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Eine neue Methode zur quantitativen Endotoxinbestimmung mittels monoklonalem Antikörper WN1 222–5

  • Conference paper
Chirurgisches Forum 2005

Part of the book series: Deutsche Gesellschaft für Chirurgie ((FORUMBAND,volume 34))

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Abstract

The limulus amoebocyte lysate (LAL) test has been used to indicate the presence of endotoxin in plasma for many years [1]. Discordant results were explained by variations in preparation, lack of sensitivity but also specifity of the test assay [2].

In 1993 Di Padova et al. [3] were able to generate a broadly cross-reactive monoclonal antibody (Mab) Wn1-222/5 recognizing the core region of E.coli, Shigella and salmonella R-and S-form LPS. Since WN1-222/5 is capable of recognizing the immunogenic and antigenic epitope, it can be used in a therapeutical as well as in a diagnostic way.

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Literatur

  1. Levin J, Poore TE, Young NS, Margolis S, Bell WR (1972) Gram-negative sepsis: detection of endotoxemia with the LAL-test, with studies of associated changes in blood coagulation, serum lipids and complement. Ann Intern Med 76:1–7

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  2. Elin RJ, Robinson RA, Levine AS, Wolff SM (1975) Lack of clinical usefulness of the Limulus test in the diagnosis of endotoxemia. N Engl J Med 293:521–524

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  3. DiPadova FE, Brade H, Barclay GR, Poxton IR, Liehl E, Schuetze E, Kocher HP, Ramsay G, Schreier M, McClelland DBL, Rietschel ET (1993) A broadly cross-protective monoclonal antibody to Eschericia coli and Salmonella lipopoly-saccharides. Infect Immun 61:3863–3872

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Author information

Authors and Affiliations

  1. Chirurgische Klinik des Universitätsklinikums S-H Campus Lübeck, Lübeck, Deutschland

    Dr. Jan Nolde & K. Block

  2. Forschungszentrum Borstel der Universität Lübeck, Borstel, Deutschland

    H. Brade & L. Brade

  3. Novartis Pharma, Basel, Schweiz

    F. E. Di Padova

  4. Klinik für Unfallchirurgie des Universitätsklinikums S-H Campus Lübeck, Lübeck, Deutschland

    K. H. Staubach

  5. Klinik für Chirurgie, Universitätsklinikum Schleswig-Holstein, Campus Lübeck, Ratzeburger Allee 160, 23538, Lübeck

    Dr. Jan Nolde

Authors
  1. Dr. Jan Nolde
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  2. H. Brade
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  3. L. Brade
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  4. F. E. Di Padova
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  5. K. Block
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  6. K. H. Staubach
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Editor information

Editors and Affiliations

  1. Thorax- und Gefäßchirurgie, Klinikum der Philipps Universität, Baldingerstraße, 35043, Marburg

    M. Rothmund (Präsident des 122. Kongresses der Deutschen Gesellschaft für Chirurgie) (Präsident des 122. Kongresses der Deutschen Gesellschaft für Chirurgie)

  2. Klinikum Großhadern, Marchioninistraße 15, 81366, München

    K.-W. Jauch (Vorsitzender der Sektion Chirurgische Forschung) (Vorsitzender der Sektion Chirurgische Forschung)

  3. Deutsche Gesellschaft für Chirurgie Geschäftsstelle, Luisenstraße 58/59, 10117, Berlin

    H. Bauer (Generalsekretär der Deutschen Gesellschaft für Chirurgie) (Generalsekretär der Deutschen Gesellschaft für Chirurgie)

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© 2005 Springer Medizin Verlag Heidelberg

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Nolde, J., Brade, H., Brade, L., Di Padova, F.E., Block, K., Staubach, K.H. (2005). Eine neue Methode zur quantitativen Endotoxinbestimmung mittels monoklonalem Antikörper WN1 222–5. In: Rothmund, M., Jauch, KW., Bauer, H. (eds) Chirurgisches Forum 2005. Deutsche Gesellschaft für Chirurgie, vol 34. Springer, Berlin, Heidelberg. https://doi.org/10.1007/3-540-26560-0_75

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  • DOI: https://doi.org/10.1007/3-540-26560-0_75

  • Publisher Name: Springer, Berlin, Heidelberg

  • Print ISBN: 978-3-540-24888-0

  • Online ISBN: 978-3-540-26560-3

  • eBook Packages: Medicine (German Language)

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