Skip to main content

Microspore Embryo Induction and Development in Higher Plants: Cytological and Ultrastructural Aspects

  • Chapter
Haploids in Crop Improvement II

Part of the book series: Biotechnology in Agriculture and Forestry ((AGRICULTURE,volume 56))

This is a preview of subscription content, log in via an institution to check access.

Access this chapter

Chapter
USD 29.95
Price excludes VAT (USA)
  • Available as PDF
  • Read on any device
  • Instant download
  • Own it forever
eBook
USD 169.00
Price excludes VAT (USA)
  • Available as PDF
  • Read on any device
  • Instant download
  • Own it forever
Softcover Book
USD 219.99
Price excludes VAT (USA)
  • Compact, lightweight edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info
Hardcover Book
USD 219.99
Price excludes VAT (USA)
  • Durable hardcover edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info

Tax calculation will be finalised at checkout

Purchases are for personal use only

Institutional subscriptions

Preview

Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.

References

  • Alché JD, Castro AJ, Solymoss M, Timar I, Barnabas B, Rodríguez-García MI (2000) Cellular approach to the study of androgenesis in maize anthers: immunocytochemical evidence of the involvement of the ubiquitin degradative pathway in androgenesis induction. J Plant Physiol 156:146–155

    Google Scholar 

  • Binarova P, Hause G, Cenklova V, Cordewener JHG, van Lookeren Campagne MM (1997) A short severe heat shock is required to induce embryogenesis in late bicellular pollen of Brassica napus L. Sexual Plant Reprod 10:200–208

    Article  Google Scholar 

  • Cai Q, Szarejko I, Polok K, Maluszynski M (1992) The effect of sugars and growth regulators on embryoid formation and plant regeneration from barley anther culture. Plant Breed 109:218–226

    CAS  Google Scholar 

  • Caredda S, Clément C (1999) Androgenesis and albinism in Poaceae: influence of genotype and carbohydrates. In: Clément C, Pacini E, Audran JC (eds) Anther and pollen: from biology to biotechnology. Springer, Berlin Heidelberg New York, pp 211–226

    Google Scholar 

  • Caredda S, Devaux P, Sangwan RS, Clément C (1999) Differential development of plastids during microspore embryogenesis in barley. Protoplasma 208:248–256

    Article  Google Scholar 

  • Caredda S, Doncoeur C, Devaux P, Sangwan RS, Clément C (2000) Plastid differentiation during androgenesis in albino and non albino-producing cultivars of barley (Hordeum vulgare L.). Sexual Plant Reprod 13:95–104

    Article  CAS  Google Scholar 

  • Caredda S, Devaux P, Sangwan RS, Proult I, Clément C (2004) Plastid ultrastructure and DNA related to albinism in androgenetic embryos of various barley (Hordeum vulgare L.) cultivars. Plant Cell Tissue Organ Cult 76(1):35–43

    Article  CAS  Google Scholar 

  • Chu CC, Hill RD, Brule-Babel A (1990) High frequency of pollen embryoid formation and plant regeneration in Triticum aestivum L. on monosaccharide containing media. Plant Sci 66:255–262

    Article  CAS  Google Scholar 

  • Cistué L, Ramos A, Castillo AM, Romagosa I (1994) Production of large number of doubled haploid plants from barley anthers pretreated with high concentrations of mannitol. Plant Cell Rep 13:709–712

    Google Scholar 

  • Cordewener JHG, Custers JBM, Dons HJM, van Lookeren Campagne M (1996) Molecular and biochemical events during the induction of microspore embryogenesis. In: Jain MS, Sopory SK, Veilleux RE (eds) In vitro production of higher plants, vol 1. Kluwer, Dordrecht, pp 111–124

    Google Scholar 

  • Custers JBM, Cordewener JHG, Nöllen Y, Dons JJM, van Lookeren Campagne M (1994) Temperature controls both gametophytic and sporophytic development in microspore cultures of Brassica napus. Plant Cell Rep 13:267–271

    Article  CAS  Google Scholar 

  • Devaux P (1992) Haploidy in barley and wheat improvement. In: Dattée A (ed) Reproductive biology and plant breeding. Springer, Berlin Heidelberg New York, pp 139–151

    Google Scholar 

  • Devaux P, Zivy M, Kilian A, Kleinhofs A (1996) Doubled haploids in barley. In: Scoles G, Rossnagel B (eds) Proc 5th Int Oat Conf and 7th Int Barley Genet Symp. University Extenson Press, Saskatoon, Saskatchewan, Canada, pp 213–222

    Google Scholar 

  • Eady C, Lindsey K, Twell D (1995) The significance of microspore divisions and division symmetry for vegetative cell specific transcription and generative cell differentiation. Plant Cell 7:65–74

    Article  PubMed  CAS  Google Scholar 

  • Garrido D, Vicente O, Heberle-Bors E, Rodriguez-Garcia I (1995) Cellular changes during the acquisition of embryogenic potential in isolated pollen grains of Nicotiana tabacum. Protoplasma 186:220–230

    Article  Google Scholar 

  • Goralski G, Matthys-Rochon E, Vergne P, Przywara L (1999) Androgenic development: a fascinating embryo formation process. Acta Biol Cracov 41:51–65

    Google Scholar 

  • Guha S, Maheshwari SC (1964) In vitro production of embryos anther of Datura. Nature 204:497–498

    Google Scholar 

  • Guha S, Maheshwari SC (1966) Cell division and differentiation of embryos in the pollen of Datura in vitro. Nature 212:97–98

    Google Scholar 

  • Hoekstra S, van Zijdervzld MH, Louwerse JD, Heidelkamp F, van der Mark F (1992) Anther and microspore culture of Hordeum vulgare L. Plant Sci 8:89–96

    Google Scholar 

  • Honys D, Twell D (2003) Comparative analysis of the Arabidopsis pollen transcriptome. Plant Physiol 132:640–652

    Article  PubMed  CAS  Google Scholar 

  • Hu T, Kasha KJ (1999) A cytological study of pretreatments used to improve isolated microspores cultures of wheat (Triticum aestivum L.) cv. Chris. Genome 42:432–441

    Article  Google Scholar 

  • Ilic-Grubor K, Attree M, Fowke LC (1998) Comparative morphological study of zygotic and microspore-derived embryos of Brassica napus L. as revealed by scanning electron microscopy. Ann Bot 82:157–165

    Google Scholar 

  • Indrianto A, Barinova I, Touraev A, Heberle-Bors E (2001) Tracking individual wheat microspores in vitro: identification of embryogenic microspores and body axis formation in the embryo. Planta 212:163–174

    Article  PubMed  CAS  Google Scholar 

  • Jacquard C, Wojnarowiez G, Clément C (2003) Anther culture in barley. In: Kasha K, Maluszinski M (eds) Doubled haploid manual. Springer, Berlin Heidelberg New York, pp 21–28

    Google Scholar 

  • Jähne A, Lörz H(1995) Cereal microspore culture. Plant Sci 109:1–12

    Google Scholar 

  • Jardinaud MF, Souvré A, Alibert G, Beckert M (1995a) uidA gene transfer and expression in maize microspores using the biolistic method. Protoplasma 187:138–143

    Article  CAS  Google Scholar 

  • Jardinaud MF, Souvré A, Alibert G, Beckert M (1995b) Optimisation of DNA transfer and transient ß-glucuronidase expression in electroporated maize (Zea mays L.) microspores. Plant Cell Rep 15:55–58

    Article  Google Scholar 

  • Kao KN (1993) Viability, cell division and microcallus formation of barley microspores in culture. Plant Cell Rep 12:366–369

    Article  CAS  Google Scholar 

  • Kasha KJ, Yao Q, Simion E, Hu T, Oro R (1995) Production and applications of haploids in crops. Use of induced mutation and molecular techniques of crop improvement. International Atomic Energy Agency, Vienna, Austria, pp 23–37

    Google Scholar 

  • Knox RB (1971) Pollen wall proteins: localization, enzymic and antigenic activity during development in Gladiolus. J Cell Sci 9:209–237

    PubMed  CAS  Google Scholar 

  • Kumlehn J, Lörz H (1999) Monitoring sporophytic development of individual microspores of barley (Hordeum vulgare L.). In: Clément C, Pacini E, Audran JC (eds) Anther and pollen: from biology to biotechnology. Springer, Berlin Heidelberg New York, pp 183–190

    Google Scholar 

  • Lee JY, Lee DH (2003) Use of serial analysis of gene expression technology to reveal changes in gene expression in Arabidopsis pollen undergoing cold stress. Plant Physiol 132:517–529

    PubMed  CAS  Google Scholar 

  • Loeb TA, Reynolds TL (1994) Transient expression of the uidA gene in pollen embryoids of wheat following microprojectile bombardment. Plant Sci 104:81–91

    Article  CAS  Google Scholar 

  • Luckett DJ, Darvey NL (1992) Utilisation of microspore culture in wheat and barley improvement. Aust J Bot 40:807–828

    Article  Google Scholar 

  • Luckett DJ, Smithard RA (1992) Doubled haploid production by anther culture for Australian barley breeding. Aust J Agric Res 43:67–78

    Google Scholar 

  • Lyne RL, Bennett RI, Hunter CP (1986) Embryoid and plant production from cultured barley anthers. In: Withers LA, Alderson PG (eds) Plant tissue culture and its agricultural application. Butterworths, London, pp 405–410

    Google Scholar 

  • Mackenzie A, Heslop-Harrison J, Dickinson HG (1967) Elimination of ribosomes during meiotic prophase. Nature 215:997–999

    PubMed  CAS  Google Scholar 

  • Magnard JL, Le Deunff E, Domenech J, Rogowsky PM, Testillano PS, Rougier M, Risueno MC, Vergne P, Dumas C (2000) Genes normally expressed in the endosperm are expressed at early stages of microspore embryogenesis in maize. Plant Mol Biol 44:559–574

    Article  PubMed  CAS  Google Scholar 

  • Mogensen HL (1996) The hows and whys of cytoplasmic inheritance in seed plants. Am J Bot 83:383–404

    Google Scholar 

  • Mordhorst AP, Lörz H (1993) Embryogenesis and development of isolated barley (Hordeum vulgare L.) microspores are influenced by the amount and composition of nitrogen sources in culture media. J Plant Physiol 142:485–492

    CAS  Google Scholar 

  • Norreel B (1970) Etude cytologique de l’androgenèse expérimentale chez Nicotiania tabacum et Datura innoxia. Bull Soc Bot Fr 117:461–478

    Google Scholar 

  • Paire A, Devaux P, Lafitte C, Dumas C, Matthys-Rochon E (2003) Proteins produced by barley microspores and their derived androgenic structures promote in vitro zygotic maize embryo formation. Plant Cell Tissue Organ Cult 73:167–176

    Article  CAS  Google Scholar 

  • Pickering RA, Devaux P (1992) Haploid production: approaches and use in plant breeding. In: Shewry PR (ed) Barley: genetics, biochemistry, molecular biology and biotechnology. CAB International, Oxford, pp 519–547

    Google Scholar 

  • Raghavan V (ed) (1976a) Experimental embryogenesis in vascular plants. Academic Press, London

    Google Scholar 

  • Raghavan V (1976b) Role of generative cell in androgenesis in henbane. Science 191:388–389

    Google Scholar 

  • Salmenkallio-Marttila M, Kauppinen V (1995) Efficient regeneration of fertile plants from protoplasts isolated from microspore cultures of barley (Hordeum vulgare L.). Plant Cell Rep 14:253–256

    Article  CAS  Google Scholar 

  • Sangwan RS (1986) Formation and cytochemistry of nuclear vacuoles during meiosis in Datura. Eur J Cell Biol 40:210–218

    Google Scholar 

  • Sangwan RS, Camefort H (1982) Ribosomal bodies specific to both pollen and zygotic embryogenesis in Datura. Experientia 38:395–397

    Article  Google Scholar 

  • Sangwan RS, Camefort H (1983) The tonoplast, a specific marker of embryogenic microspores of Datura cultured in vitro. Histochemistry 78:473–480

    Article  PubMed  CAS  Google Scholar 

  • Sangwan RS, Camefort H (1984) Cold-treatment related structural modifications in the embryogenic anthers of Datura. Cytologia 49:473–487

    Google Scholar 

  • Sangwan RS, Sangwan-Norreel BS (1980) Biochemical and structural aspects of induction and development of pollen-embryos in Datura. Bull Soc Bot Fr 127:109–122

    Google Scholar 

  • Sangwan RS, Sangwan-Norreel BS (1987a) Biochemical cytology of pollen embryogenesis. Int Rev Cytol 107:221–272

    Google Scholar 

  • Sangwan RS, Sangwan-Norreel BS (1987b) Ultrastructural cytology of plastids in pollen grains of certain androgenic and nonandrogenic plants. Protoplasma 138:11–22

    Article  Google Scholar 

  • Sangwan RS, Mathivet V, Vasseur G (1989) Ultrastructural localization of acid phosphatase during male meiosis and sporogenesis in Datura: evidence for digestion of cytoplasmic structures in the vacuoles. Protoplasma 149:38–46

    Article  Google Scholar 

  • Sangwan RS, Ducrocq C, Sangwan-Norreel BS (1993) Agrobacterium-mediated transformation of pollen embryos in Datura innoxia and Nicotiana tabacum: production of transgenic haploid and fertile homozygous dihaploid plants. Plant Sci 95:99–115

    Article  CAS  Google Scholar 

  • Sangwan-Norreel BS (1978) Cytochemical and ultrastructural peculiarities of embryogenic pollen grains and of young androgenic embryos in Datura innoxia. Can J Bot 56(7):805–817

    CAS  Google Scholar 

  • Sangwan-Norreel BS (1981) Evolution in vitro du contenu en ADN nucléaire et de la ploïdie des embryons polliniques du Datura innoxia. Can J Bot 59(4):508–517

    CAS  Google Scholar 

  • Sangwan-Norreel BS (1983) Male gametophyte nuclear DNA content evolution during androgenic induction in Datura innoxia Mill. Z Pflanzenphysiol 111:47–54

    CAS  Google Scholar 

  • Shim YS, Kasha KJ (2003) The influence of pretreatment on cell stage progression and the time of DNA synthesis in barley (Hordeum vulgare L.) uninucleate microspores. Plant Cell Rep 21:1065–1071

    Article  PubMed  CAS  Google Scholar 

  • Testillano PS, Gonzalez-Melendi P, Ahmadian P, Fadon B, Risueno MC (1995) The immunolocalization of nuclear antigens during the pollen developmental programme and the induction of pollen embryogenesis. Exp Cell Res 221:41–54

    Article  PubMed  CAS  Google Scholar 

  • Testillano PS, Ramirez C, Domenech J, Coronado MJ, Vergne P, Matthys-Rochon E, Risueno MC (2002) Young microspore-derived embryos show two domains with defined features also present in zygotic embryogenesis. Int J Dev Biol 46:1035–1047

    PubMed  CAS  Google Scholar 

  • Touraev A, Vicente O, Heberle-Bors E (1997) Initiation of microspore embryogenesis by stress. Trends Plant Sci 2:297–302

    Article  Google Scholar 

  • Vazart B (1973) Formation d’embryoídes à partir de microspores de tabac: évolution de l’infrastructure des cellules au cours de la première semaine de culture. Mémoire de la Société Botanique Française, Colloque de Morphologie, Paris, pp 243–260

    Google Scholar 

  • Wang M, van Bergen S, Lamers GEM, Oppedjik BJ, Schilperroort RA (1999a) Programmed cell death during androgenesis in Hordeum vulgare L. In: Clément C, Pacini E, Audran JC (eds) Anther and pollen: from biology to biotechnology. Springer, Berlin Heidelberg New York, pp 201–209

    Google Scholar 

  • Wang M, Hoekstra S, van Bergen S, Lamers GEM, Oppedijk BJ, de Priester W, Schilperroort RA (1999b) Apoptosis in developing anthers and the role of ABA in this process during androgenesis in Hordeum vulgare L. Plant Mol Biol 39:489–501

    Article  PubMed  CAS  Google Scholar 

  • Wojnarowiez G, Jacquard C, Devaux P, Sangwan RS, Clément C (2002) Influence of copper sulfate on anther culture in barley (Hordeum vulgare L.). Plant Sci 162:843–847

    Article  CAS  Google Scholar 

  • Xie JH, Gao MW, Cai QH, Cheng X, Shen Y, Liang ZQ (1995) Improved isolated microspore culture efficiency in medium with maltose and optimized growth regulator combination in japonica rice (Oryza sativa). Plant Cell Tissue Organ Cult 42:245–250

    Article  CAS  Google Scholar 

  • Yao QA, Simion E, William M, Krochko J, Kasha KJ (1997) Biolistic transformation of haploid microspores of barley (Hordeum vulgare L.). Genome 40:570–581

    Google Scholar 

  • Zarsky V, Rihova L, Tupy J (1990) Biochemical and cytological changes in young tobacco pollen during in vitro starvation in relation to pollen embryogenesis. In: Van der Plas LHW, vanAartri JK (eds) Progress in plant cellular and molecular biology. Kluwer, Dordrecht, pp 228–233

    Google Scholar 

  • Zarsky V, Garrido D, Rihova L, Vincente O, Heberle-Bors E (1992) Derepression of the cell cycle by starvation is involved in the induction of tobacco pollen embryogenesis. Sexual Plant Reprod 4:189–194

    Google Scholar 

Download references

Author information

Authors and Affiliations

Authors

Editor information

Editors and Affiliations

Rights and permissions

Reprints and permissions

Copyright information

© 2005 Springer-Verlag Berlin Heidelberg

About this chapter

Cite this chapter

Clément, C., Sangwan, R.S., Sangwan-Norreel, B. (2005). Microspore Embryo Induction and Development in Higher Plants: Cytological and Ultrastructural Aspects. In: Don Palmer, C., Keller, W.A., Kasha, K.J. (eds) Haploids in Crop Improvement II. Biotechnology in Agriculture and Forestry, vol 56. Springer, Berlin, Heidelberg. https://doi.org/10.1007/3-540-26889-8_4

Download citation

Publish with us

Policies and ethics