Skip to main content
SpringerLink
Log in

Immunohistochemistry for Structural and Functional Analysis in Cardiovascular Research

  • Chapter
Practical Methods in Cardiovascular Research

  • 2296 Accesses

This is a preview of subscription content, log in via an institution to check access.

Access this chapter

Log in via an institution
Chapter
USD 29.95
Price excludes VAT (USA)
  • Available as PDF
  • Read on any device
  • Instant download
  • Own it forever
eBook
USD 129.00
Price excludes VAT (USA)
  • Available as PDF
  • Read on any device
  • Instant download
  • Own it forever
Hardcover Book
USD 169.99
Price excludes VAT (USA)
  • Durable hardcover edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info

Tax calculation will be finalised at checkout

Purchases are for personal use only

Institutional subscriptions

Preview

Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.

References

  • Adams JC (1992) Biotin amplification of biotin and horseradish peroxidase signals in histochemical stains. J Histochem Cytochem 40: 1457–1463

    CAS  PubMed  Google Scholar 

  • Addicks K, Bloch W, Feelisch M (1994) Nitric oxide modulates sympathetic neurotransmission at the prejunctional level. Microscopy Research and Technique 29: 161–168

    Article  CAS  PubMed  Google Scholar 

  • Bloch W, Forsberg E, Lentini S, Brakebusch C, Martin K, Krell HW, Weidle UH, Addicks K, Fässler R (1997) Beta 1 integrin is essential for teratoma growth and angiogenesis. J Cell Biol 139: 265–278

    Article  CAS  PubMed  Google Scholar 

  • Bloch W, Mehlhorn U, Krahwinkel A, Reiner M, Dittrich M, Schmidt A, Addicks K (2001) Ischemia increases detectable endothelial nitric oxide synthase in rat and human myocardium. Nitric Oxide 5: 317–333

    Article  CAS  PubMed  Google Scholar 

  • Bloch W, Fan Y, Han J, Xue S, Schöneberg T, Ji GJ, Zu ZJ, Walther M, Fässler R, Hescheler J, Addicks K, Fleischmann BK (2001) Disruption of cytoskeletal integrity impairs Gi-mediated signaling due to displacement of Gi-proteins. J Cell Biol 154: 753–761

    Article  CAS  PubMed  Google Scholar 

  • Boenisch T (2001) Formalin-fixed and heat-retrieved tissue antigens: a comparison of their immunoreactivity in experimental antibody diluents. Appl Immunohistochem Mol Morphol 9: 176–179

    Article  CAS  PubMed  Google Scholar 

  • Böhle T, Brandt MC, Henn N, Schmidt A, Bloch W, Beuckelmann DJ (2001) Identification and in-situ registration of the cardiac ryanodine-receptor channel in membrane blebs of sarcoplasmic reticulum. FEBS Lett 505: 419–425

    Article  PubMed  Google Scholar 

  • Coons AH, Creech HJ, Jones RN (1941) Immunological properties of an antibody containing a fluorescent group. Proc Soc Exp Biol Med 47: 200–202

    CAS  Google Scholar 

  • Danscher G (1981) Localisation of gold in biological tissue: A photochemical method for light and electronmicroscopy. Histochemistry 71: 81–88

    CAS  PubMed  Google Scholar 

  • De Vente J, Steinbusch HWM (1992) On the stimulation of soluble and particulate guanylate cyclase in the rat brain and the involvement of nitric oxide as studied by cGMP immunocytochemistry. Acta Histochemistry 92: 13–38

    Google Scholar 

  • Fassler R, Pfaff M, Murphy J, Noegel AA, Johansson S, Timpl R, and Albrecht R (1995) Lack of β1 integrin gene in embryonic stem cells affects morphology, adhesion, and migration but not integration into the inner cell mass of blastocysts. J Cell Biol 128: 979–988

    Article  CAS  PubMed  Google Scholar 

  • Faulk WP, Taylor GM (1971) An immunogold method for the electron microscope. Immunohistochemistry 8: 1081–1083

    CAS  Google Scholar 

  • Fischer UW, Klass O, Stock U, Easo J, Geissler HJ, Fischer JH, Bloch W, Mehlhorn U (2003) Cardioplegic arrest induces apoptosis signal-pathway in myocardial endothelial cells and cardiac myocytes. Eur J Cardiothorac Surg 23: 984–990

    Article  PubMed  Google Scholar 

  • Fleischmann M, Bloch W, Kolossov E, Andressen C, Müller M, Brem G, Hescheler J, Addicks K, Fleischmann BK (1998) Cardiac specific expression of the green fluorescent protein during early murine embryonic development. FEBS Lett 440: 370–376

    Article  CAS  PubMed  Google Scholar 

  • Fritz P, Tuczek H, Mulhaupt H, Schwarzmann P (1995) Quantitation in immunhistochemistry. A research method or a diagnostic toll in surgical pathology. Pathologica 87: 300–309

    CAS  PubMed  Google Scholar 

  • Geoghegan WD, Scillian JJ, Ackerman GA (1978) The detection of human B-lymphocytes by both light and electron microscopy utilizing colloidal gold-labelled anti-immuno-globulin. Immunol Commun 7: 1–12

    CAS  PubMed  Google Scholar 

  • Gerdes J, Dallenbach F, Lennert K, Lemke H, Stein H (1984) Growth fraction in malignant non-Hodgkins lymphoma (NHL) as determined in situ with monoclonal antibody to Ki67. Hematol Oncol 2: 365–371

    CAS  PubMed  Google Scholar 

  • Gu J, Agrawal N (1997) Elimination of background staining in immunocytochemistry. In: Gu J (ed) Analytical morphology: theory, applications and protocols. Birkhäuser Boston, Cambridge, pp 55–68

    Google Scholar 

  • Grizzle WE, Aamodt R, Clausen K, Li Volsi V, Pretlow TG, Qualman S (1998) Providing human tissues for research: how to establish a program. Arch Pathol Lab Med 122: 1065–1076

    CAS  PubMed  Google Scholar 

  • Hacker GW, Danscher G, Hauser-Kronberger C (1997) Immunogold-silver staining — autometallography: recent developments and protocols. In: Gu J (ed) Analytical morphology: theory, applications and protocols. Birkhäuser, Boston Cambridge, pp 41–54

    Google Scholar 

  • Hainfeld JF, Furuya FR (1992) A 1.4-nm gold cluster covalently attached to antibodies improves immunolabelling. J Histochem Cytochem 40: 177–184

    CAS  PubMed  Google Scholar 

  • Hanyu Y, Ichikawa M, Matsumoto G (1992) An improved cryofixation method: cryoquenching of small tissue blocks during microwave irradiation. J Microsc 165: 255–271

    PubMed  Google Scholar 

  • Hayat MA (2002) Microscopy, immunohistochemistry, and antigen retrieval methods. Kluwer Academic/Plenium Publishers, New York

    Google Scholar 

  • Holgate CS, Jackson P, Cowen PN, Bird CC (1983) Immunogold-solver staining: new method of immunpstaining with enhanced sensitivity. J Histochem Cytochem 31: 938–944

    CAS  PubMed  Google Scholar 

  • Jackson TH, Ungan A, Critser JK, Gao D (1997) Novel microwave technology for cryopreservation of biomaterials by suppression of apparent ice formation. Cryobiology 34: 363–372

    Article  CAS  PubMed  Google Scholar 

  • Kazemi S, Wenzel D, Kolossov E, Lenka N, Raible A, Sasse P, Hescheler J, Addicks K, Fleischmann BK, Bloch W (2002) Differential role of bFGF and VEGF for vasculogenesis. Cell Physiol Biochem 12: 55–62

    Article  CAS  PubMed  Google Scholar 

  • Mehlhorn U, Bloch W, Krahwinkel A, LaRose K, Geissler HJ, Hekmat K, Addicks K, de Vivie ER (2000) Activation of myocardial constitutive nitric oxide synthase during coronary artery surgery. Eur J Cardiothorac Surg 17: 305–311

    Article  CAS  PubMed  Google Scholar 

  • Mehlhorn U, Krahwinkel A, Geissler H, LaRose K, Suedkamp M, Fischer UM, Klass O, Hekmat K, Bloch W (2003) Nitrotyrosine and 8-isoprostane formation indicate free radical-mediated injury in hearts of patients subjected to cardioplegia. J Thorac Cardiovasc Surg 125: 178–18

    Article  CAS  PubMed  Google Scholar 

  • Merz H, Malisius R, Mannweiler S, Zhou R, Hartmann W, Orscheschek K, Moubayed P, Feller AC (1995) Immunomax. A maximized immunohistochemical method for the retrieval and enhancement of hidden antigens. Lab Invest 73: 149–156

    CAS  PubMed  Google Scholar 

  • Newman SJ, Nelson P (2000) Image analysis and Statistics. In: Beesley (ed) Immunocytochemistry and in situ hybridisation in the biomedical sciences, Birkhäuser, Boston, pp 175–199

    Google Scholar 

  • Pott C, Brixius K, Bundkirchen A, Bölck B, Bloch W, Steinritz D, Mehlhorn U, Schwinger RHG (2003) The preferential β3-adrenoceptor agonist BRL 37344 increases force via β1-/β2-adrenoceptors and induces endothelial nitric oxide synthase via β3-adrenoceptors in human atrial myocardium. Br J Pharmacol 138: 521–529

    Article  CAS  PubMed  Google Scholar 

  • Ranefall P, Wester K, Andersson AC, Busch C, Bengtsson E (1998) Automatic quantification of immunohistochemically stained cell nuclei based on standard reference cells. Anal Cell Pathol 17: 111–123

    CAS  PubMed  Google Scholar 

  • Riera J, Simpson JF, Tamayo R, Battifora H (1999) Use of cultured cells as a control for quantitative immunocytochemical analysis of estrogen receptor in breast cancer. The Quicgel method. Am J Clin Pathol 111: 329–335

    CAS  PubMed  Google Scholar 

  • Roche PC, His ED (2001) Immunohistochemistry: theory and practice. In: Lloyd RV (ed) Morphology methods: cell and molecular biology techniques. Humana Press, Totowa, pp 229–237

    Google Scholar 

  • Roell W, Lu ZJ, Bloch W et al. (2002) Cellular cardiomyoplasty improves survival after myocardial injury. Circulation 105: 2433–2439

    Article  Google Scholar 

  • Roth J, Saremaslani P, Zuber C (1992) Versatility of anti-horseradish peroxidase antibody-gold coplexes for cytochemistry and in situ hybridisation: preparation and application of soluble complexes with streptavidin-peroxidase conjugates and biotinylated antibodies. Histochemistry 98: 229–236

    Article  CAS  PubMed  Google Scholar 

  • Shi SR, Key ME, Kalra KL (1991) Antigen retrieval in formalin-fixed, paraffin-embedded tissues: An enhancement method for immunohistochemical staining on microwave oven heating of tissue sections. J Histochem Cytochem 39: 741–748

    CAS  PubMed  Google Scholar 

  • Shi SR, Cote C, Kalra KL, Taylor CR, Tandon AK (1992) A technique for retrieving antigens in formalin-fixed, routinely acid-decalcified, celloidin-embedded human temporal bone sections for immunohistochemistry. J Histochem Cytochem 40: 787–792

    CAS  PubMed  Google Scholar 

  • Shi SR, Cote RJ, Yang C, Chen C, Xu HJ, Bendict WF, Taylor CR (1992) Development of an optimal for antigen retrieval: a “test battery” approach exemplified with reference to the staining of retinoblastoma protein (pRB) in formalin-fixed paraffin sections. J Pathol 179: 347–352

    Google Scholar 

  • Springall DR, Hacker GW, Grimelius, Polak JM (1984) The potential of the immunogold-silver method for paraffin sections. Histochemistry 81: 603–608

    Article  CAS  PubMed  Google Scholar 

  • Sternberger LA (1979) The unlabelled antibody peroxidase-anti-peroxidase (PAP) method. In: Sternberger LA, Immunocytochemistry, 2nd edn. John Wiley and Sons, New York, pp 104–169

    Google Scholar 

  • Taylor CR, Burns J (1974) The demonstration of plasma cells and other immunoglobulin containing cells in formalin-fixed, paraffin-embedded tissues using peroxidase labelled antibody. J Clin Pathol 27: 14–20

    CAS  PubMed  Google Scholar 

  • Taylor CR, Tandon A (1994) Theoretical and practical aspects of the different immunoperoxidase techniques. In: Taylor CR, Cote RJ (eds) Immunomicroscopy: A diagnostic tool for the surgical pathologist, WB Saunders, Philadelphia, pp 21–41

    Google Scholar 

  • Taylor CR, Shi S (2001) Antigen retrieval technique for immunohistochemistry. In: Lloyd RV (ed) Morphology methods: cell and molecular biology techniques, Humana Press, Totowa, pp 229–237

    Google Scholar 

  • Tossios P, Bloch W, Huebner A, Dodos F, Suedkamp M, Kasper S, Hellmich M, Mehlhorn U (2003) N-acetylcysteine prevents reactive oxygen species-mediated myocardial injury in cardiac surgery patients: results of a randomized double-blinded placebo-controlled clinical trial. J Thorac Cardiovasc Surg 126: 1513–1520

    Article  CAS  PubMed  Google Scholar 

  • Wobus AM, Wallukat G, Hescheler J (1991) Pluripotent mouse embryonic stem cells are able to differentiate into cardiomyocytes expressing chronotropic responses to adrenergic and cholinergic agents and Ca2+ channel blockers. Differentiation 48: 173–182

    CAS  PubMed  Google Scholar 

Download references

Author information

Authors and Affiliations

  1. Institut für Kreislaufforschung und Sportmedizin, Deutsche Sporthochschule Köln, Carl-Diem-Weg 6, 50933, Köln, Germany

    Wilhelm Bloch

  2. Zentrum Anatomie, Institut I für Anatomie, Universität zu Köln, Joseph Stelzmann Straße 9, 50931, Köln, Germany

    Yüksel Korkmaz & Dirk Steinritz

Authors
  1. Wilhelm Bloch
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Yüksel Korkmaz
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Dirk Steinritz
    View author publications

    You can also search for this author in PubMed Google Scholar

Editor information

Editors and Affiliations

  1. Klinik für Herzchirurgie, Universität Leipzig, Herzzentrum, Strümpellstraße 39, 04289, Leipzig, Germany

    Stefan Dhein  & Friedrich Wilhelm Mohr  & 

  2. Department of Pharmacology, SUNY Upstate Medical University, 766 Irving Avenue, Syracuse, NY, 13210, USA

    Mario Delmar MD PhD

Rights and permissions

Reprints and permissions

Copyright information

© 2005 Springer-Verlag Berlin Heidelberg

About this chapter

Cite this chapter

Bloch, W., Korkmaz, Y., Steinritz, D. (2005). Immunohistochemistry for Structural and Functional Analysis in Cardiovascular Research. In: Dhein, S., Mohr, F.W., Delmar, M. (eds) Practical Methods in Cardiovascular Research. Springer, Berlin, Heidelberg. https://doi.org/10.1007/3-540-26574-0_23

Download citation

  • DOI: https://doi.org/10.1007/3-540-26574-0_23

  • Publisher Name: Springer, Berlin, Heidelberg

  • Print ISBN: 978-3-540-40763-8

  • Online ISBN: 978-3-540-26574-0

  • eBook Packages: MedicineMedicine (R0)

Publish with us

Policies and ethics

Search

Navigation