Haploids in the Improvement of Linaceae and Asteraceae

  • Karin Nichterlein
  • Renate Horn
Part of the Biotechnology in Agriculture and Forestry book series (AGRICULTURE, volume 56)


Downy Mildew Anther Culture Microspore Culture Helianthus Annuus Linum Usitatissimum 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.


Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.


  1. Adda S, Reddy TP, Kishor PBK (1994) Androclonal variation in niger (Guizotia abyssinca Cass). Euphytica 79:59–64CrossRefGoogle Scholar
  2. Anonymous (2002) Growing flax — production, management and diagnostic guide, 4th edn. Flax Council of Canada and Saskatchewan Flax Development Commission, Winnipeg and SaskatoonGoogle Scholar
  3. Bachmann K, Hombergen EJ (1997) From the phenotype via QTL to virtual phenotype in Microseris (Asteraceae): predictions from multilocus marker genotypes. New Phytol 137:9–18CrossRefGoogle Scholar
  4. Badigannavar AM, Kuruvinashetti MS (1996) Callus induction and shoot bud formation from cultured anthers in sunflower (Helianthus annuus L.). Helia 19:39–46Google Scholar
  5. Berglund DR (2002) Flax: new uses and demands. In: Janick J, Whipkey A (eds) Trends in new crops and new uses. ASHS Press, Alexandria, pp 358–360Google Scholar
  6. Bergmann R, Friedt W (1997) 1. Haploidy and related biotechnological methods in linseed (Linum usitatissimum L.) In: Jain MJ, Sopory SK, Veilleux RE (eds) Current plant science and biotechnology in agriculture, vol 5. Oil, ornamental and miscellaneous plants. Kluwer, Dordrecht, pp 1–16Google Scholar
  7. Berrios EF, Gentzbittel L, Alibert G, Griveau Y, Berville A, Sarrafi A (1999) Genetic control of in vitro-organogenesis in recombinant inbred lines of sunflower (Helianthus annuus L.). Plant Breed 118:359–361CrossRefGoogle Scholar
  8. Berrios EF, Gentzbittel L, Kayal H, Alibert G, Sarrafi A (2000) AFLP mapping of QTLs for in vitro organogenesis traits using recombinant inbred lines in sunflower (Helianthus annuus L.). Theor Appl Genet 101:1299–1306Google Scholar
  9. Berry ST, Leon AJ, Hanfrey CC, Challis P, Burkholz A, Barnes SR, Rufener GK, Lee M, Caligari PDS (1995) Molecular marker analysis of Helianthus annuus L. 2. Construction of an RFLP linkage map for cultivated sunflower. Theor Appl Genet 91:195–199CrossRefGoogle Scholar
  10. Bicknell RA, Borst NK (1996) Isolation of reduced genotypes of Hieracium pilosella using anther culture. Plant Cell Tissue Organ 45:37–41Google Scholar
  11. Brahm L, Röcher T, Friedt W (2000) PCR-based markers facilitating marker assisted selection in sunflower for resistance to downy mildew. Crop Sci 40:676–682CrossRefGoogle Scholar
  12. Chen Y, Dribnenki P (2002) Effect of genotype and medium composition on flax Linum usitatissimum L. anther culture. Plant Cell Rep 21:204–207CrossRefGoogle Scholar
  13. Chen Y, Hausner G, Kenaschuk E, Procunier D, Dribnenki P, Penner G (1998a) Identification of microspore-derived plants in anther culture of flax (Linum usitatissimum L.) using molecular markers. Plant Cell Rep 18:44–48Google Scholar
  14. Chen Y, Kenaschuk E, Dribnenki P (1998b) High frequency of plant regeneration from anther culture in flax, Linum usitatissimum L. Plant Breed 117:463–467Google Scholar
  15. Chen Y, Kenaschuk, EO, Procunier D (1998c) Plant regeneration from anther culture in Canadian cultivars of flax (Linum usitatissimum L.). Euphytica 102:183–189CrossRefGoogle Scholar
  16. Chen Y, Kenaschuk E, Dribnenki P (1999) Response of flax genotypes to doubled haploid production. Plant Cell Tissue Organ Cult 57:195–198Google Scholar
  17. Chen Y, Kenaschuk E, Dribnenki P (2001) Inheritance of rust resistance genes and molecular markers in microspore-derived populations of flax. Plant Breed 120: 82–84Google Scholar
  18. Chen Y, Lin S, Duguid S, Dribnenki P, Kenaschuk E (2003) Effect of sucrose concentration on elongation of shoots from flax anther culture. Plant Cell Tissue Organ Cult 72:181–183Google Scholar
  19. Cronquist A (1988) The evolution and classification of flowering plants, 2nd edn. Allen Press, KansasGoogle Scholar
  20. Coumans M, Zhong DN (1995) Doubled haploid sunflower (Helianthus annuus) plant production by androgenesis — fact or artifact. 2. In vitro isolated microspore culture. Plant Cell Tissue Organ Cult 41:203–209CrossRefGoogle Scholar
  21. De Simon M, Morgante M, Lucchin M, Parrini P, Marcocco A (1997) A first linkage map of Cichorium intybus L. using a one-way pseudo-testcross and PCR-derived markers. Mol Breed 3:415–425Google Scholar
  22. Dore C, Pringent J, Desperez B (1996) In situ gynogenetic haploid plants of chicory (Cichorium intybus L.) after intergeneric hybridization with Cicerbita alpina Walbr. Plant Cell Rep 15:758–761Google Scholar
  23. Dribnenki JCP, Green AG (1995) Linola™ ‘947’ low linolenic acid flax. Can J Plant Sci 75:201–202Google Scholar
  24. Dribnenki JCP, Green AG, Atlin GN (1996) Linola™ 989 low linolenic flax. Can J Plant Sci 76:329–331Google Scholar
  25. Dribnenki JCP, McEachern SF, Green AG, Kenaschuk EO, Rashid KY (1999) Linola™ ‘1084’ low-linolenic acid flax. (24.1.2002)Google Scholar
  26. EC (2002) Area, yield and production of fibre flax and hemp. Directorate-General for Agriculture, European Commission. (8.10.2002)Google Scholar
  27. FAO (2003) FAOSTAT agriculture data. (4.5.2003)Google Scholar
  28. Flores-Berrios E, Gentzbittel L, Kayyal H, Alibert G, Sarrafi A (2000) AFLP mapping of QTLs for in vitro organogenesis traits using recombinant inbred lines in sunflower (Helianthus annuus L.). Theor Appl Genet 101:1299–1306Google Scholar
  29. Friedt W (1992) Present state and future prospects of biotechnology in sunflower breeding. In: Seiler G (ed) Field crops research, vol 30. Elsevier, Amsterdam, pp 425–442Google Scholar
  30. Friedt W, Bickert C, Schaub H (1995) In vitro breeding of high-linolenic, doubled-haploid lines of linseed (Linum usitatissimum L.) via androgenesis. Plant Breed 114:322–326Google Scholar
  31. Friedt W, Nurhidayah T, Röcher T, Köhler H, Bergmann R, Horn R (1997) Haploid production and application of molecular methods in sunflower (Helianthus annuus L.) In: Jain SM, Sopory SK, Veilleux RE (eds) In vitro haploid production in higher plants, vol5. Oil, ornamental and miscellaneous plants. Kluwer, Dordrecht, pp 17–35Google Scholar
  32. Gentzbittel L, Vear F, Zhang YX, Bervillé A, Nicolas P (1995) Development of a consensus linkage RFLP map of cultivated sunflower (Helianthus annuus L.). Theor Appl Genet 90:1079–1086CrossRefGoogle Scholar
  33. Gentzbittel L, Mestries E, Mouzeyar S, Mazeyrat F, Badaoui S, Vear F, Tourvieille de Labrouhe D, Nicolas P (1999) A composite map of expressed sequences and phenotypic traits of the sunflower (Helianthus annuus L.) genome. Theor Appl Genet 99:218–234CrossRefGoogle Scholar
  34. Green AG, Marshall DR (1984) Isolation of induced mutants in linseed (Linum usitatissimum) having reduced linolenic acid content. Euphytica 33:321–328CrossRefGoogle Scholar
  35. Hahne G (2001) Sunflower. In: Hui YH, Khatchtourians GG, McHughen A, Nip WK, Scorza R (eds) Handbook of transgenic plants. Dekker, New York, pp 813–833Google Scholar
  36. Hewezi T, Jardinaud F, Alibert G, Kallerhoff J (2003) A new approach for efficient regeneration of a recalcitrant genotype of sunflower (Helianthus annuus) by organogenesis induction on split embryonic axes. Plant Cell Tissue Organ Cult 73:81–86CrossRefGoogle Scholar
  37. Ivanov P, Encheva J, Ivanova I (1998) A protocol to avoid precocious flowering of sunflower plantlets in vitro. Plant Breed 117:582–584Google Scholar
  38. Jeuken M, van Wijk R, Peleman J, Lindhout P (2001) An integrated interspecific AFLP map of lettuce (Lactuca) based on two L. sativa × L. saligna F2-populations. Theor Appl Genet 103:638–647CrossRefGoogle Scholar
  39. Kessli RV, Paran I, Michelmore RW (1994) Analysis of a detailed genetic-linkage map of Lactuca sativa (lettuce) constructed from RFLP and RAPD markers. Genetics 136:1435–1446Google Scholar
  40. Korell M, Brahm L, Horn R, Friedt W (1996a) Interspecific and intergeneric hybridization in sunflower breeding. I. General breeding aspects. Plant Breed Abstr 66:925–931Google Scholar
  41. Korell M, Brahm L, Friedt W, Horn R (1996b) Interspecific and intergeneric hybridization in sunflower breeding. II. Specific uses of wild germplasms. Plant Breed Abstr 66:1081–1091Google Scholar
  42. Kurt O, Evans GM (1998) Anther culture potential of linseed (Linum usitatissimum L.): effects of genotypes and pretreatment on callus formation and differentiation. J Agric For 22:553–560Google Scholar
  43. Lawson WR, Goulter KC, Henry RJ, Kong GA, Kochmann JK (1998) Marker-assisted selection for two rust resistance genes in sunflower. Mol Breed 4:227–234CrossRefGoogle Scholar
  44. Leon AJ, Lee M, Rufener GK, Berry ST, Movers RP (1995) Use of RFLP markers for genetic linkage analysis of oil percentage in sunflower seeds. Crop Sci 35:558–564CrossRefGoogle Scholar
  45. Leon AJ, Lee M, Andrade FH (2001) Quantitative trait loci for growing degree days to flowering and photoperiod response in sunflower (Helianthus annuus L.). Theor Appl Genet 102:497–503Google Scholar
  46. Lühs W, Friedt W (1994) The major oil crops. In: Murphy DJ (ed) Designer oil crops. VCH, Weinheim, pp 1–130Google Scholar
  47. Manninen OM (2000) Associations between anther-culture response and molecular markers on chromosome 2H, 3H, and 4H of barley (Hordeum vulgare L). Theor Appl Genet 100:57–62CrossRefGoogle Scholar
  48. Mouzeyar S, Roeckel-Drevet P, Gentzbittel L, Philippon J, Tourvieille de Labrouhe D, Vear F, Nicolas P (1995) RFLP and RAPD mapping of the sunflower Pl1 locus for resistance to Plasmopara halstedii race 1. Theor Appl Genet 91:733–737CrossRefGoogle Scholar
  49. Nenova N, Christov M, Ivanov P (2000) Anther culture regeneration from some wild Helianthus species. Helia 23:65–72Google Scholar
  50. Nestares G, Zorzoli R, Mroginski L, Picardi L (2002) Heritability of in vitro regeneration capacity in sunflower. Plant Breed 121:366–368CrossRefGoogle Scholar
  51. Nichterlein K (2003) Anther culture of linseed (Linum usitatissimum L.). In: Maluszynski M, Kasha, KJ, Forster BP, Szarekjo I (eds) Doubled haploid production in crop plants. A manual. Kluwer, Dordrecht, pp 1–480Google Scholar
  52. Nichterlein K, Friedt W (1993) Plant regeneration from isolated microspores of linseed (Linum usitatissimum L.). Plant Cell Rep 12:426–430CrossRefGoogle Scholar
  53. Nichterlein K, Nickel M, Umbach H, Friedt W (1989): Recent progress and prospects of biotechnology in breeding of linseed (Linum usitatissimum). Fat Sci Technol 91:272–275Google Scholar
  54. Nichterlein K, Umbach H, Friedt, W (1991) Genotypic and exogenous factors affecting shoot regeneration from anther callus of linseed (Linum usitatissimum L.). Euphytica 58:157–164CrossRefGoogle Scholar
  55. Nurhidayah T, Horn R, Röcher T, Friedt W (1996) High regeneration rates in anther culture of interspecific sunflower hybrids. Plant Cell Rep 16:167–173Google Scholar
  56. Prairie Registration Recommending Committee for Grains (2002) PRRCG report. Oilseeds subcommittee. New lines recommended. Flax. 13. Meristem land and science. Scholar
  57. Prasad BR, Khadeer MA, Seeta P, Anwar SY (1990) Influence of genotype and cold pretreatment on anther culture response in safflower, Carthamus tinctorius L. Indian J Exp Biol 28:924–927Google Scholar
  58. Prasad BR, Khadeer MA, Seeta P, Anwar SY (1991) In vitro induction of androgenic haploids in safflower (Carthamus tinctorius L.). Plant Cell Rep 10:48–51Google Scholar
  59. Pretova A, Obert B (2000) Progress in flax embryogenesis. In: Bohanec B (ed) Proc COST 824 Final Meeting, Biotechnological Approaches for Utilization of Gametic Cells, Bled, Slovenia 1–5 July. Directorate General for Research, European Commission, pp 165–169Google Scholar
  60. Rieseberg L, Coi H, Chan R, Spore C (1993) Genomic map of a diploid hybrid species. Heredity 70:285–293Google Scholar
  61. Saji KV, Sujatha M (1998) Embryogenesis and plant regeneration in anther culture of sunflower (Helianthus annuus L.). Euphytica 103:1–7CrossRefGoogle Scholar
  62. Sarrafi A, Roustan JP, Fallot J, Alibert G (1996) Genetic analysis of organogenesis in the cotyledons of sunflower (Helianthus annuus L.). Theor Appl Genet 92:225–229Google Scholar
  63. Schilling EE, Heiser CB (1981) Intrageneric classification of Helianthus (Compositae). Taxon 30:393–403Google Scholar
  64. Spielmeyer W, Green AG, Bittisnich D, Mendham N, Lagudah ES (1998a) Identification of quantitative trait loci contributing to Fusarium wilt resistance on an AFLP linkage map of flax (Linum usitatissimum). Theor Appl Genet 97:633–641CrossRefGoogle Scholar
  65. Spielmeyer W, Lagudah ES, Mendham N, Green AG (1998b) Inheritance of resistance to flax wilt (Fusarium oxysporum f.sp. Schlecht) in a doubled haploid population of Linum usitatissimum L. Euphytica 101:287–291CrossRefGoogle Scholar
  66. Steiss R, Schuster A, Friedt W (1998) Development of linseed for industrial purposes via pedigree-selection and haploid-technique. Ind Crop Prod 7:303–309CrossRefGoogle Scholar
  67. Tang S, Yu JK, Slabaugh MB, Shintami DK, Knapp SJ (2002) Simple sequence repeat map of the sunflower genome. Theor Appl Genet 105:1124–1136PubMedCrossRefGoogle Scholar
  68. Theiler R, Hunter CS (1995) Regeneration of dihaploid chicory (Cichorium intybus L. varfoliosum hegi) via microspore culture. Plant Breed 114:18–23Google Scholar
  69. Theiler R, Lüscher M, Hunter CS (1997) Differentiation between diploid and doubled haploid chicory (Cichorium intybus L.) by RAPD-PCR analysis. Acta Hortic 447:367–370Google Scholar
  70. Todorova M, Ivanov P (1999) Induced parthenogenesis in sunflower: effect of pollen donor. Helia 22:49–56Google Scholar
  71. Todorova M, Nenova N, Ivanov P, Christov M (1997a) Plant regeneration through anther culture and induced parthenogenesis in the genus Helianthus. Biotechnol Equip 11:A27–A30Google Scholar
  72. Todorova M, Ivanov P, Shindrova P, Christov M, Ivanov I (1997b) Doubled haploid production of sunflower (Helianthus annuus L.) through irradiated pollen-induced parthenogenesis. Euphytica 97:249–254CrossRefGoogle Scholar
  73. Vanhouten W, Vanraamsdonk L, Bachmann K (1994) Intraspecific evolution of Microseris pygmaea (Asteraceae, Lactuceae) analysed by cosegregation of phenotypic characters (QTLs) and molecular markers (RAPDs). Plant Syst Evol 190:49–67Google Scholar
  74. Vasic D, Skoric D, Jocic S (2000) Anther culture of sunflower cultivars. In: Proc 15th Int Sunflower Conf, Toulouse, France 12–15 June, L52–L55Google Scholar
  75. Varotto S, Luccin M, Parrini P (1996) Anther culture in Cichorium intybus L. Italus-Hortus 3:12–15Google Scholar
  76. Waycott W, Fort SB, Ryder EJ, Michelmore RW (1999) Mapping morphological genes relative to molecular markers in lettuce (Lactuca sativa L.). Heredity 82:245–251PubMedCrossRefGoogle Scholar
  77. Witsenboer H, Vogel J, Michelmore RW (1997) Identification, genetic localization, and allelic diversity of selectively amplified microsatellite polymorphic loci in lettuce and wild relatives (Lactuca spp.). Genome 40:923–936PubMedGoogle Scholar
  78. Weber S, Horn R, Friedt W (2000) High regeneration potential in vitro of sunflower (Helianthus annuus L.). Plant Cell Rep 116:271–280Google Scholar
  79. Xu Y, Zhu L, Xiao J, Huang N, McCouch SR (1997) Chromosomal regions associated with segregation distortion of molecular markers in F2, backcross, doubled haploid, and recombinant inbred populations in rice (Oryza sativa L.). Mol Gen Genet 253:535–545PubMedGoogle Scholar
  80. Yang HY, Yan H, Zhou C (1995) In vitro production of haploids in Helianthus. In: Bajaj YPS (ed) Biotechnology in agriculture and forestry, vol 10. Legumes and oilseed crops. Springer, Berlin Heidelberg New York, pp 472–486Google Scholar
  81. Yu JK, Tang S, Slabaugh MB, Heesacker A, Cole G, Herring M, Soper J, Han F, Chu WC, Webb DM, Thompson L, Edwards KJ, Berry S, Leon AJ, Grondona M, Olungu C, Maes N, Knapp SL (2003) Towards a saturated molecular genetic linkage map for cultivated sunflower. Crop Sci 43:367–387CrossRefGoogle Scholar
  82. Zhong D, Michauxferriere N, Coumans M (1995) Assay for doubled haploid sunflower (Helianthus annuus) plant production by androgenesis. Fact or artifact. 1. In vitro anther culture. Plant Cell Tissue Organ Cult 41:91–97CrossRefGoogle Scholar
  83. Zivy M, Devaux P, Blaisonneaux J, Jean RTH (1992) Segregation distortion and linkage studies in microspore-derived double haploid lines of Hordeum vulgare L. Theor Appl Genet 83:919–924CrossRefGoogle Scholar

Copyright information

© Springer-Verlag Berlin Heidelberg 2005

Authors and Affiliations

  • Karin Nichterlein
    • 1
  • Renate Horn
    • 2
  1. 1.Joint FAO/IAEA Division of Nuclear Techniques in Food and AgricultureViennaAustria
  2. 2.Department of Genetics and BiochemistryClemson UniversityClemsonUSA

Personalised recommendations