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Prion Proteins pp 121-133 | Cite as

Prion Seeded Conversion and Amplification Assays

  • Christina D. Orrú
  • Byron CaugheyEmail author
Chapter
Part of the Topics in Current Chemistry book series (TOPCURRCHEM, volume 305)

Abstract

The conversion of the normal prion protein (PrPC) into its misfolded, aggregation-prone and infectious (prion) isoform is central to the progression of transmissible spongiform encephalopathies (TSEs) or prion diseases. Since the initial development of a cell free PrP conversion reaction, striking progress has been made in the development of much more continuous prion-induced conversion and amplification reactions. These studies have provided major insights into the molecular underpinnings of prion propagation and enabled the development of ultra-sensitive tests for prions and prion disease diagnosis. This chapter will provide an overview of such reactions and the practical and fundamental consequences of their development.

Keywords

Prion composition PMCA QuIC ASA Transmissible Spongiform Ecephalopathies 

Notes

Acknowledgements

We thank Dr. Jason M. Wilham for designing the figures and Anita Mora for graphics assistance.

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Copyright information

© Springer-Verlag Berlin-Heidelberg 2011

Authors and Affiliations

  1. 1.Laboratory of Persistent Viral Diseases, Rocky Mountain LaboratoriesNational Institute of Allergy and Infectious Diseases, National Institutes of HealthHamiltonUSA

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