Epicutaneous Sensitization with Allergens as an Atopic Dermatitis Model
Relevant animal models of human diseases are invaluable. The murine model described here incorporates several features that are likely to be important in the pathogenesis of atopic dermatitis in humans. EC sensitization with protein allergens induces dermatitis manifested by epidermal and dermal thickening and characterized by the dermal accumulation of CD4+ T cells, eosinophils, and degranulated mast cells. In addition, allergen-sensitized skin reveals an accumulation of Th2 cytokine mRNA. Finally, EC allergen sensitization in the absence of adjuvant leads to increased levels of total serum IgE and allergen-specific IgE as well.
The availability of this model system should facilitate the investigation of the molecular and cellular mechanisms of allergen-induced skin inflammation. Moreover, the effects of trigger factors of AD (e.g., skin injury and superantigens) on the development of skin inflammation and antibody responses can be examined. Finally, efficacy of novel topical and systemic therapies that might be predicted to have efficacy in humans can be carefully tested.
KeywordsAtopic Dermatitis Skin Inflammation Allergy Clin Immunol Natural Rubber Latex Skin Site
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