Immunohistochemistry for Structural and Functional Analysis in Cardiovascular Research

  • Wilhelm Bloch
  • Yüksel Korkmaz
  • Dirk Steinritz


Antigen Retrieval Embryonic Stem Cell Line Background Staining Histological Technique Tyramide Signal Amplification 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.


Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.


  1. Adams JC (1992) Biotin amplification of biotin and horseradish peroxidase signals in histochemical stains. J Histochem Cytochem 40: 1457–1463PubMedGoogle Scholar
  2. Addicks K, Bloch W, Feelisch M (1994) Nitric oxide modulates sympathetic neurotransmission at the prejunctional level. Microscopy Research and Technique 29: 161–168CrossRefPubMedGoogle Scholar
  3. Bloch W, Forsberg E, Lentini S, Brakebusch C, Martin K, Krell HW, Weidle UH, Addicks K, Fässler R (1997) Beta 1 integrin is essential for teratoma growth and angiogenesis. J Cell Biol 139: 265–278CrossRefPubMedGoogle Scholar
  4. Bloch W, Mehlhorn U, Krahwinkel A, Reiner M, Dittrich M, Schmidt A, Addicks K (2001) Ischemia increases detectable endothelial nitric oxide synthase in rat and human myocardium. Nitric Oxide 5: 317–333CrossRefPubMedGoogle Scholar
  5. Bloch W, Fan Y, Han J, Xue S, Schöneberg T, Ji GJ, Zu ZJ, Walther M, Fässler R, Hescheler J, Addicks K, Fleischmann BK (2001) Disruption of cytoskeletal integrity impairs Gi-mediated signaling due to displacement of Gi-proteins. J Cell Biol 154: 753–761CrossRefPubMedGoogle Scholar
  6. Boenisch T (2001) Formalin-fixed and heat-retrieved tissue antigens: a comparison of their immunoreactivity in experimental antibody diluents. Appl Immunohistochem Mol Morphol 9: 176–179CrossRefPubMedGoogle Scholar
  7. Böhle T, Brandt MC, Henn N, Schmidt A, Bloch W, Beuckelmann DJ (2001) Identification and in-situ registration of the cardiac ryanodine-receptor channel in membrane blebs of sarcoplasmic reticulum. FEBS Lett 505: 419–425CrossRefPubMedGoogle Scholar
  8. Coons AH, Creech HJ, Jones RN (1941) Immunological properties of an antibody containing a fluorescent group. Proc Soc Exp Biol Med 47: 200–202Google Scholar
  9. Danscher G (1981) Localisation of gold in biological tissue: A photochemical method for light and electronmicroscopy. Histochemistry 71: 81–88PubMedGoogle Scholar
  10. De Vente J, Steinbusch HWM (1992) On the stimulation of soluble and particulate guanylate cyclase in the rat brain and the involvement of nitric oxide as studied by cGMP immunocytochemistry. Acta Histochemistry 92: 13–38Google Scholar
  11. Fassler R, Pfaff M, Murphy J, Noegel AA, Johansson S, Timpl R, and Albrecht R (1995) Lack of β1 integrin gene in embryonic stem cells affects morphology, adhesion, and migration but not integration into the inner cell mass of blastocysts. J Cell Biol 128: 979–988CrossRefPubMedGoogle Scholar
  12. Faulk WP, Taylor GM (1971) An immunogold method for the electron microscope. Immunohistochemistry 8: 1081–1083Google Scholar
  13. Fischer UW, Klass O, Stock U, Easo J, Geissler HJ, Fischer JH, Bloch W, Mehlhorn U (2003) Cardioplegic arrest induces apoptosis signal-pathway in myocardial endothelial cells and cardiac myocytes. Eur J Cardiothorac Surg 23: 984–990CrossRefPubMedGoogle Scholar
  14. Fleischmann M, Bloch W, Kolossov E, Andressen C, Müller M, Brem G, Hescheler J, Addicks K, Fleischmann BK (1998) Cardiac specific expression of the green fluorescent protein during early murine embryonic development. FEBS Lett 440: 370–376CrossRefPubMedGoogle Scholar
  15. Fritz P, Tuczek H, Mulhaupt H, Schwarzmann P (1995) Quantitation in immunhistochemistry. A research method or a diagnostic toll in surgical pathology. Pathologica 87: 300–309PubMedGoogle Scholar
  16. Geoghegan WD, Scillian JJ, Ackerman GA (1978) The detection of human B-lymphocytes by both light and electron microscopy utilizing colloidal gold-labelled anti-immuno-globulin. Immunol Commun 7: 1–12PubMedGoogle Scholar
  17. Gerdes J, Dallenbach F, Lennert K, Lemke H, Stein H (1984) Growth fraction in malignant non-Hodgkins lymphoma (NHL) as determined in situ with monoclonal antibody to Ki67. Hematol Oncol 2: 365–371PubMedGoogle Scholar
  18. Gu J, Agrawal N (1997) Elimination of background staining in immunocytochemistry. In: Gu J (ed) Analytical morphology: theory, applications and protocols. Birkhäuser Boston, Cambridge, pp 55–68Google Scholar
  19. Grizzle WE, Aamodt R, Clausen K, Li Volsi V, Pretlow TG, Qualman S (1998) Providing human tissues for research: how to establish a program. Arch Pathol Lab Med 122: 1065–1076PubMedGoogle Scholar
  20. Hacker GW, Danscher G, Hauser-Kronberger C (1997) Immunogold-silver staining — autometallography: recent developments and protocols. In: Gu J (ed) Analytical morphology: theory, applications and protocols. Birkhäuser, Boston Cambridge, pp 41–54Google Scholar
  21. Hainfeld JF, Furuya FR (1992) A 1.4-nm gold cluster covalently attached to antibodies improves immunolabelling. J Histochem Cytochem 40: 177–184PubMedGoogle Scholar
  22. Hanyu Y, Ichikawa M, Matsumoto G (1992) An improved cryofixation method: cryoquenching of small tissue blocks during microwave irradiation. J Microsc 165: 255–271PubMedGoogle Scholar
  23. Hayat MA (2002) Microscopy, immunohistochemistry, and antigen retrieval methods. Kluwer Academic/Plenium Publishers, New YorkGoogle Scholar
  24. Holgate CS, Jackson P, Cowen PN, Bird CC (1983) Immunogold-solver staining: new method of immunpstaining with enhanced sensitivity. J Histochem Cytochem 31: 938–944PubMedGoogle Scholar
  25. Jackson TH, Ungan A, Critser JK, Gao D (1997) Novel microwave technology for cryopreservation of biomaterials by suppression of apparent ice formation. Cryobiology 34: 363–372CrossRefPubMedGoogle Scholar
  26. Kazemi S, Wenzel D, Kolossov E, Lenka N, Raible A, Sasse P, Hescheler J, Addicks K, Fleischmann BK, Bloch W (2002) Differential role of bFGF and VEGF for vasculogenesis. Cell Physiol Biochem 12: 55–62CrossRefPubMedGoogle Scholar
  27. Mehlhorn U, Bloch W, Krahwinkel A, LaRose K, Geissler HJ, Hekmat K, Addicks K, de Vivie ER (2000) Activation of myocardial constitutive nitric oxide synthase during coronary artery surgery. Eur J Cardiothorac Surg 17: 305–311CrossRefPubMedGoogle Scholar
  28. Mehlhorn U, Krahwinkel A, Geissler H, LaRose K, Suedkamp M, Fischer UM, Klass O, Hekmat K, Bloch W (2003) Nitrotyrosine and 8-isoprostane formation indicate free radical-mediated injury in hearts of patients subjected to cardioplegia. J Thorac Cardiovasc Surg 125: 178–18CrossRefPubMedGoogle Scholar
  29. Merz H, Malisius R, Mannweiler S, Zhou R, Hartmann W, Orscheschek K, Moubayed P, Feller AC (1995) Immunomax. A maximized immunohistochemical method for the retrieval and enhancement of hidden antigens. Lab Invest 73: 149–156PubMedGoogle Scholar
  30. Newman SJ, Nelson P (2000) Image analysis and Statistics. In: Beesley (ed) Immunocytochemistry and in situ hybridisation in the biomedical sciences, Birkhäuser, Boston, pp 175–199Google Scholar
  31. Pott C, Brixius K, Bundkirchen A, Bölck B, Bloch W, Steinritz D, Mehlhorn U, Schwinger RHG (2003) The preferential β3-adrenoceptor agonist BRL 37344 increases force via β1-/β2-adrenoceptors and induces endothelial nitric oxide synthase via β3-adrenoceptors in human atrial myocardium. Br J Pharmacol 138: 521–529CrossRefPubMedGoogle Scholar
  32. Ranefall P, Wester K, Andersson AC, Busch C, Bengtsson E (1998) Automatic quantification of immunohistochemically stained cell nuclei based on standard reference cells. Anal Cell Pathol 17: 111–123PubMedGoogle Scholar
  33. Riera J, Simpson JF, Tamayo R, Battifora H (1999) Use of cultured cells as a control for quantitative immunocytochemical analysis of estrogen receptor in breast cancer. The Quicgel method. Am J Clin Pathol 111: 329–335PubMedGoogle Scholar
  34. Roche PC, His ED (2001) Immunohistochemistry: theory and practice. In: Lloyd RV (ed) Morphology methods: cell and molecular biology techniques. Humana Press, Totowa, pp 229–237Google Scholar
  35. Roell W, Lu ZJ, Bloch W et al. (2002) Cellular cardiomyoplasty improves survival after myocardial injury. Circulation 105: 2433–2439CrossRefGoogle Scholar
  36. Roth J, Saremaslani P, Zuber C (1992) Versatility of anti-horseradish peroxidase antibody-gold coplexes for cytochemistry and in situ hybridisation: preparation and application of soluble complexes with streptavidin-peroxidase conjugates and biotinylated antibodies. Histochemistry 98: 229–236CrossRefPubMedGoogle Scholar
  37. Shi SR, Key ME, Kalra KL (1991) Antigen retrieval in formalin-fixed, paraffin-embedded tissues: An enhancement method for immunohistochemical staining on microwave oven heating of tissue sections. J Histochem Cytochem 39: 741–748PubMedGoogle Scholar
  38. Shi SR, Cote C, Kalra KL, Taylor CR, Tandon AK (1992) A technique for retrieving antigens in formalin-fixed, routinely acid-decalcified, celloidin-embedded human temporal bone sections for immunohistochemistry. J Histochem Cytochem 40: 787–792PubMedGoogle Scholar
  39. Shi SR, Cote RJ, Yang C, Chen C, Xu HJ, Bendict WF, Taylor CR (1992) Development of an optimal for antigen retrieval: a “test battery” approach exemplified with reference to the staining of retinoblastoma protein (pRB) in formalin-fixed paraffin sections. J Pathol 179: 347–352Google Scholar
  40. Springall DR, Hacker GW, Grimelius, Polak JM (1984) The potential of the immunogold-silver method for paraffin sections. Histochemistry 81: 603–608CrossRefPubMedGoogle Scholar
  41. Sternberger LA (1979) The unlabelled antibody peroxidase-anti-peroxidase (PAP) method. In: Sternberger LA, Immunocytochemistry, 2nd edn. John Wiley and Sons, New York, pp 104–169Google Scholar
  42. Taylor CR, Burns J (1974) The demonstration of plasma cells and other immunoglobulin containing cells in formalin-fixed, paraffin-embedded tissues using peroxidase labelled antibody. J Clin Pathol 27: 14–20PubMedGoogle Scholar
  43. Taylor CR, Tandon A (1994) Theoretical and practical aspects of the different immunoperoxidase techniques. In: Taylor CR, Cote RJ (eds) Immunomicroscopy: A diagnostic tool for the surgical pathologist, WB Saunders, Philadelphia, pp 21–41Google Scholar
  44. Taylor CR, Shi S (2001) Antigen retrieval technique for immunohistochemistry. In: Lloyd RV (ed) Morphology methods: cell and molecular biology techniques, Humana Press, Totowa, pp 229–237Google Scholar
  45. Tossios P, Bloch W, Huebner A, Dodos F, Suedkamp M, Kasper S, Hellmich M, Mehlhorn U (2003) N-acetylcysteine prevents reactive oxygen species-mediated myocardial injury in cardiac surgery patients: results of a randomized double-blinded placebo-controlled clinical trial. J Thorac Cardiovasc Surg 126: 1513–1520CrossRefPubMedGoogle Scholar
  46. Wobus AM, Wallukat G, Hescheler J (1991) Pluripotent mouse embryonic stem cells are able to differentiate into cardiomyocytes expressing chronotropic responses to adrenergic and cholinergic agents and Ca2+ channel blockers. Differentiation 48: 173–182PubMedGoogle Scholar

Copyright information

© Springer-Verlag Berlin Heidelberg 2005

Authors and Affiliations

  • Wilhelm Bloch
    • 1
  • Yüksel Korkmaz
    • 2
  • Dirk Steinritz
    • 2
  1. 1.Institut für Kreislaufforschung und SportmedizinDeutsche Sporthochschule KölnKölnGermany
  2. 2.Zentrum Anatomie, Institut I für AnatomieUniversität zu KölnKölnGermany

Personalised recommendations