Abstract
Vibrational spectroscopy offers detailed insights, by virtue of diagnostic infrared bands, into the chemical structures and moieties which are formed during peptide fragmentation inside mass spectrometers. Over the past few years, IRMPD spectroscopy has led to a greatly improved understanding of the chemistry that takes place during collision-induced dissociation (CID) of protonated peptides. For instance, the rearrangement chemistry of b- and a-type ions, which is relevant in sequence scrambling pathways, has been directly confirmed with the technique. In this chapter, we provide a brief background on peptide fragmentation chemistry, and give an overview of areas where vibrational spectroscopy has been successfully implemented, such as CID of protonated and de-protonated peptides. We also discuss the potential of the technique for elucidating lesser-studied radical dissociation processes, such as electron capture dissociation (ECD), electron transfer dissociation (ETD), and laser photodissociation.
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References
Aebersold R, Goodlett DR (2001) Chem Rev 101:269
Steen H, Mann M (2004) Nat Rev Mol Cell Biol 5:699
Roepstorff (1984) Biomed Mass Spectrom 11:601
Yu L, Tan Y, Tsai Y, Goodlett DR, Polfer NC (2011) J Proteome Res 10:2409
Dongré AR, Jones JL, Somogyi Á, Wysocki VH (1996) J Am Chem Soc 118:8365
Tsaprailis G, Somogyi Á, Nikolaev EN, Wysocki VH (2000) Int J Mass Spectrom 195/196:467
Polfer NC, Oomens J, Suhai S, Paizs B (2007) J Am Chem Soc 129:5887
Yalcin T, Csizmadia IG, Peterson MR, Harrison AG (1996) J Am Soc Mass Spectrom 7:233
Yalcin T, Khouw C, Csizmadia IG, Peterson MR, Harrison AG (1995) J Am Soc Mass Spectrom 6:1165
Chen X, Yu L, Steill JD, Oomens J, Polfer NC (2009) J Am Chem Soc 131:18272
Yoon SH, Chamot-Rooke J, Perkins BR, Hilderbrand AE, Poutsma JC, Wysocki VH (2008) J Am Chem Soc 130:17644
Oomens J, Young S, Molesworth S, Stipdonk M (2009) J Am Soc Mass Spectrom 20:334
Gucinski AC, Chamot-Rooke J, Steinmetz V, Somogyi Á, Wysocki VH (2013) J Phys Chem A 117:1291
Zou S, Oomens J, Polfer NC (2012) Int J Mass Spectrom 316–318:12
Gucinski AC, Chamot-Rooke J, Nicol E, Somogyi Á, Wysocki VH (2012) J Phys Chem A 116:4296
Grzetic J, Oomens J (2013) J Am Soc Mass Spectrom 24:1228
Perkins BR, Chamot-Rooke J, Yoon SH, Gucinski AC, Somogyi ARD, Wysocki VH (2009) J Am Chem Soc 131:17528
Paizs B, Suhai S (2005) Mass Spectrom Rev 24:508
Rodgers MT, Ervin KM, Armentrout PB (1997) J Chem Phys 106:4499
Polfer NC, Oomens J, Suhai S, Paizs B (2005) J Am Chem Soc 127:17154
Tirado M, Polfer NC (2012) Angew Chem 124:6542
Chen X, Steill JD, Oomens J, Polfer NC (2010) J Am Soc Mass Spectrom 21:1313
Tirado M, Rutters J, Chen X, Yeung A, Maarseveen J, Eyler J, Berden G, Oomens J, Polfer N (2012) J Am Soc Mass Spectrom 23:475
Gucinski A, Somogyi Á, Chamot-Rooke J, Wysocki V (2010) J Am Soc Mass Spectrom 21:1329
Durand S, Rossa M, Hernandez O, Paizs B, Maître P (2013) J Phys Chem A 117:2508
Harrison AG, Young AB, Bleiholder C, Suhai S, Paizs B (2006) J Am Chem Soc 128:10364
Bleiholder C, Osburn S, Williams TD, Suhai S, Van Stipdonk M, Harrison AG, Paizs B (2008) J Am Chem Soc 130:17774
Goloborodko A, Gorshkov M, Good D, Zubarev R (2011) J Am Soc Mass Spectrom 22:1121
Saminathan I, Wang XS, Guo Y, Krakovska O, Voisin S, Hopkinson A, Siu KWM (2010) J Am Soc Spectrom 21:2085
Bythell BJ, Dain RP, Curtice SS, Oomens J, Steill JD, Groenewold GS, Paizs B, Van Stipdonk MJ (2010) J Phys Chem A 114:5076
Verkerk UH, Zhao J, Van Stipdonk MJ, Bythell BJ, Oomens J, Hopkinson AC, Siu KWM (2011) J Phys Chem A 115:6683
Bythell BJ, Maître P, Paizs B (2010) J Am Chem Soc 132:14766
Verkerk UH, Zhao J, Lau JK-C, Lam T-W, Hao Q, Steill JD, Siu C-K, Oomens J, Hopkinson AC, Siu KWM (2012) Int J Mass Spectrom 330/332:254
Paizs B, Bythell B, Maître P (2012) J Am Soc Mass Spectrom 23:664
Polfer NC, Bohrer BC, Plasencia MD, Paizs B, Clemmer DE (2008) J Phys Chem A 112:1286
Oomens J, Steill JD (2010) J Am Soc Mass Spectrom 21:698
Grzetic J, Oomens J (2012) Int J Mass Spectrom 316/318:216
Grzetic J, Oomens J (2012) J Am Soc Mass Spectrom 23:290
Grzetic J, Oomens J (2013) Int J Mass Spectrom 354/355:70
Bagheri-Majdi E, Ke Y, Orlova G, Chu IK, Hopkinson AC, Siu KWM (2004) J Phys Chem B 108:11170
Piatkivskyi A, Osburn S, Jaderberg K, Grzetic J, Steill J, Oomens J, Zhao J, Lau J-C, Verkerk U, Hopkinson A, Siu KWM, Ryzhov V (2013) J Am Soc Mass Spectrom 24:513
Zubarev RA, Kelleher NL, McLafferty FW (1998) J Am Chem Soc 120:3265
Syka JEP, Coon JJ, Schroeder MJ, Shabanowitz J, Hunt DF (2004) Proc Natl Acad Sci U S A 101:9528
Mirgorodskaya E, Roepstorff P, Zubarev RA (1999) Anal Chem 71:4431
Syrstad E, Turecček F (2005) J Am Soc Mass Spectrom 16:208
Anusiewicz I, Berdys-Kochanska J, Simons J (2005) J Phys Chem A 109:5801
Frison G, van der Rest G, Tureček FE, Besson T, Lemaire Jl, Maître P, Chamot-Rooke J (2008) J Am Chem Soc 130:14916
Zhang L, Reilly JP (2010) Anal Chem 82:898
Budnik BA, Haselmann KF, Zubarev RA (2001) Chem Phys Lett 342:299
Coon JJ, Shabanowitz J, Hunt DF, Syka JEP (2005) J Am Soc Mass Spectrom 16:880
Huzarska M, Ugalde I, Kaplan DA, Hartmer R, Easterling ML, Polfer NC (2010) Anal Chem 82:2873
Yoo HJ, Wang N, Zhuang S, Song H, Håkansson K (2011) J Am Chem Soc 133:16790
Rizzo TR, Stearns JA, Boyarkin OV (2009) Int Rev Phys Chem 28:481
Robertson WH, Johnson MA (2003) Annu Rev Phys Chem 54:173
Wassermann T, Boyarkin O, Paizs B, Rizzo T (2012) J Am Soc Mass Spectrom 23:1029
Acknowledgements
Some of this work was supported by the U.S. National Science Foundation under grant number CHE-0845450. Student travel support to the Netherlands for measurements with the free electron laser FELIX was provided by the NSF-PIRE (grant number OISE-0730072). This material is based upon work supported by the National Science Foundation Graduate Research Fellowship (A.P.) under Grant No. (DGE-1315138).
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Patrick, A.L., Polfer, N.C. (2014). Peptide Fragmentation Products in Mass Spectrometry Probed by Infrared Spectroscopy. In: Rijs, A., Oomens, J. (eds) Gas-Phase IR Spectroscopy and Structure of Biological Molecules. Topics in Current Chemistry, vol 364. Springer, Cham. https://doi.org/10.1007/128_2014_576
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DOI: https://doi.org/10.1007/128_2014_576
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