Making Gene-Modified Mice
Current advances in the technologies available for the manipulation of the mouse genome have revolutionised experimental biology and have provided an unprecedented tool for the in vivo analysis of gene function in mammals. Studies in gene-modified mice have made a major contribution to our current understanding of the molecular mechanisms governing mammalian embryonic development and physiology. Furthermore, this technology has allowed the generation of mouse models of numerous human genetic diseases, thus providing an in vivo experimental system for the study of the molecular and cellular mechanisms of disease pathogenesis. The application and further improvement of this technology in combination with current developments facilitating large-scale genome sequencing and annotation and the high-throughput analysis of gene expression and protein complex formation and function will undoubtedly play a central role in biomedical research in the years to come, hopefully leading to the better understanding of pathogenic mechanisms and to new, more effective therapeutic approaches to human disease.
KeywordsBacterial Artificial Chromosome Homologous Recombination Selection Marker Embryonic Stem Cell Line loxP Site
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