The Use of Microfluidic Techniques in Microarray Applications

  • Piotr Grodzinski
  • Robin H. Liu
  • Ralf Lenigk
  • Yingjie Liu
Part of the Biological and Medical Physics, Biomedical Engineering book series (BIOMEDICAL)


Total Analysis System High Density Array Hybridization Kinetic Hybridization Channel Microarray Application 
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  1. 1.
    Fodor, S. P., Read, J. L., Pirrung, M. C., Stryer, L., Lu, A. T., Solas, D. (1991) Light-directed, spatially addressable parallel chemical synthesis. Science 251, 767–773PubMedGoogle Scholar
  2. 2.
    Schena, M. Microarray BiochipTechnology. Eaton Publishing: Natick, MA, 2000Google Scholar
  3. 3.
    Duggan, D. J., Bittner, M., Chen, Y., Meltzer, P., and Trent, J. M. (1999) Expression profiling using cDNA microarrays. Nature Genetics 21, 10–14PubMedCrossRefGoogle Scholar
  4. 4.
    Brown, P. O., and Botstein, D. (1999) Exploring the new world of the genome with DNA microarrays. Nature Genetics 21, 33–37PubMedCrossRefGoogle Scholar
  5. 5.
    Debouck, C., and Goodfellow, P. N. (1999) DNA microarrays in drug discovery and development. Nature Genetics 21, 48–50PubMedCrossRefGoogle Scholar
  6. 6.
    Hacia, J. G. (1999) Resequencing and mutational analysis using oligonucleotide microarrays. Nature Genetics 21, 42–47PubMedCrossRefGoogle Scholar
  7. 7.
    Liu, R. H., Grodzinski, P. In Microfluidic and BioMEMS Applications; Tay, F. E. H., Ed.; Kluwer Academic Publishers: Boston, 2002, pp 143–184Google Scholar
  8. 8.
    Wittwer, C. T., Fillmore, G.C., Garling, D.J. (1990) Minimizing the time required for DNA amplification by efficient heat transfer to small samples. Anal Biochem 186, 328–331PubMedCrossRefGoogle Scholar
  9. 9.
    Belgrader, P., Benett, W., Hadley, D., Richards, J., Stratton, P., Mariella Jr., R., Milanovich, F., (1999) PCR detection of bacteria in seven minutes. Science, 284, 449–450PubMedCrossRefGoogle Scholar
  10. 10.
    Hühmer, A. F. R. (2000) Noncontact infrared-mediated thermocycling for effective polymerase chain reaction amplification of DNA in nanoliter volumes. Analytical Chemistry 72, 5507–5512PubMedCrossRefGoogle Scholar
  11. 11.
    Southern, E., Mir, K., and Shchepinov, M. (1999) Molecular interactions on microarrays. [Review] [37 refs]. Nature Genetics 21, 5–9PubMedCrossRefGoogle Scholar
  12. 12.
    Edman, C. F., Raymond, D. E., Wu, D. J., Tu, E., Sosnowski, R. G., Butler, W. F., Nerenberg, M., and Heller, M. J. (1997) Electric field directed nucleic acid hybridization on microchips. Nucleic Acids Res 25, 4907–14PubMedCrossRefGoogle Scholar
  13. 13.
    Radtkey, R., Feng, L., Muralhidar, M., Duhon, M., Canter, D., DiPierro, D., Fallon, S., Tu, E., McElfresh, K., Nerenberg, M., and Sosnowski, R. (2000) Rapid, high fidelity analysis of simple sequence repeats on an electronically active DNA microchip. Nucleic Acids Res 28, E17PubMedCrossRefGoogle Scholar
  14. 14.
    Fan ZH, M. S., Granzow R, Heaney P, Ho W, Dong Q, Kumar R. (1999) Dynamic DNA hybridization on a chip using paramagnetic beads. Anal Chem 71, 4851–4859PubMedCrossRefGoogle Scholar
  15. 15.
    Fan, Z. H., Kumar, R. (2001) In Biochip Technology, Cheng, J., Kricka, L., Ed., Harwood Academic Publishers, Philadelphia, PA, pp. 291–307Google Scholar
  16. 16.
    Chee, M., Yang, R., Hubbell, E., Berno, A., Huang, X. C., Stern, D., Winkler, J., Lockhart, D. J., Morris, M. S., and Fodor, S. P. (1996) Accessing genetic information with high-density DNA arrays. Science 274, 610–4PubMedCrossRefGoogle Scholar
  17. 17.
    Cheek, B.J., Steel, A.B., Torres, M.P., Yu, Y-Y., Yang, H. (2001) Chemiluminescence Detection for Hybridization Assays on the Flow-Thru Chip, a Three-Dimensional Microchannel Biochip. Anal Chem 73, 5777–5783PubMedCrossRefGoogle Scholar
  18. 18.
    Liu, R. H., Chen, H., Luehrsen, K. R., Ganser, D., Weston, D., Blackwell, J., Grodzinski, P., In Technical Digest of International MEMS Conf., Interlaken, Switzerland, Jan. 21–25 2001; 439–442Google Scholar
  19. 19.
    Liu, R., Lenigk, R., Luehrsen, K. R., Yu, H., Chen, H., Ganser, D., Bonanno, J., Grodzinski, P., In Micro Total Analysis Systems 2001; Ramsey, J. M., Van den Berg, A., Eds.; Kluwer Academic Publishers: Monterey, CA, 2001, 465–467Google Scholar
  20. 20.
    Lenigk, R., Liu, R., Athavale, M., Chen, Z., Ganser, D., Yang, J., Rauch, C., Liu, Y., Chan, B., Yu, H., Ray, M., Marrero, R., Grodzinski, P. (2002) Plastic biochannel hybridization devices: A new concept for microfluidic DNA arrays. Analytical Biochemistry 311, 40–49PubMedCrossRefGoogle Scholar
  21. 21.
    Jackman, R. J., Brittain, S. T., Adams, A., Prentiss, M. G., Whitesides, G. (1998) Design and Fabrication of Topologically Complex, Three-Dimensional Microstructures. Science 280, 2089–2091PubMedCrossRefGoogle Scholar
  22. 22.
    Jo, B. H., Lerberghe, L. M. V., Motsegood, K. M., Beebe, D. J. (2000) Three-dimensional micro-channel fabrication in Polydimethylsiloxane(PDMS) elastomer. Journal of Micro-electro-mechanical Systems 9, 76–81Google Scholar
  23. 23.
    Umek RM, L. S., Vielmetter J, Terbrueggen RH, Irvine B, Yu CJ, Kayyem JF, Yowanto H, Blackburn GF, Farkas DH, Chen YP. (2001) Electronic detection of nucleic acids: a versatile platform for molecular diagnostics J Mol Diagn 3, 74–84PubMedGoogle Scholar
  24. 24.
    Steel, A., Torres, M., Hartwell, J., Yu, Y.-Y., Ting, N., Hoke, G., Yang, H. In Microarray Biochip Technology; Schena, M., Ed.; Eaton Publishing, 2000, pp 87–117Google Scholar
  25. 25.
    Corporation, C. R. (2001) CFD-ACE+ User's Manual, CFD Research Corporation, Huntsville, ALGoogle Scholar
  26. 26.
    Elder, S. A. (1959) Cavitation Microstreaming. J. Acoust. Soc. Am. 31, 54–62CrossRefGoogle Scholar
  27. 27.
    Liu, R.H., Lenigk, R., Ganser, D., Bonanno, J., Sanchez, B., Singal, P., Grodzinski, P., Improvement of DNA Microarray Biochips using Microfluidic Mixing Technique, presented at Solid-State Sensor and Actuator Workshop, Hilton Head Island, SC, June, 2002Google Scholar
  28. 28.
    Liu, R. H., Lenigk, R., Yang, J., Druyor-Sanchez, R., Singal, P., Grodzinski, P. (2003) Hybridization Enhancement by Bubble-induced Acoustic Microstreaming. Analytical Chemistry, 75 1911–1917PubMedCrossRefGoogle Scholar
  29. 29.
    Nyborg, W. L. (1958) Acoustic streaming near a boundary. J. Acoust. Soc. Am. 30, 329–339CrossRefGoogle Scholar
  30. 30.
    Kolb, J., Nyborg, W. L. (1956) Small-Scale Acoustic Streaming in Liquids. J. Acoust. Soc. Am. 28, 1237–1242CrossRefGoogle Scholar
  31. 31.
    Bard, A. J., Faulkner, L. R. Electrochemical Methods; Wiley: New York, 1980Google Scholar
  32. 32.
    White, F. M. Viscous Fluid Flow; McGraw-Hill: New York, 1974Google Scholar
  33. 33.
    Jonsson, U. L., al., e. (1991) Real-time biospecific interaction analysis using surface plasmon resonance and a sensor chip technology. BioTechniques 11, 620–627PubMedGoogle Scholar
  34. 34.
    Garland, P. B. (1996) Optical evanescent wave methods for the study of biomolecular interactions. Q. Rev. Biophys. 29, 91–117PubMedCrossRefGoogle Scholar
  35. 35.
    Anderson, R. C., Su, X., Bogdan, G. J., Fenton, J. (2000) A miniature integrated device for automated multistep genetic assays. Nucleic Acids Res 28, E60PubMedCrossRefGoogle Scholar
  36. 36.
    Harrison, D. J., Fluri, K., Seiler, K., Fan, Z., Effenhauser, C. S., Manz, A. (1993) Micromachining a Miniaturized Capillary Electrophoresis-based Chemical Analysis System on a Chip. Science 261, 895–897PubMedGoogle Scholar
  37. 37.
    Wang, J. (2000) From DNA biosensors to gene chips. Nucleic Acids Res 28, 3011–3016PubMedCrossRefGoogle Scholar
  38. 38.
    Ramsey, J. M., Jacobson, S. C., Knapp, M. R. (1995) Microfabricated Chemical Measurement Systems. Nature Medicine 1, 1093–1096PubMedCrossRefGoogle Scholar
  39. 39.
    Jacobson, S. C., Hergenroder, R., Moore Jr., A. W., Ramsey, J. M. (1994) Precolumn Reactions with Electrophoretic Analysis Integrated on a Microchip. Anal. Chem. 66, 4127–4132CrossRefGoogle Scholar
  40. 40.
    Woolley, A. T., Hadley, D., Landre, P., deMello, A. J., Mathies, R. A., Northrup, M. A. (1996) Functional Integration of PCR Amplification and Capillary Electrophoresis in a Microfabricated DNA Analysis Device. Anal. Chem. 68, 4081–4086PubMedCrossRefGoogle Scholar
  41. 41.
    Waters, L. C., Jacobson, S. C., Kroutchinina, N., Khandurina, J., Foote, R. S., Ramsey, J. M. (1998) Microchip Device for Cell Lysis, Multiplex PCR Amplification, and Electrophoretic Sizing. Anal. Chem. 70, 158–162PubMedCrossRefGoogle Scholar
  42. 42.
    Burns, M. A., Johnson, B. N., Brahmasandra, S. N., Handique, K., Webster, J. R., Krishnan, M., Sammarco, T. S., Man, P. M., Jones, D., Heldsinger, D., Mastrangelo, C. H., Burke, D. T. (1998) An integrated nanoliter DNA analysis device. Science 282, 484–487PubMedCrossRefGoogle Scholar
  43. 43.
    Sosnowski, R. G., Tu, E., Butler, W. F., O'Connell, J. P., Heller, M. J. (1997) Rapid determination of single base mismatch mutations in DNA hybrids by direct electric field control. Proc Natl Acad Sci U S A 94, 1119–1123PubMedCrossRefGoogle Scholar
  44. 44.
    Taylor, M. T., Belgrader, P., Joshi, R., Kintz, G. A., Northrup, M. A. In Micro Total Analysis Systems 2001; Ramsey, J. M., Van den Berg, A., Eds.; Kluwer Academic Publishers: Monterey, CA, 2001, pp 670–672Google Scholar
  45. 45.
    Yuen, P., Kricka, L., Fortina, P., Panaro, N., Sakazume, T., Wilding, P. (2001) Microchip Module for Blood Sample Preparation and Nucleic Acid Amplification Reactions. Genome Research 11, 405–412PubMedCrossRefGoogle Scholar
  46. 46.
    Smith, L., Hok, B., In Transducers '91, San Francisco, CA 1991; 1049–1051Google Scholar
  47. 47.
    Barth, P. W. In Transducers'95: Stockholm, Sweden, 1995, pp 276–277Google Scholar
  48. 48.
    Jacobson, S. C., McKnight, T. E., Ramsey, J. M. (1999) Microfluidic Devices for Electrokinetically Driven Parallel and Serial Mixing. Analytical Chemistry 71, 4455–4459CrossRefGoogle Scholar
  49. 49.
    Duffy, D. C., Gillis, H. L., Lin, J., Sheppard, N. F., Kellogg, G. L. (1999) Microfabricated Centrifugal Microfluidic Systems: Characterization and Multiple Enzymatic Assays. Analytical Chemistry 71, 4669–4678CrossRefGoogle Scholar
  50. 50.
    Unger, M. A., Chou, H. P., Thorsen, T., Scherer, A., Quake, S. R. (2000) Monolithic microfabricated valves and pumps by multilayer soft lithography. Science 288, 113–116PubMedCrossRefGoogle Scholar
  51. 51.
    Peters, E. C., Svec, F., Frechet, J. M. J. (1999) Rigid Macroporous Polymer Monoliths. Advanced Materials 11, 1169–1181CrossRefGoogle Scholar
  52. 52.
    Beebe, D. J., Moore, J. S., Bauer, J. M., Yu, Q., Liu, R. H., Devadoss, C., Jo, B. H. (2000) Functional hydrogel structures for autonomous flow control inside microfluidic channels. Nature 404, 588–590PubMedCrossRefGoogle Scholar
  53. 53.
    Liu, Y., Rauch, C., Stevens, R., Lenigk, R., Yang, J., Rhine, D., Grodzinski, P. (2002) DNA Amplification and Hybridization Assays in Integrated Plastic Monolithic Devices. Analytical Chemistry 74, 3063–3070PubMedCrossRefGoogle Scholar
  54. 54.
    Chiou, J., Matsudaira, P., Sonin, A., Ehrlich, D. (2001) A closed-cycle capillary polymerase chain reaction machine. Anal Chem 73, 2018–2021PubMedCrossRefGoogle Scholar

Copyright information

© Springer-Verlag Berlin Heidelberg 2005

Authors and Affiliations

  • Piotr Grodzinski
  • Robin H. Liu
  • Ralf Lenigk
  • Yingjie Liu

There are no affiliations available

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