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Solid Phase Protein Chemical Synthesis

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Protein Ligation and Total Synthesis II

Part of the book series: Topics in Current Chemistry ((TOPCURRCHEM,volume 363))

Abstract

The chemical synthesis of peptides or small proteins is often an important step in many research projects and has stimulated the development of numerous chemical methodologies. The aim of this review is to give a substantial overview of the solid phase methods developed for the production or purification of polypeptides. The solid phase peptide synthesis (SPPS) technique has facilitated considerably the access to short peptides (<50 amino acids). However, its limitations for producing large homogeneous peptides have stimulated the development of solid phase covalent or non-covalent capture purification methods. The power of the native chemical ligation (NCL) reaction for protein synthesis in aqueous solution has also been adapted to the solid phase by the combination of novel linker technologies, cysteine protection strategies and thioester or N,S-acyl shift thioester surrogate chemistries. This review details pioneering studies and the most recent publications related to the solid phase chemical synthesis of large peptides and proteins.

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Authors and Affiliations

  1. Institut Pasteur de Lille, UMR CNRS 8161, Université de Lille, 59021, Lille, France

    Laurent Raibaut & Oleg Melnyk

  2. Université Sidi Mohamed Ben Abdellah, Fez, Morocco

    Ouafâa El Mahdi

Authors
  1. Laurent Raibaut
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  2. Ouafâa El Mahdi
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  3. Oleg Melnyk
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Corresponding author

Correspondence to Oleg Melnyk .

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Editors and Affiliations

  1. Department of Chemistry, Tsinghua University, Beijing, China

    Lei Liu

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Raibaut, L., El Mahdi, O., Melnyk, O. (2014). Solid Phase Protein Chemical Synthesis. In: Liu, L. (eds) Protein Ligation and Total Synthesis II. Topics in Current Chemistry, vol 363. Springer, Cham. https://doi.org/10.1007/128_2014_609

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