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Mass Spectrometric Analysis of Non-canonical Cyclic Nucleotides

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Non-canonical Cyclic Nucleotides

Part of the book series: Handbook of Experimental Pharmacology ((HEP,volume 238))

Abstract

Contemporary investigations regarding the (patho)physiological roles of the non-canonical cyclic nucleoside monophosphates (cNMP) cytidine 3′,5′-cyclic monophosphate (cCMP) and uridine 3′,5′-cyclic monophosphate (cUMP) have been hampered by the lack of highly specific and sensitive analytic methods for these analytes. In addition, the existence of 2′,3′-cNMP besides 3′,5′-cNMP has been described recently. HPLC coupled with tandem mass spectrometry (HPLC-MS/MS) is the method of choice for identification and quantification of low-molecular weight endogenous metabolites. In this chapter, recommendations for an HPLC-MS/MS method for 3′,5′- and 2′,3′-cNMP are summarized.

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Author information

Authors and Affiliations

  1. Institute of Pharmacology, Hannover Medical School, Carl-Neuberg-Str. 1, 30625, Hannover, Germany

    Heike Bähre

  2. Research Core Unit Metabolomics, Hannover Medical School, Carl-Neuberg-Str. 1, 30625, Hannover, Germany

    Heike Bähre & Volkhard Kaever

Authors
  1. Heike Bähre
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  2. Volkhard Kaever
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Correspondence to Heike Bähre .

Editor information

Editors and Affiliations

  1. Medical School of Hannover Insitute of Pharmakology, Hannover, Niedersachsen, Germany

    Roland Seifert

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Bähre, H., Kaever, V. (2016). Mass Spectrometric Analysis of Non-canonical Cyclic Nucleotides. In: Seifert, R. (eds) Non-canonical Cyclic Nucleotides. Handbook of Experimental Pharmacology, vol 238. Springer, Cham. https://doi.org/10.1007/164_2016_5001

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