Recording Action Potentials Using Voltage-Sensitive Dyes

  • Vladimir G. Fast


Shot Noise Optical Mapping Dichroic Mirror Detector Noise Action Potential Amplitude 
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  1. Biermann M, Rubart M, Moreno A, Wu J, Josiah-Durant A, Zipes DP: Differential effects of cytochalasin D and 2,3 butanedione monoxime on isometric twitch force and transmembrane action potential in isolated ventricular muscle: implications for optical measurements of cardiac repolarization. J Cardiovasc Electrophysiol 9: 1348–1357, 1998PubMedGoogle Scholar
  2. Choi BR, Nho W, Liu T, Salama G: Life span of ventricular fibrillation frequencies. Circ Res 91: 339–345, 2002CrossRefPubMedGoogle Scholar
  3. Efimov IR, Cheng Y, Van Wagoner DR, Mazgalev T, Tchou PJ: Virtual electrode-induced phase singularity. A basic mechanism of defibrillation failure. Circ Res 82: 918–925, 1998PubMedGoogle Scholar
  4. Fast VG, Darrow BJ, Saffitz JE, Kleber AG: Anisotropic activation spread in heart cell monolayers assessed by highresolution optical mapping: role of tissue discontinuities. Circ Res 79: 115–127, 1996PubMedGoogle Scholar
  5. Fast VG, Ideker RE: Simultaneous optical mapping of transmembrane potential and intracellular calcium in myocyte cultures. J Cardiovasc Electrophysiol 11: 547–556, 2000Google Scholar
  6. Fast VG, Kleber AG: Microscopic conduction in cultured strands of neonatal rat heart cells measured with voltagesensitive dyes. Circ Res 73: 914–925, 1993PubMedGoogle Scholar
  7. Fast VG, Kleber AG: Anisotropic conduction in monolayers of neonatal rat heart cells cultured on collagen substrate. Circ Res 75: 591–595, 1994PubMedGoogle Scholar
  8. Fast VG, Kleber AG: Cardiac tissue geometry as a determinant of unidirectional conduction block: assessment of microscopic excitation spread by optical mapping in patterned cell cultures and in a computer model. Cardiovasc Res 29: 697–707, 1995CrossRefPubMedGoogle Scholar
  9. Fast VG, Kleber AG: Optical mapping of the effects of defibrillation shocks in cell monolayers. In: Shenasa M et al., editor. Cardiac Mapping. Futura, Armonk NY, pp 291–316, 2003Google Scholar
  10. Fast VG, Rohr S, Ideker RE: Non-linear changes of transmembrane potential caused by defibrillation shocks in strands of cultured myocytes. Am J Physiol 278: H688–H697, 2000Google Scholar
  11. Fast VG, Sharifov OF, Cheek ER, Newton J, Ideker RE: Intramural virtual electrodes during defibrillation shocks in left ventricular wall assessed by optical mapping of membrane potential. Circulation 106: 1007–1014, 2002CrossRefPubMedGoogle Scholar
  12. Frazier DW, Krassowska W, Chen P-S, Wolf PD, Daniely ND, Smith WM, Ideker RE: Transmural activations and stimulus potentials in three-dimensional anisotropic canine myocardium. Circ Res 63: 135–146, 1988PubMedGoogle Scholar
  13. Gillis AM, Fast VG, Rohr S, Kleber AG: Effects of defibrillation shocks on the spatial distribution of the transmembrane potential in strands and monolayers of cultured neonatal rat ventricular myocytes. Circ Res 79: 676–690, 1996PubMedGoogle Scholar
  14. Girouard S, Laurita K, Rosenbaum D: Unique properties of cardiac action potentials recorded with voltage-sensitive dyes. J Cardiovasc Electrophysiol 7: 1024–1038, 1996PubMedGoogle Scholar
  15. Kleber AG, Janse MJ, Fast VG: Normal and abnormal conduction in the heart. Handbook of Physiology. Section 2: The Cardiovascular System: Oxford University Press pp 455–530, 2001Google Scholar
  16. Knisley S, Baynham T: Line stimulation parallel to myofibers enhances regional uniformity of transmembrane voltage changes in rabbit hearts. Circ Res 81: 229–241, 1997PubMedGoogle Scholar
  17. Laurita K, Libbus I: Optics and detectors used in optical mapping. In: Rosenbaum D, Jalife J, editors. Optical Mapping of Cardiac Excitation and Arrhythmias. Futura, Armonk, NY, pp 61–78, 2001Google Scholar
  18. Qin H, Kay M, Chattipakorn N, Redden D, Ideker R, Rogers J: Effects of heart isolation, voltage-sensitive dye, and electromechanical uncoupling agents on ventricular fibrillation. Am J Physiol 284: H1818–H1826, 2003Google Scholar
  19. Ratzlaff EH, Grinvald A: A tandem-lens epifluorescence macroscope: hundred-fold brightness advantage for wide-field imaging. J Neurosci Methods 36: 127–137, 1991CrossRefPubMedGoogle Scholar
  20. Rosenbaum DS, Jalife J, eds. Optical Mapping of Cardiac Excitation and Arrhythmias. Futura, Armonk, NY, p 458, 2001Google Scholar
  21. Sellin LC, McArdle JJ: Multiple effects of 2,3-butanedione monoxime. Pharmacol & Toxicol 74: 305–313, 1994Google Scholar
  22. Spach MS, Miller WTI, Gezelowitz DB, Barr RC, Kootsey JM, Johnson EA: The discontinuous nature of propagation in normal canine cardiac muscle. Evidence for recurrent discontinuties of intracellular resistance that affect the membrane currents. Circ Res 48: 39–54, 1981PubMedGoogle Scholar
  23. Wharton JM, Wolf PD, Smith WM, Chen PS, Frazier DW, Yabe S, Danieley N, Ideker RE: Cardiac potential and potential gradient fields generated by single, combined, and sequential shocks during ventricular defibrillation. Circulation 85:1510–1523, 1992PubMedGoogle Scholar
  24. Wikswo JP, Lin S-F, Abbas RA: Virtual electrode effect in cardiac tissue: a common mechanism for anodal and cathodal stimulation. Biophys J 69: 2195–2210, 1995PubMedGoogle Scholar
  25. Wu J, Biermann M, Rubart M, Zipes DP: Cytochalasin D as excitation-contraction uncoupler for optically mapping action potentials in wedges of ventricular myocardium. J Cardiovasc Electrophysiol 9: 1336–1347, 1998PubMedGoogle Scholar

Copyright information

© Springer-Verlag Berlin Heidelberg 2005

Authors and Affiliations

  • Vladimir G. Fast
    • 1
  1. 1.University of Alabama at BirminghamBirminghamUSA

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