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Enzyme regulation in parsley and soybean cell cultures

  • Hahlbrock
  • J. Schroder
  • J. Vieregge
Conference paper
Part of the Advances in Biochemical Engineering book series (ABE, volume 18)

Abstract

Cell suspension cultures of parsley (Petroselinum hortense) and soybean (Glycine max) have been used for several years as model systems for enzyme regulation studies. The regulation of nitrate metabolism, of general phenylpropanoid metabolism, and of flavonoid and lignin biosynthesis have been investigated. The results obtained for these metabolic pathways in the two cultures are compared and discussed with regard to changes in various parameters related to the growth cycle of the cultures. The parameters measured include the rate of cell division, DNA, RNA, and protein concentrations, and the rates of protein synthesis and degradation.

Keywords

Cell Suspension Culture Nitrate Reductase Activity Lignin Biosynthesis Enzyme Regulation Flavonoid Biosynthesis 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

Nomenclature

ACC

Acetyl-CoA carboxylase (EC 6.4.1.2)

APT

UDP-Apiose: flavone 7-O-glucoside 2″-O-apiosyltransferase (EC 2.4.2.25)

C4H

Cinnamate 4-hydroxylase (EC 1.14.13.11)

CHI

Chalcone isomerase (EC 5.5.1.6)

CHS

Chalcone synthase (previous name: ‘flavanone synthase’)

4CL

4-Hydroxycinnamate: CoA ligase (EC 6.2.1.12)

3GT

UDP-Glucose: flavonol 3-O-glucosyltransferase (EC 2.4.1.-)

7GT

UDP-Glucose: flavonoid 7-O-glucosyltransferase (EC 2.4.1.82)

MAT

Malonyl-CoA: flavonoid glycoside malonyltransferase (EC 2.3.1.-)

3'MT

S-Adenosyl-L-methionine:flavonoid 3'-O-methyltransferase (EC 2.1.1.42)

PAL

Phenylalanine ammonialyase (EC 4.3.1.5)

RED1

Cinnamoyl-CoA: NADPH oxidoreductase (EC 1.1.1.-)

RED2

Cinnamyl alcohol: NADP oxidoreductase (EC 1.1.1.- )

UAS

UDP-Apiose synthase

kat (katal)

unit of enzyme activity: the amount of activity that converts one mole of substrate per second. According to the Recommendations of the Commission on Biochemical Nomenclature, the specific activity is expressed as kat/kg protein

mho( = S)

conductivity

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Copyright information

© Springer Verlag 1980

Authors and Affiliations

  • Hahlbrock
    • 1
  • J. Schroder
    • 1
  • J. Vieregge
    • 1
  1. 1.Biologisches Institut II der UniversitätFreiburg i.Br.Germany

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