Synchrotron radiation studies on insect flight muscle

  • Katrina J. V. Poole
  • Gert Rapp
  • Ynichiro Maeda
  • Roger S. Goody
Conference paper
Part of the Topics in Current Chemistry book series (TOPCURRCHEM, volume 147)


Insect flight muscle has proved particularly suitable for the structural investigation of the contractile proteins and their interactions. Here we review the low angle x-ray scattering experiments performed on this muscle using synchrotron radiation as a source. We briefly outline the present concensus on the cross-bridge mechanism of muscle contraction, and introduce insect muscle structure and muscle fibre diffraction. Details of x-ray beamline and camera configurations are considered with particular reference to the requirements necessary for small specimen work. A brief summary of the early experimental work on equilibrium or pseudoequilibrium states, such as that trapped by the ATP analogue AMP-PNP, is followed by a more detailed account of recent time-resolved experiments. These include measurements of the intensities of particular low-angle reflections during stretch activation and following the rapid, photolytic release of ATP from caged-ATP.


Myosin Head Layer Line Thick Filament Rigor State Virtual Source 
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Copyright information

© Springer-Verlag 1988

Authors and Affiliations

  • Katrina J. V. Poole
    • 1
  • Gert Rapp
    • 1
  • Ynichiro Maeda
    • 1
    • 2
  • Roger S. Goody
    • 1
  1. 1.Department of BiophysicsMax-Planck Institut für Medizinische ForschungHeidelbergFRG
  2. 2.EMBL OutstationHamburgFRG

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