Coronavirus Reverse Genetics by Targeted RNA Recombination

  • P. S. Masters
  • P. J. M. Rottier
Part of the Current Topics in Microbiology and Immunology book series (CT MICROBIOLOGY, volume 287)


Targeted RNA recombination was the first reverse genetics system devised for coronaviruses at a time when it was not clear whether the construction of full-length infectious cDNA clones would become possible. In its current state targeted RNA recombination offers a versatile and powerful method for the site-directed mutagenesis of the downstream third of the coronavirus genome, which encodes all the viral structural proteins. The development of this system is described, with an emphasis on recent improvements, and multiple applications of this technique to the study of coronavirus molecular biology and pathogenesis are reviewed. Additionally, the relative strengths and limitations of targeted RNA recombination and infectious cDNA systems are contrasted.


Infectious Bronchitis Virus Mouse Hepatitis Virus Reverse Genetic System Defective Interfere Murine Coronavirus 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.


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Copyright information

© Springer-Verlag 2005

Authors and Affiliations

  • P. S. Masters
    • 1
  • P. J. M. Rottier
    • 2
  1. 1.Laboratory of Viral Disease, Division of Infectious Disease, Wadsworth CenterNew York State Department of HealthAlbanyUSA
  2. 2.Virology Division, Department of Infectious Diseases and Immunology, Faculty of Veterinary Medicine and Institute of BiomembranesUtrecht UniversityUtrechtThe Netherlands

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