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Regeneration of virus-free plants through in vitro culture

  • P. J. Wang
  • C. Y. Hu
Conference paper
Part of the Advances in Biochemical Engineering book series (ABE, volume 18)

Abstract

This article deals with the theories, methodology and practices of in vitro virus elimination. Viruses may be eradicated from infected stocks through meristem or callus cultures due to the low titre, or even absence, of viruses in plant meristems and what might be called “the viral elimination power of in vitro incubation”. The season and location of the buds obtained, as well as the explants' size, are important factors in virus elimination through meristem cultures. Heat treatment and high phytohormone concentrations frequently improve the results. Virus-free tissue sectors may arise from calli of infected plants. From such sectors viruse-free plants may regenerate. Nevertheless, the frequently occurring chromosome abnormalities in callus cultures may become a strong drawback for regenerating virus-free plants from callus. Rigid virus-testing procedures must be applied to reveal the virus-free status of the regenerated plants. The commonly used testing methods are briefly described. Methods employed for rapid in vitro mass-cloning of virus-free plants include: multiple shoot formation, in vitro layering, adventitious organogenesis, and in vitro storage organ induction. The resulting virus-free clones frequently exhibit an improved yield and quality. However, some of the mild virus strains display a ‘cross-protection’ effect. The removal of these viruses through tissue cultures may result in an outbreak of more severe diseases.

Keywords

Callus Culture Potato Virus Plant Tissue Culture Leaf Primordia Vitro Culture 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

Abbreviations

BA

N6-benzyladenine

CMV

cucumber mosaic virus

2,4-D

2,4-dichlorphenoxyacetic acid

GA3

gibberellic acid

IAA

3-indoleacetic acid

2iP

N6-(2-isopentenyl)-adenine

NAA

1-naphthaleneacetic acid

PVA

potato virus A

PVG

potato virus G

PVM

potato virus M

PVS

potato virus S

PVX

potato virus X

PV

potato virus Y

RNA

ribonucleic acid

TMV

tobacco mosaic virus

TYMV

turnip yellow mosaic virus

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Copyright information

© Springer Verlag 1980

Authors and Affiliations

  • P. J. Wang
    • 1
  • C. Y. Hu
    • 2
  1. 1.Institute of Botany, Academia SinicaTaipeiTaiwan (115), Republic of China
  2. 2.Biology Dept.Wm. Paterson College of N. J.WayneUSA

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