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Molecular Imaging with Targeted Ultrasound Contrast Microbubbles

  • A. L. Klibanov
Part of the Ernst Schering Research Foundation Workshop book series (SCHERING FOUND, volume 49)

10.6 Conclusions

Microbubbles were prepared from insoluble perfluorocarbon gas, stabilized with a monolayer of phospholipid with a grafted PEG brush and decorated with targeting ligands, such as monoclonal antibodies or peptides on a flexible PEG spacer arm. Such targeted bubbles selectively and firmly bind to the surfaces coated with the specific receptors in vitro. Bubble deposition areas can be detected by ultrasound imaging. Single bubble detection sensitivity is possible.

Targeting of microbubbles to areas of inflammation, ischemia-reperfusion injury, and angiogenesis (including tumors) was achieved in vivo in experimental animal studies, by the use of microbubbles directed towards P-selectin or α v/β 3, respectively. Selective accumulation of ligand-carrying bubbles in the tissues of interest was confirmed by fluorescence microscopy (intravital and confocal) and ultrasound imaging.

Keywords

Bubble Surface Ultrasound Contrast Agent Myocardial Contrast Echocardiography Ultrasound Contrast Material Contrast Particle 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

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Copyright information

© Springer-Verlag Berlin Heidelberg 2005

Authors and Affiliations

  • A. L. Klibanov
    • 1
  1. 1.Cardiovascular DivisionUniversity of Virginia Medical CenterCharlottesvilleUSA

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