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Microfluidics pp 295-321 | Cite as

Cells in Microfluidics

  • Chi Zhang
  • Danny van NoortEmail author
Chapter
Part of the Topics in Current Chemistry book series (TOPCURRCHEM, volume 304)

Abstract

Microfluidic devices offer a realistic environment for cell cultures as it is related to scales found in biological systems. The aim is to create more in vivo like systems, in comparison to 2D plate cultures. Creating 3D cell culture constructs increase the cell’s functionality. By controlling the microenvironment (e.g., cell matrix, flow rate, temperature) cell functionality can be increased even more. As microfluidic devices allow for precise control of the microenvironment, they are a paramount tool to study stem cells and their differentiation caused by external factors. We will give an overview of the use of microfluidic devices for some biological problems, and especially as a cell culture platforms. We focus on 3D cell cultures and stem cells and their microenvironment.

Keywords

3D cell cultures Microenvironment Microfluidics Stem cells 

Abbreviations

μCCA

Microscale cell culture analogue

3D-μFCCS

Three-dimensional microfluidic cell culture system

ALP

Alkaline phosphatase

bFGF

Basic fibroblast growth factor

BMSC

Bone marrow stem cell

BSA

Bovine serum albumin

cDNA

Complementary deoxyribonucleic acid

CEPC

Circulating endothelial progenitor cell

CNS

Central nervous system

CTC

Circulating tumour cell

DC

Direct current

ECM

Extracellular matrix

EGF

Epidermal growth factor

EPC

Endothelial progenitor cell

ESC

Embryonic stem cell

FACS

Fluorescence-activated cell sorter

FBS

Fetal bovine serum

FITC

Fluorescein isothiocyanate

FSS

Flow shear stress

GFP

Green fluorescent protein

hESC

Human embryonic stem cell

hNSC

Human neural stem cell

HSC

Haematopoietic stem cell

IC50

50% maximal inhibitory concentration

iPS

Induced pluripotent stem

mRNA

Messenger ribonucleic acid

MSC

Mesenchymal stem cells

PDGF

Platelet-derived growth factor

PDMS

Polydimethylsiloxane

PEG

Polyethylene glycol

PZT

Piezoelectric transducer

SMC

Smooth muscle cell

TGF

Transforming growth factor

UHTP

Ultrahigh-throughput

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Copyright information

© Springer-Verlag Berlin Heidelberg 2011

Authors and Affiliations

  1. 1.Division of NanobiotechnologyAlbaNova University Center, Royal Institute of TechnologyStockholmSweden
  2. 2.MechanoBiology Institute, SingaporeNational University of SingaporeSingaporeSingapore

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