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Basic Technologies for Droplet Microfluidics

  • Shaojiang Zeng
  • Xin Liu
  • Hua Xie
  • Bingcheng LinEmail author
Chapter
Part of the Topics in Current Chemistry book series (TOPCURRCHEM, volume 304)

Abstract

In recent years droplet microfluidics has become a quickly evolving research field. The availability of a wide range of technologies for droplet generation and manipulation has enabled the applications of droplet microfluidics in a wide variety of fields, from single cell analysis to material synthesis. In this review we summarize the main technologies for droplet microfluidics and discuss the recent advances in technologies that enable droplets as microreactors with complete functions. Applications of microdroplets in chemical reactions, particle synthesis, and single cell analysis are also briefly reviewed.

Keywords

Microdroplet Microfluidics 

Abbreviations

PDMS

Poly(dimethyl) siloxane

PCR

Polymerase chain reaction

AC

Alternating current

CE

Capillary electrophoresis

HPLC

High performance liquid chromatography

Notes

Acknowledgements

This work was supported by 973 program (No. 2007CB714505 and 2007CB714507), Knowledge Innovation Program of the Chinese Academy of Sciences (KJCX2-YW-H18), and Instrument Research and Development Program of the Chinese Academy of Sciences (YZ200908).

References

  1. 1.
    Thorsen T, Roberts RW, Arnold FH, Quake SR (2001) Phys Rev Lett 86:4163CrossRefGoogle Scholar
  2. 2.
    Anna SL, Bontoux N, Stone HA (2003) Appl Phys Lett 82:364CrossRefGoogle Scholar
  3. 3.
    Garstecki P, Fuerstman MJ, Whitesides GM (2005) Nat Phys 1:168CrossRefGoogle Scholar
  4. 4.
    Fuerstman MJ, Garstecki P, Whitesides GM (2007) Science 315:828CrossRefGoogle Scholar
  5. 5.
    Chiu DT, Lorenz RM (2009) Acc Chem Res 42:649CrossRefGoogle Scholar
  6. 6.
    Schaerli Y, Hollfelder F (2009) Mol Biosyst 5:1392CrossRefGoogle Scholar
  7. 7.
    Huebner A, Sharma S, Srisa-Art M, Hollfelder F, Edel JB, Demello AJ (2008) Lab Chip 8:1244CrossRefGoogle Scholar
  8. 8.
    Kelly BT, Baret JC, Taly V, Griffiths AD (2007) Chem Commun 1773Google Scholar
  9. 9.
    Song H, Chen DL, Ismagilov RF (2006) Angew Chem Int Ed 45:7336CrossRefGoogle Scholar
  10. 10.
    Li L, Ismagilov RF (2010) Annu Rev Biophys 39:139CrossRefGoogle Scholar
  11. 11.
    Leng J, Salmon JB (2009) Lab Chip 9:24CrossRefGoogle Scholar
  12. 12.
    Hung LH, Lee AP (2007) J Med Biol Eng 27:1Google Scholar
  13. 13.
    Park JI, Saffari A, Kumar S, Günther A, Kumacheva E (2010) Annu Rev Mater Res 40:415CrossRefGoogle Scholar
  14. 14.
    Zare RN, Kim S (2010) Annu Rev Biomed Eng 12:187CrossRefGoogle Scholar
  15. 15.
    Borland LM, Kottegoda S, Phillips KS, Allbritton NL (2008) Annu Rev Anal Chem 1:191CrossRefGoogle Scholar
  16. 16.
    Zhang Y, Ozdemir P (2009) Anal Chim Acta 638:115CrossRefGoogle Scholar
  17. 17.
    Hong J, Edel JB, deMello AJ (2009) Drug Discov Today 14:134CrossRefGoogle Scholar
  18. 18.
    Squires TM, Quake SR (2005) Rev Mod Phys 77:977CrossRefGoogle Scholar
  19. 19.
    Christopher GF, Anna SL (2007) J Phys D Appl Phys 40:R319CrossRefGoogle Scholar
  20. 20.
    Baroud CN, Gallaire F, Dangla R (2010) Lab Chip 10:2032CrossRefGoogle Scholar
  21. 21.
    Stone HA, Stroock AD, Ajdari A (2004) Annu Rev Fluid Mech 36:381CrossRefGoogle Scholar
  22. 22.
    Abdelgawad M, Wheeler AR (2009) Adv Mater 21:920CrossRefGoogle Scholar
  23. 23.
    Garstecki P, Fuerstman MJ, Stone HA, Whitesides GM (2006) Lab Chip 6:437CrossRefGoogle Scholar
  24. 24.
    Garstecki P, Stone HA, Whitesides GM (2005) Phys Rev Lett 94:164501Google Scholar
  25. 25.
    Anna SL, Mayer HC (2006) Phys Fluids 18:121512CrossRefGoogle Scholar
  26. 26.
    Dreyfus R, Tabeling P, Willaime H (2003) Phys Rev Lett 90:144505CrossRefGoogle Scholar
  27. 27.
    Shah RK, Shum HC, Rowat AC, Lee D, Agresti JJ, Utada AS, Chu LY, Kim JW, Fernandez-Nieves A, Martinez CJ, Weitz DA (2008) Mater Today 11:18CrossRefGoogle Scholar
  28. 28.
    Takeuchi S, Garstecki P, Weibel DB, Whitesides GM (2005) Adv Mater 17:1067CrossRefGoogle Scholar
  29. 29.
    Morimoto Y, Tan WH, Takeuchi S (2009) Biomed Microdevices 11:369CrossRefGoogle Scholar
  30. 30.
    Malloggi F, Vanapalli SA, Gu H, Van Den Ende D, Mugele F (2007) J Phys Condens Matter 19:462101CrossRefGoogle Scholar
  31. 31.
    Haeberle S, Zengerle R, Ducree J (2007) Microfluid Nanofluid 3:65CrossRefGoogle Scholar
  32. 32.
    Link DR, Grasland-Mongrain E, Duri A, Sarrazin F, Cheng ZD, Cristobal G, Marquez M, Weitz DA (2006) Angew Chem Int Ed 45:2556CrossRefGoogle Scholar
  33. 33.
    Baroud CN, Delville JP, Gallaire F, Wunenburger R (2007) Phys Rev E 75:046302CrossRefGoogle Scholar
  34. 34.
    Abate AR, Romanowsky MB, Agresti JJ, Weitz DA (2009) Appl Phys Lett 94:023503CrossRefGoogle Scholar
  35. 35.
    Zeng SJ, Li BW, Su XO, Qin JH, Lin BC (2009) Lab Chip 9:1340CrossRefGoogle Scholar
  36. 36.
    Brouzes E, Medkova M, Savenelli N, Marran D, Twardowski M, Hutchison JB, Rothberg JM, Link DR, Perrimon N, Samuels ML (2009) Proc Natl Acad Sci USA 106:14195CrossRefGoogle Scholar
  37. 37.
    Priest C, Herminghaus S, Seemann R (2006) Appl Phys Lett 89:3CrossRefGoogle Scholar
  38. 38.
    Mazutis L, Baret JC, Treacy P, Skhiri Y, Araghi AF, Ryckelynck M, Taly V, Griffiths AD (2009) Lab Chip 9:2902CrossRefGoogle Scholar
  39. 39.
    Mazutis L, Araghi AF, Miller OJ, Baret JC, Frenz L, Janoshazi A, Taly V, Miller BJ, Hutchison JB, Link D, Griffiths AD, Ryckelynck M (2009) Anal Chem 81:4813CrossRefGoogle Scholar
  40. 40.
    Ahn K, Agresti J, Chong H, Marquez M, Weitz DA (2006) Appl Phys Lett 88:3Google Scholar
  41. 41.
    Fidalgo LM, Abell C, Huck WTS (2007) Lab Chip 7:984CrossRefGoogle Scholar
  42. 42.
    Mazutis L, Baret JC, Griffiths AD (2009) Lab Chip 9:2665CrossRefGoogle Scholar
  43. 43.
    Niu X, Gulati S, Edel JB, deMello AJ (2008) Lab Chip 8:1837CrossRefGoogle Scholar
  44. 44.
    Tan WH, Takeuchi S (2006) Lab Chip 6:757CrossRefGoogle Scholar
  45. 45.
    Hung LH, Choi KM, Tseng WY, Tan YC, Shea KJ, Lee AP (2006) Lab Chip 6:174CrossRefGoogle Scholar
  46. 46.
    Tan YC, Fisher JS, Lee AI, Cristini V, Lee AP (2004) Lab Chip 4:292CrossRefGoogle Scholar
  47. 47.
    Song H, Tice JD, Ismagilov RF (2003) Angew Chem Int Ed 42:768CrossRefGoogle Scholar
  48. 48.
    Link DR, Anna SL, Weitz DA, Stone HA (2004) Phys Rev Lett 92:054503Google Scholar
  49. 49.
    Nie J, Kennedy RT (2010) Anal Chem 82:7852CrossRefGoogle Scholar
  50. 50.
    Yamada M, Doi S, Maenaka H, Yasuda M, Seki M (2008) J Colloid Interface Sci 321:401CrossRefGoogle Scholar
  51. 51.
    Christopher GF, Anna SL (2009) J Rheol 53:663CrossRefGoogle Scholar
  52. 52.
    Song H, Bringer MR, Tice JD, Gerdts CJ, Ismagilov RF (2003) Appl Phys Lett 83:4664CrossRefGoogle Scholar
  53. 53.
    Song H, Ismagilov RF (2003) J Am Chem Soc 125:14613CrossRefGoogle Scholar
  54. 54.
    Tice JD, Song H, Lyon AD, Ismagilov RF (2003) Langmuir 19:9127CrossRefGoogle Scholar
  55. 55.
    Bringer MR, Gerdts CJ, Song H, Tice JD, Ismagilov RF (2004) Philos Trans R Soc Lond A Math Phys Eng Sci 362:1087CrossRefGoogle Scholar
  56. 56.
    Ahn K, Kerbage C, Hunt TP, Westervelt RM, Link DR, Weitz DA (2006) Appl Phys Lett 88:024104CrossRefGoogle Scholar
  57. 57.
    Baret JC, Miller OJ, Taly V, Ryckelynck M, El-Harrak A, Frenz L, Rick C, Samuels ML, Hutchison JB, Agresti JJ, Link DR, Weitz DA, Griffiths AD (2009) Lab Chip 9:1850CrossRefGoogle Scholar
  58. 58.
    Franke T, Abate AR, Weitz DA, Wixforth A (2009) Lab Chip 9:2625CrossRefGoogle Scholar
  59. 59.
    Zhang K, Liang QL, Ma S, Mu XA, Hu P, Wang YM, Luo GA (2009) Lab Chip 9:2992CrossRefGoogle Scholar
  60. 60.
    Baroud CN, de Saint Vincent MR, Delville JP (2007) Lab Chip 7:1029CrossRefGoogle Scholar
  61. 61.
    Frenz L, Blank K, Brouzes E, Griffiths AD (2009) Lab Chip 9:1344CrossRefGoogle Scholar
  62. 62.
    Courtois F, Olguin LF, Whyte G, Bratton D, Huck WTS, Abell C, Hollfelder F (2008) Chembiochem 9:439CrossRefGoogle Scholar
  63. 63.
    Schmitz CHJ, Rowat AC, Koster S, Weitz DA (2009) Lab Chip 9:44CrossRefGoogle Scholar
  64. 64.
    Tan WH, Takeuchi S (2007) Proc Natl Acad Sci USA 104:1146CrossRefGoogle Scholar
  65. 65.
    Shi WW, Qin JH, Ye NN, Lin BC (2008) Lab Chip 8:1432CrossRefGoogle Scholar
  66. 66.
    Huebner A, Bratton D, Whyte G, Yang M, deMello AJ, Abell C, Hollfelder F (2009) Lab Chip 9:692CrossRefGoogle Scholar
  67. 67.
    Zheng B, Tice JD, Roach LS, Ismagilov RF (2004) Angew Chem Int Ed 43:2508CrossRefGoogle Scholar
  68. 68.
    Srisa-Art M, Kang DK, Hong J, Park H, Leatherbarrow RJ, Edel JB, Chang SI, de Mello AJ (2009) Chembiochem 10:1605CrossRefGoogle Scholar
  69. 69.
    Srisa-Art M, deMello AJ, Edel JB (2008) Phys Rev Lett 101:4CrossRefGoogle Scholar
  70. 70.
    Barnes SE, Cygan ZT, Yates JK, Beers KL, Amis EJ (2006) Analyst 131:1027CrossRefGoogle Scholar
  71. 71.
    Liu SJ, Gu YF, Le Roux RB, Matthews SM, Bratton D, Yunus K, Fisher AC, Huck WTS (2008) Lab Chip 8:1937CrossRefGoogle Scholar
  72. 72.
    Roman GT, Wang M, Shultz KN, Jennings C, Kennedy RT (2008) Anal Chem 80:8231CrossRefGoogle Scholar
  73. 73.
    Wang M, Roman GT, Schultz K, Jennings C, Kennedy RT (2008) Anal Chem 80:5607CrossRefGoogle Scholar
  74. 74.
    Fidalgo LM, Whyte G, Bratton D, Kaminski CF, Abell C, Huck WTS (2008) Angew Chem Int Ed 47:2042CrossRefGoogle Scholar
  75. 75.
    Fidalgo L, Whyte G, Ruotolo B, Benesch J, Stengel F, Abell C, Robinson C, Huck W (2009) Angew Chem Int Ed 48:3665CrossRefGoogle Scholar
  76. 76.
    Edgar JS, Milne G, Zhao YQ, Pabbati CP, Lim DSW, Chiu DT (2009) Angew Chem Int Ed 48:2719CrossRefGoogle Scholar
  77. 77.
    Theberge AB, Whyte G, Huck WTS (2010) Anal Chem 82:3449CrossRefGoogle Scholar
  78. 78.
    Niu XZ, Zhang B, Marszalek RT, Ces O, Edel JB, Klug DR, Demello AJ (2009) Chem Commun 6159Google Scholar
  79. 79.
    McMullen JP, Jensen KF (2009) Annu Rev Anal Chem 3:19–42Google Scholar
  80. 80.
    Tumarkin E, Kumacheva E (2009) Chem Soc Rev 38:2161CrossRefGoogle Scholar
  81. 81.
    Um E, Lee DS, Pyo HB, Park JK (2008) Microfluid Nanofluid 5:541CrossRefGoogle Scholar
  82. 82.
    Nisisako T, Torii T, Takahashi T, Takizawa Y (2006) Adv Mater 18:1152CrossRefGoogle Scholar
  83. 83.
    Li W, Greener J, Voicu D, Kumacheva E (2009) Lab Chip 9:2715CrossRefGoogle Scholar
  84. 84.
    Chiu DT (2010) Anal Bioanal Chem 397:3179–3183Google Scholar
  85. 85.
    Di Carlo D, Aghdam N, Lee LP (2006) Anal Chem 78:4925CrossRefGoogle Scholar
  86. 86.
    He MY, Edgar JS, Jeffries GDM, Lorenz RM, Shelby JP, Chiu DT (2005) Anal Chem 77:1539CrossRefGoogle Scholar
  87. 87.
    Huebner A, Olguin LF, Bratton D, Whyte G, Huck WTS, de Mello AJ, Edel JB, Abell C, Hollfelder F (2008) Anal Chem 80:3890CrossRefGoogle Scholar
  88. 88.
    Kumaresan P, Yang CJ, Cronier SA, Blazei RG, Mathies RA (2008) Anal Chem 80:3522CrossRefGoogle Scholar
  89. 89.
    Sasuga Y, Iwasawa T, Terada K, Oe Y, Sorimachi H, Ohara O, Harada Y (2008) Anal Chem 80:9141CrossRefGoogle Scholar
  90. 90.
    Yong Z, Novak R, Shuga J, Smith MT, Mathies RA (2010) Anal Chem 82:3183CrossRefGoogle Scholar
  91. 91.
    Wu HK, Wheeler A, Zare RN (2004) Proc Natl Acad Sci USA 101:12809CrossRefGoogle Scholar
  92. 92.
    Dittrich PS, Jahnz M, Schwille P (2005) Chembiochem 6:811CrossRefGoogle Scholar
  93. 93.
    Agresti JJ, Antipov E, Abate AR, Ahn K, Rowat AC, Baret JC, Marquez M, Klibanov AM, Griffiths AD, Weitz DA (2010) Proc Natl Acad Sci USA 107:4004CrossRefGoogle Scholar
  94. 94.
    Duraiswamy S, Khan SA (2009) Small 5:2828CrossRefGoogle Scholar
  95. 95.
    Churski K, Korczyk P, Garstecki P (2010) Lab Chip Miniaturisation Chem Biol 10:816CrossRefGoogle Scholar
  96. 96.
    Chen DL, Gerdts CJ, Ismagilov RF (2005) J Am Chem Soc 127:9672CrossRefGoogle Scholar
  97. 97.
    Zheng B, Gerdts CJ, Ismagilov RF (2005) Curr Opin Struct Biol 15:548CrossRefGoogle Scholar
  98. 98.
    Yadav MK, Gerdts CJ, Sanishvili R, Smith WW, Roach LS, Ismagilov RF, Kuhn P, Stevens RC (2005) J Appl Crystallogr 38:900CrossRefGoogle Scholar
  99. 99.
    Li L, Fu Q, Kors CA, Stewart L, Nollert P, Laible PD, Ismagilov RF (2010) Microfluid Nanofluid 8:789CrossRefGoogle Scholar
  100. 100.
    Kastrup CJ, Runyon MK, Lucchetta EM, Price JM, Ismagilov RF (2008) Acc Chem Res 41:549CrossRefGoogle Scholar
  101. 101.
    Meier M, Kennedy-Darling J, Choi SH, Norstrom EM, Sisodia SS, Ismagilov RF (2009) Angew Chem Int Ed 48:1487CrossRefGoogle Scholar
  102. 102.
    Shen F, Kastrup CJ, Liu Y, Ismagilov RF (2008) Arterioscler Thromb Vasc Biol 28:2035CrossRefGoogle Scholar
  103. 103.
    Kastrup CJ, Ismagilov RF (2007) J Phys Org Chem 20:711CrossRefGoogle Scholar
  104. 104.
    Gerdts CJ, Sharoyan DE, Ismagilov RF (2004) J Am Chem Soc 126:6327CrossRefGoogle Scholar
  105. 105.
    Theberge AB, Whyte G, Frenzel M, Fidalgo LM, Wootton RCR, Huck WTS (2009) Chem Commun 6225Google Scholar
  106. 106.
    deMello AJ (2006) Nature 442:394CrossRefGoogle Scholar
  107. 107.
    Marre S, Park J, Rempel J, Guan J, Bawendi MG, Jensen KF (2008) Adv Mater 20:4830CrossRefGoogle Scholar
  108. 108.
    Marre S, Jensen KF (2010) Chem Soc Rev 39:1183CrossRefGoogle Scholar
  109. 109.
    Seiffert S, Romanowsky MB, Weitz DA (2010) Langmuir 26:14842CrossRefGoogle Scholar
  110. 110.
    Nie ZH, Li W, Seo M, Xu SQ, Kumacheva E (2006) J Am Chem Soc 128:9408CrossRefGoogle Scholar
  111. 111.
    Xu SQ, Nie ZH, Seo M, Lewis P, Kumacheva E, Stone HA, Garstecki P, Weibel DB, Gitlin I, Whitesides GM (2005) Angew Chem Int Ed 44:724CrossRefGoogle Scholar
  112. 112.
    Chu LY, Utada AS, Shah RK, Kim JW, Weitz DA (2007) Angew Chem Int Ed 46:8970CrossRefGoogle Scholar
  113. 113.
    Utada AS, Lorenceau E, Link DR, Kaplan PD, Stone HA, Weitz DA (2005) Science 308:537CrossRefGoogle Scholar
  114. 114.
    Seo M, Paquet C, Nie ZH, Xu SQ, Kumacheva E (2007) Soft Matter 3:986CrossRefGoogle Scholar
  115. 115.
    Shum HC, Abate AR, Lee D, Studart AR, Wang BG, Chen CH, Thiele J, Shah RK, Krummel A, Weitz DA (2010) Macromol Rapid Commun 31:108Google Scholar
  116. 116.
    Thiele J, Abate AR, Shum HC, Bachtler S, Forster S, Weitz DA (2010) Small 6:1723CrossRefGoogle Scholar
  117. 117.
    Seiffert S, Thiele J, Abate AR, Weitz DA (2010) J Am Chem Soc 132:6606CrossRefGoogle Scholar
  118. 118.
    Schmid A, Kortmann H, Dittrich PS, Blank LM (2010) Curr Opin Biotechnol 21:12CrossRefGoogle Scholar
  119. 119.
    Edd JF, Di Carlo D, Humphry KJ, Koster S, Irimia D, Weitz DA, Toner M (2008) Lab Chip 8:1262CrossRefGoogle Scholar
  120. 120.
    Chabert M, Viovy JL (2008) Proc Natl Acad Sci USA 105:3191CrossRefGoogle Scholar
  121. 121.
    Koster S, Angile FE, Duan H, Agresti JJ, Wintner A, Schmitz C, Rowat AC, Merten CA, Pisignano D, Griffiths AD, Weitz DA (2008) Lab Chip 8:1110CrossRefGoogle Scholar
  122. 122.
    Vincent ME, Liu WS, Haney EB, Ismagilov RF (2010) Chem Soc Rev 39:974CrossRefGoogle Scholar
  123. 123.
    Boedicker JQ, Vincent ME, Ismagilov RF (2009) Angew Chem Int Ed 48:5908CrossRefGoogle Scholar
  124. 124.
    Baret JC, Beck Y, Billas-Massobrio I, Moras D, Griffiths AD (2010) Chem Biol 17:528CrossRefGoogle Scholar
  125. 125.
    Joensson HN, Samuels ML, Brouzes ER, Medkova M, Uhlen M, Link DR, Andersson-Svahn H (2009) Angew Chem Int Ed 48:2518CrossRefGoogle Scholar

Copyright information

© Springer-Verlag Berlin Heidelberg 2011

Authors and Affiliations

  • Shaojiang Zeng
    • 1
  • Xin Liu
    • 2
  • Hua Xie
    • 1
  • Bingcheng Lin
    • 1
    Email author
  1. 1.Dalian Institute of Chemical PhysicsChinese Academy of SciencesDalianChina
  2. 2.Department of ChemistryUniversity of CambridgeCambridgeUK

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