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Preparation of Endothelial Cells from Microand Macrovascular Origin

  • Saskia C. Peters
  • Anna Reis
  • Thomas Noll

Keywords

Perfusion System Endothelial Cell Culture Newborn Calf Serum Cell Culture Technique Amanita Phalloides 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

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References

  1. Adamson RH, Curry FE, Adamson G, Liu B, Jiang Y, Aktories K, Barth H, Daigeler A, Golenhofen N, Ness W and Drenckhahn D (2002): Rho and Rho Kinase Modulation of Barrier Properties: Cultured Endothelial Cells and Intact Microvessels of Rats and Mice. J Physiol 539: 295–308CrossRefPubMedGoogle Scholar
  2. Bahnak BR, Wu QY, Coulombel L, Assouline Z, Kerbiriou-Nabias D, Pietu G, Drouet L, Caen JP and Meyer D (1989): Expression of von Willebrand Factor in Porcine Vessels: Heterogeneity at the Level of von Willebrand Factor mRNA. J Cell Physiol 138: 305–310CrossRefPubMedGoogle Scholar
  3. Booyse FM, Sedlak BJ and Rafelson ME, Jr (1975): Culture of Arterial Endothelial Cells: Characterization and Growth of Bovine Aortic Cells. Thromb Diath Haemorrh 34: 825–839PubMedGoogle Scholar
  4. Bowman PD, Betz AL, Ar D, Wolinsky JS, Penney JB, Shivers RR and Goldstein GW (1981): Primary Culture of Capillary Endothelium from Rat Brain. In Vitro 17: 353–362PubMedGoogle Scholar
  5. Bowman PD, Betz AL and Goldstein GW (1982): Primary Culture of Microvascular Endothelial Cells from Bovine Retina: Selective Growth using Fibronectin Coated Substrate and Plasma Derived Serum. In Vitro 18: 626–632PubMedGoogle Scholar
  6. Cirillo P, Golino P, Ragni M, Guarino A, Calabro P and Chiarriello M (1999): A Simple Method for the Isolation, Cultivation, and Characterization of Endothelial Cells from Rabbit Coronary Circulation. Thromb Res 96: 329–333CrossRefPubMedGoogle Scholar
  7. Davison PM, Bensch K and Karasek MA (1980): Isolation and Growth of Endothelial Cells from the Microvessels of the Newborn Human Foreskin in Cell Culture. J Invest Dermatol 75: 316–321CrossRefPubMedGoogle Scholar
  8. Dong QG, Bernasconi S, Lostaglio S, De Calmanovici RW, Martin-Padura I, Breviario F, Garlanda C, Ramponi S, Mantovani A and Vecchi A (1997): A General Strategy for Isolation of Endothelial Cells from Murine Tissues: Characterization of Two Endothelial Cell Lines from the Murine Lung and Subcutaneous Sponge Implants. Arterioscler Thromb Vasc Biol 17: 1599–1604PubMedGoogle Scholar
  9. Draijer R, Vaandrager AB, Nolte C, de Jonge HR, Walter U and van Hinsbergh VW (1995): Expression of cGMP-Dependent Protein Kinase I and Phosphorylation of its Substrate, Vasodilator-Stimulated Phosphoprotein, in Human Endothelial Cells of Different Origin. Circ Res 77: 897–905PubMedGoogle Scholar
  10. Faulstich H, Zobeley S, Heintz D and Drewes G (1993): Probing the Phalloidin Binding Site of Actin. FEBS Lett 318:218–222CrossRefPubMedGoogle Scholar
  11. Fitzgerald U, Hettle S, MacDonald C and McLean JS (2000): Umbilical Cord Endothelial Cells Expressing Large T Antigen: Comparison with Primary Cultures and Effect of Cell Age. In Vitro Cell Dev Biol Anim 36: 222–227CrossRefPubMedGoogle Scholar
  12. Gräfe M, Auch-Schwelk W, Graf K, Terbeek D, Hertel H, Unkelbach M, Hildebrandt A and Fleck E (1994): Isolation and Characterization of Macrovascular and Microvascular Endothelial Cells from Human Hearts. Am J Physiol 267:H2138–H2148PubMedGoogle Scholar
  13. Hewett PW and Murray JC (1993): Human Lung Microvessel Endothelial Cells: Isolation, Culture, and Characterization. Microvasc Res 46: 89–102CrossRefPubMedGoogle Scholar
  14. Hewett PW, Murray JC, Price EA, Watts ME and Woodcock M (1993): Isolation and Characterization of Microvessel Endothelial Cells from Human Mammary Adipose Tissue. In Vitro Cell Dev Biol Anim 29A: 325–331PubMedGoogle Scholar
  15. Jaffe EA, Nachman RL, Becker CG and Minick CR (1973): Culture of Human Endothelial Cells Derived from Umbilical Veins: Identification by Morphologic and Immunologic Criteria. J Clin Invest 52: 2745–2756PubMedGoogle Scholar
  16. Low I, and Wieland T (1974): The Interaction of Phalloidin: Some of its Derivatives, and of other Cyclic Peptides with Muscle Actin as Studied by Viscosimetry. FEBS Lett 44: 340–343PubMedGoogle Scholar
  17. Magee JC, Stone AE, Oldham KT and Guice KS (1994): Isolation, Culture, and Characterization of Rat Lung Microvascular Endothelial Cells. Am J Physiol 267: L433–L441PubMedGoogle Scholar
  18. Mischeck U, Meyer J and Galla HJ (1989): Characterization of Gamma-Glutamyl Transpeptidase Activity of Cultured Endothelial Cells from Porcine Brain Capillaries. Cell Tissue Res 256: 221–226CrossRefPubMedGoogle Scholar
  19. Nees S, Gerbes AL, Gerlach E and Staubesand J (1981): Isolation, Identification, and Continuous Culture of Coronary Endothelial Cells from Guinea Pig Hearts. Eur J Cell Biol 24: 287–297PubMedGoogle Scholar
  20. Oxhorn BC, Hirzel DJ and Buxton IL (2002): Isolation and Characterization of Large Numbers of Endothelial Cells for Studies of Cell Signalling. Microvasc Res 64: 302–315CrossRefPubMedGoogle Scholar
  21. Piper HM: Cell Culture Technique in Heart and Vessel Research. Berlin (1990): Springer-VerlagGoogle Scholar
  22. Rauen U, Hanssen M, Lauchart W, Becker HD and de Groot H (1993): Energy-Dependent Injury to Cultured Sinusoidal Endothelial Cells of the Rat Liver in UW Solution. Transplantation 55: 469–473PubMedGoogle Scholar
  23. Skalli O, Ropraz P, Trzeciak A, Benzonana G, Gillessen D and Gabbiani G (1986): A Monoclonal Antibody Against Alpha-Smooth Muscle Actin: A New Probe for Smooth Muscle Differentiation. J Cell Biol 103: 2787–2796CrossRefPubMedGoogle Scholar
  24. Slater DN and Sloan JM (1975): The Porcine Endothelial cell in Tissue Culture. Atherosclerosis 21: 259–272CrossRefPubMedGoogle Scholar
  25. Ugele B and Lange F (2001): Isolation of Endothelial Cells from Human Placental Microvessels: Effect of Different Proteolytic Enzymes on Releasing Endothelial Cells from Villous Tissue. In Vitro Cell Dev Biol Anim 37: 408–413CrossRefPubMedGoogle Scholar
  26. Watanabe H, Kuhne W, Schwartz P and Piper HM (1992): A2-Adenosine Receptor Stimulation Increases Macromolecule Permeability of Coronary Endothelial Cells. Am J Physiol 262: H1174–H1181PubMedGoogle Scholar
  27. Watanabe H, Kuhne W, Spahr R, Schwartz P and Piper HM (1991): Macromolecule Permeability of Coronary and Aortic Endothelial Monolayers Under Energy Depletion. Am J Physiol 260: H1344–H1352PubMedGoogle Scholar
  28. Watkins MT, Sharefkin JB, Zajtchuk R, Maciag TM, D’Amore PA, Ryan US, Van Wart H and Rich NM (1984): Adult Human Saphenous Vein Endothelial Cells: Assessment of Their Reproductive Capacity for use in Endothelial Seeding of Vascular Prostheses. J Surg Res 36: 588–596CrossRefPubMedGoogle Scholar

Copyright information

© Springer-Verlag Berlin Heidelberg 2005

Authors and Affiliations

  • Saskia C. Peters
    • 1
  • Anna Reis
    • 1
  • Thomas Noll
    • 1
  1. 1.Physiologisches InstitutJustus-Liebig-Universität GiessenGiessenGermany

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