biol 44:857–879
Björling S, Kinjo M, Földes-Papp Z, Hagman E, Thyberg P, Rigler R (1998) Fluorescence correlation spectroscopy of enzymatic DNA polymerization. Biochemistry 37:12971–12978
Häsler K, Pänke O, Junge W (1999) On the stator of rotary ATP synthase: the binding strength of subunit d to ab3 as determined by fluorescence correlation spectroscopy. Biochemistry 38:13759–13765
Schwille P, Bieschke J, Oehlenschlager F (1997) Kinetic investigations by fluorescence correlation spectroscopy: the analytical and diagnostic potential of diffusion studies. Biophys Chem 66:211–228
Wohland T, Friedrich K, Hovius R, Vogel H (1999) Study of ligand-receptor interactions by fluorescence correlation spectroscopy with different fluorophores: evidence that the homopentameric 5-hydroxytryptamine Type 3 as receptor binds only one ligand. Biochemistry 38:8671–8681
Widengren J, Mets Ü, Rigler R (1999) Photodynamic properties of green fluorescent proteins investigated by fluorescence correlation spectroscopy. Chem Phys 250:171–186
Haupts U, Maiti S, Schwille P, Webb WW (1998) Dynamics of fluorescence fluctuations in green fluorescent protein observed by fluorescence correlation spectroscopy. Proc Natl Acad Sci USA 95:13573–13578
Heikal AA, Hess ST, Baird GS, Tsien RY, Webb WW (2000) Molecular spectroscopy and dynamics of intrinsically fluorescent proteins: Coral red (dsRed) and yellow (Citrine). Proc Natl Acad Sci USA 97:11996–12001
Brock R, Vàmosi G, Vereb G, Jovin TM (1999) Rapid characterization of green fluorescent protein fusion proteins on the molecular and cellular level by fluorescence correlation microscopy. Proc Natl Acad Sci USA 96:10123–10128
Heikal AA, Hess ST, Webb WW (2001) Multiphoton molecular spectroscopy and excited-state dynamics of enhanced green fluorescent protein (EGFP): acid-base specificity. Chem Phys 274:37–55
Schwille P, Kummer S, Heikal AA, Moerner WE, Webb WW (2000) Fluorescence correlation spectroscopy reveals fast optical excitation-driven intramolecular dynamics of yellow fluorescent proteins. Proc Natl Acad Sci USA 97:151–156
Widengren J, Seidel CAM (2000) Manipulation and characterization of photo-induced transient states of Merocyanine 540 by fluorescence correlation spectroscopy. Phys Chem Chem Phys 2:3435–3441
Huang S, Heikal AA, Webb WW (2002) Two-photon fluorescence spectroscopy and microscopy of NAD(P)H and flavoprotein. Biophys J 82:2811–2825
Malvezzi-Campeggi F, Jahnz M, Heinze KG, Dittrich P, Schwille P (2001) Light-induced flickering of dsRed provides evidence for distinct and interconvertible fluorescent states. Biophys J 81:1776–1785
Widengren J, Schwille P (2000) Characterization of photoinduced isomerization and back-isomerization of the cyanine dye Cy5 by fluorescence correlation spectroscopy. J Phys Chem A 104:6416–6428
Schwille P (2001) Fluorescence correlation spectroscopy and its potential for intracellular applications. Cell Biochem Biophys 34:383–408
Hess ST, Huang S, Heikal AA, Webb WW (2002) Biological and chemical applications of fluorescence correlation spectroscopy: a review. Biochemistry 41:697–705
Rigler R, Elson E (eds) (2001) Fluorescence correlation spectroscopy. Springer, Berlin Heidelberg New York
Kask P, Palo K, Ullmann D, Gall K (1999) Fluorescence-intensity distribution analysis and its application in biomolecular detection technology. Proc Natl Acad Sci USA 96:13756–13761
Kask P, Palo K, Fay N, Brand L, Mets U, Ullmann D, Jungmann J, Pschorr J, Gall K (2000) Two-dimensional fluorescence intensity distribution analysis: theory and applications. Biophys J 78:1703–1713
Chen Y, Müller JD, So PTC, Gratton E (1999) The photon counting histogram in fluorescence fluctuation spectroscopy. Biophys J 77:553–567
Chen Y, Müller JD, Berland KM, Gratton E (1999) Fluorescence fluctuation spectroscopy. In: Conn PM (ed) Methods: a companion to methods in enzymology, vol 19. Academic, London, pp 234–252
Chen Y, Müller JD, Tetin SY, Tyner JD, Gratton E (2000) Probing ligand protein binding equilibria with fluorescence fluctuation spectroscopy. Biophys J 79:1074–1084
Palo K, Mets U, Jager S, Kask P, Gall K (2000) Fluorescence intensity multiple distributions analysis: concurrent determination of diffusion times and molecular brightness. Biophys J 79:2858–2866
Enderlein J, Robbins DL, Ambrose WP, Keller RA (1998) Molecular shot noise, burst size distributions, and single molecule detection in fluid flow: effects of multiple occupancy. J Phys Chem A 102:6089–6094
Fries JR, Brand L, Eggeling C, Köllner M, Seidel CAM (1998) Quantitative identification of different single molecules by selective time-resolved confocal fluorescence spectroscopy. J Phys Chem A 102:6601–6613
Maus M, Cotlet M, Hofkens J, Gensch T, De Schryver FC, Schaffer J, Seidel CAM (2001) An experimental comparison of the maximum likelihood estimation and nonlinear least-squares fluorescence lifetime analysis of single molecules. Anal Chem 73:2078–2086
Knemeyer J-P, Herten D-P, Sauer M (2003) Detection and identification of single molecules in living cells using spectrally resolved fluorescence lifetime imaging microscopy. Anal Chem 75:2147–53
Dahan M, Deniz AA, Ha T, Chemla DS (1999) Ratiometric measurement and identification of single diffusing molecules. Chem Phys 247:85–106
Eggeling C, Fries JR, Brand L, Günther R, Seidel CAM (1998) Monitoring conformational dynamics of a single molecule by selective fluorescence spectroscopy. Proc Natl Acad Sci USA 95:1556–1561
Eggeling C, Schaffer J, Seidel CAM, Korte J, Brehm G, Schneider S, Schrof W (2001) Homogeneity, transport, and signal properties of single Ag particles studied by single-molecule surface-enhanced resonance Raman scattering. J Phys Chem A 105:3673–3679
Schaffer J, Volkmer A, Eggeling C, Subramaniam V, Striker G, Seidel CAM (1999) Identification of single molecules in aqueous solution by time-resolved fluorescence anisotropy. Phys Chem A 103:331–336
Kühnemuth R, Seidel CAM (2001) Principles of single molecule multiparameter fluorescence spectroscopy. Single Mol 2:251–254
Seidel C, personal communication
Börsch M, Turina P, Eggeling C, Fries JR, Seidel CAM, Labahn A, Gräber P (1998) Conformational changes of the H+-ATPase from Escherichia coli upon nucleotide binding detected by single molecule fluorescence. FEBS Lett 437:251–254
Author information
Authors and Affiliations
Editor information
Editors and Affiliations
Rights and permissions
Copyright information
© 2005 Springer-Verlag Berlin Heidelberg
About this chapter
Cite this chapter
Enderlein, J. (2005). Single Molecule Spectroscopy: Basics and Applications. In: Hof, M., Hutterer, R., Fidler, V. (eds) Fluorescence Spectroscopy in Biology. Springer Series on Fluorescence, vol 3. Springer, Berlin, Heidelberg. https://doi.org/10.1007/3-540-27004-3_7
Download citation
DOI: https://doi.org/10.1007/3-540-27004-3_7
Publisher Name: Springer, Berlin, Heidelberg
Print ISBN: 978-3-540-22338-2
Online ISBN: 978-3-540-27004-1
eBook Packages: Chemistry and Materials ScienceChemistry and Material Science (R0)