Isolation and Culture of Adult Ventricular Cardiomyocytes

  • Klaus-Dieter Schlüter
  • Hans Michael Piper


Foetal Calf Serum Culture Dish Perfusion System Ventricular Cardiomyocytes Cell Culture Technique 
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  1. Pinson A, Schlüter K-D, Zhou XJ, Schwartz P, Kessler-Icekson G, Piper HM (1993) Alpha-and beta-adrenergic stimulation of protein synthesis in cultured adult ventricular cardiomyocytes. J Mol Cell Cardiol 25:477–490CrossRefPubMedGoogle Scholar
  2. Piper HM, Jacobsen SL, Schwartz P (1988) Determinants of cardiomyocyte development in long-term primary culture. J Mol Cell Cardiol 20:825–835PubMedGoogle Scholar
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  5. Piper HM, Volz A, Schwartz P (1990) Adult ventricular rat heart muscle cells. In: Piper HM (ed) Cell culture techniques in heart and vessel research. Springer Verlag, Berlin, p 36–60Google Scholar
  6. Schlüter K-D, Goldberg Y, Taimor G, Schäfer M, Piper HM (1998) Role of phosphatidyl 3-kinase activation in the hypertrophic growth of adult ventricular cardiomyocytes. Cardiovasc Res 40:174–181CrossRefPubMedGoogle Scholar
  7. Schlüter K-D, Piper HM (1992) Trophic effects of catecholamines and parathyroid hormone on adult ventricular cardiomyocytes. Am J Physiol Heart Circ Physiol 263:H1739–H1746Google Scholar
  8. Schwartz P, Piper HM, Spahr R, Hütter JF, Spiekermann PG (1985) Development of new intracellular contacts between adult cardiac myocytes in culture. Basic Res Cardiol 80[Suppl. 1]:75–78PubMedGoogle Scholar
  9. Taimor G, Lorenz H, Hofstaetter B, Schlüter K-D, Piper HM (1999) Induction of necrosis but not apoptosis after anoxia and reoxygenation in isolated adult cardiomyocytes of rat: Cardiovasc Res 41:147–156CrossRefPubMedGoogle Scholar
  10. Taimor G, Schlüter K-D, Frischkopf K, Flesch M, Rosenkranz S, Piper HM (1999) Autocrine regulation of TGFß expression in adult cardiomyocytes. J Mol Cell Cardiol 31:2127–2136CrossRefPubMedGoogle Scholar

Copyright information

© Springer-Verlag Berlin Heidelberg 2005

Authors and Affiliations

  • Klaus-Dieter Schlüter
    • 1
  • Hans Michael Piper
    • 1
  1. 1.Physiologisches InstitutJustus-Liebig-Universität GiessenGiessenGermany

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