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Molecular Crowding and Hydration Regulating of G-Quadruplex Formation

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Book cover Quadruplex Nucleic Acids

Part of the book series: Topics in Current Chemistry ((TOPCURRCHEM,volume 330))

Abstract

Intracellular space is highly crowded with soluble and insoluble biomolecules that range from large polymers, such as proteins and nucleic acids, to small molecules, including metabolites and metal ions. It is therefore of interest to understand the effects of molecular crowding on the structure, stability, and function of biomolecules. Moreover, molecular crowding is observed not only intracellularly but also in the extracellular matrix and under the conditions used in in vitro biotechnological and nanotechnological processes. However, most biochemical studies of biomolecules are performed under dilute conditions. Recent studies have demonstrated critical effects of molecular crowding on nucleic acids. In the present study we discuss how molecular crowding affects the properties of G-quadruplexes as well as other non-canonical nucleic acid structures.

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G-quadruplexes under molecular crowding conditions

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Acknowledgments

This work was supported in part by Grants-in-Aid for Scientific Research, Scientific Research on Innovative Areas “Nanomedicine Molecular Science” (No. 2306), the “Strategic Research Foundation at Private Universities” (2009–2014), and the “Academic Frontier” project (2004–2009) from the Ministry of Education, Culture, Sports, Science and Technology, Japan, and the Hirao Taro Foundation of the Konan University Association for Academic Research. TF is a research fellow of Japan Society for the promotion of science.

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  1. FIRST (Faculty of Frontiers of Innovative Research in Science and Technology), Konan University, 7-1-20 Minatojima-Minamimachi, Chuo-ku, Kobe, 650-0047, Japan

    Daisuke Miyoshi, Takeshi Fujimoto & Naoki Sugimoto

  2. FIBER (Frontier Institute for Biomolecular Engineering Research), Konan University, 7-1-20 Minatojima-Minamimachi, Chuo-ku, Kobe, 650-0047, Japan

    Daisuke Miyoshi & Naoki Sugimoto

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  2. Takeshi Fujimoto
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  3. Naoki Sugimoto
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Correspondence to Daisuke Miyoshi or Naoki Sugimoto .

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Editors and Affiliations

  1. James Graham Brown Cancer Center, University of Louisville, Louisville, 40202, New York, USA

    Jonathan B. Chaires

  2. , Department of Chemistry, University of Alamaba at Birmingham, 3rd Ave 1530, Birmingham, 35294-1240, Alaska, USA

    David Graves

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© 2012 Springer-Verlag Berlin Heidelberg

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Miyoshi, D., Fujimoto, T., Sugimoto, N. (2012). Molecular Crowding and Hydration Regulating of G-Quadruplex Formation. In: Chaires, J., Graves, D. (eds) Quadruplex Nucleic Acids. Topics in Current Chemistry, vol 330. Springer, Berlin, Heidelberg. https://doi.org/10.1007/128_2012_335

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