Practical Use of Transmission Electron Microscopy for Analysis of Structures and Molecules in Cardiovascular Research

  • Wilhelm Bloch
  • Christian Hoffmann
  • Eveline Janssen
  • Yüksel Korkmaz


Gold Particle Immunoelectron Microscopy Propylene Oxide Aqueous Uranyl Acetate Maleate Buffer 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.


Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.


  1. Berryman MA, Rodewald RD (1990) An enhanced method for post-embedding immunocytochemical staining which preserves cell membranes. J Histochem Cytochem 38:159–170PubMedGoogle Scholar
  2. Bloch W, Forsberg E, Lentini S, Brakebusch C, Martin K, Krell HW, Weidle UH, Addicks K, Fässler R (1997) beta1 Integrin is essential for teratoma growth and angiogenesis. J Cell Biol. 139:265–278CrossRefPubMedGoogle Scholar
  3. Bloch W, Huggel K, Sasaki T, Grose R, Bugnon P, Addicks K, Timpl R, Werner S (2000) The angiogenesis inhibitor endostatin impairs blood vessel maturation during wound healing. FASEB J v10.1096/fj.00-0490fjeGoogle Scholar
  4. Bloch W, Mehlhorn U, Krahwinkel A, Reiner M, Dittrich M, Schmidt A, Addicks K (2001a) Ischemia increases detectable endothelial nitric oxide synthase in rat and human myocardium. Nitric Oxide 5:317–333CrossRefPubMedGoogle Scholar
  5. Bloch W, Fan Y, Han J, Xue S, Schöneberg T, Ji GJ, Zu ZJ, Walther M, Fässler R, Hescheler J, Addicks K, Fleischmann BK (2001) Disruption of cytoskeletal integrity impairs Gi-mediated signaling due to displacement of Gi-proteins. J Cell Biol 154:753–76CrossRefPubMedGoogle Scholar
  6. Brandtzaeg P (1982) Tissue preparation methods for immunohistochemistry. In: Bullock GR and Petrusz P (eds) Techniques in Immunhistochemistry, vol.1. Academic Press, New York, p 1–76Google Scholar
  7. Brorson SH (1996) Improved immunogold labeling of epoxy sections by the use of propylene oxide as additional agent in dehdyration, infiltration and embedding. Micron 27:345–353PubMedGoogle Scholar
  8. Brorson SH, Skjorten F (1996) Improved technique for immunoelectron microscopy. How to prepare epoxy resin to obtain approximately the same immunogold labeling for epoxy sections as for acrylic sections without any etching. Micron 27:211–217PubMedGoogle Scholar
  9. Brorson SH, Roos N, Skjørten F (1994) Antibody penetration into LR-White sections. Micron 25:453–460CrossRefPubMedGoogle Scholar
  10. Côté SL, Riberio-Da-Silva A, and Cuello AC (1993) Current protocols for light microscopy immunohistochemistry. In: Cuello AC (ed) Immunohistochemistry II. John Wiley & Sons, New York, p 148–167Google Scholar
  11. Costell M, Gustafsson E, Aszodi A, Mörgelin M, Bloch W, Hunziker E, Addicks K, Timpl R, Fässler R (1999) Perlecan maintains the integrity of cartilage and some basement membranes. J Cell Biol 147:1109–1122CrossRefPubMedGoogle Scholar
  12. Davies HA (1999) General preparation of material and staining of sections. Methods Mol Biol 117:1–11PubMedGoogle Scholar
  13. Hsu S-M (1993) The use of the avidin-biotin interaction in immunohistochemistry. In: Cuello AC (ed) Immunohistochemistry II. John Wiley & Sons, New York, p 169–179Google Scholar
  14. Ju H, Zou R, Venema VJ, Venema RC (1997) Direct interaction of endothelial nitric-oxide synthase and caveolin-1 inhibits synthase activity. J Biol Chem 272:18522–18525PubMedGoogle Scholar
  15. Kostka G, Giltay R, Bloch W, Addicks K, Timpl R, Fässler R, Chu ML (2001) Perinatal lethality and endothelial cell abnormalities in several vesel compartments of fibulin-1 deficient mice. Mol Cell Biol 21:7025–7034CrossRefPubMedGoogle Scholar
  16. Mehlhorn U (1997) Improved myocardial protection using continuous coronary perfusion with normothermic blood and b-blockade with esmolol. Thorac Cardiovasc Surg 45:224–231PubMedGoogle Scholar
  17. Michel JB, Feron O, Sacks D, Michel T (1997) Reciprocal regulation of endothelial nitric-oxide synthase by Ca2+-calmodulin and caveolin. J Biol Chem 272:15583–15586PubMedGoogle Scholar
  18. Oliver C, Jamur MC (1999) Fixation and embedding. Methods of Molecular Biology. 115:319–326Google Scholar
  19. Prabhakar P, Thatte HS, Goetz RM, Cho MR, Golan DE, Michel T (1998) Receptor-regulated translocation of endothelial nitric-oxide synthase. J Biol Chem 273:27383–27388CrossRefPubMedGoogle Scholar
  20. Reiner M (2002) Caveolen des kontinuierlichen Kapillarendothels. Die Anwendung eines weiterentwickelten Verfahren der hochauflösenden Immunoelektronenmikroskopie. Dissertation, Medizinische Fakultät der Universität zu KölnGoogle Scholar
  21. Reiner M, Bloch W, Addicks K (2001) Functional interaction of caveolin-1 and eNOS in myocardial capillary endothelium revealed by immunoelectron microscopy. J Histochem Cytochem 49:1605–1609PubMedGoogle Scholar
  22. Riberio-Da-Silva A, Priestley JV, and Cuello AC (1993) Pre-embedding Ultrastructural Immunocytochemistry. In: Cuello AC (ed) Immunohistochemistry II. John Wiley & Sons, New York, p 182–222Google Scholar
  23. Richardson K (1960) Studies on the structure of autonomic nerves in small intestine, correlating the silver impregnated image in light microscopy with permanganate-fixed ultrastructure in electron microscopy. J Anat 94: 457–472PubMedGoogle Scholar
  24. Roell W, Lu ZJ, Bloch W et al. (2002) Cellular cardiomyoplasty improves survival after myocardial injury. Circulation 105:2433–2439CrossRefGoogle Scholar
  25. Webster P (1999) The production of cryosections through fixed and cryoprotected biological material and their use in immunohistochemistry. Methods Mol Biol 117:49–76PubMedGoogle Scholar

Copyright information

© Springer-Verlag Berlin Heidelberg 2005

Authors and Affiliations

  • Wilhelm Bloch
    • 1
  • Christian Hoffmann
    • 2
  • Eveline Janssen
    • 2
  • Yüksel Korkmaz
    • 2
  1. 1.Institut für Kreislaufforschung und SportmedizinDeutsche Sporthochschule KölnKölnGermany
  2. 2.Zentrum Anatomie, Institut I für AnatomieUniversität zu KölnKölnGermany

Personalised recommendations