Molecular Imaging with Targeted Ultrasound Contrast Microbubbles
Microbubbles were prepared from insoluble perfluorocarbon gas, stabilized with a monolayer of phospholipid with a grafted PEG brush and decorated with targeting ligands, such as monoclonal antibodies or peptides on a flexible PEG spacer arm. Such targeted bubbles selectively and firmly bind to the surfaces coated with the specific receptors in vitro. Bubble deposition areas can be detected by ultrasound imaging. Single bubble detection sensitivity is possible.
Targeting of microbubbles to areas of inflammation, ischemia-reperfusion injury, and angiogenesis (including tumors) was achieved in vivo in experimental animal studies, by the use of microbubbles directed towards P-selectin or α v/β 3, respectively. Selective accumulation of ligand-carrying bubbles in the tissues of interest was confirmed by fluorescence microscopy (intravital and confocal) and ultrasound imaging.
KeywordsBubble Surface Ultrasound Contrast Agent Myocardial Contrast Echocardiography Ultrasound Contrast Material Contrast Particle
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